What is the role of ATP in kinase?
Posted May 10, 2023
Protein kinases use ATP as the source of energy to phosphorylate other proteins. The transfer of the phosphate group from ATP to its substrate protein is catalyzed by kinase. This uses the energy released by hydrolysis of ATP to fuel the reaction. The binding of ATP to the kinase induces a conformational change that activates the kinases and enables it to phosphorylate its substrate. The lack of ATP can inhibit kinase activity and slow down or stop phosphorylation of substrates. ATP is also crucial for proper folding and stability of kinases. Some kinases require ATP to keep their conformation. Other kinases utilize ATP as a cofactor for other enzymatic activities.
Catalytic Mechanisms and Regulation of Protein Kinases
Amplite® Universal Fluorimetric Kinase Assay Kit *Red Fluorescence*