AAT Bioquest

Posted on November 7, 2023
What are the most commonly used enzymes for protein detection?
Enzyme-Linked Immunosorbent Assay (ELISA)Biotin and StreptavidinHorseradish Peroxidase (HRP) and Poly-HRPWestern BlotProtein Assays & Analysis

Posted on March 4, 2022
What is affinity purification?
BioconjugationBiotin and Streptavidin

Posted on February 9, 2022
What is the difference between Immunoblot and Western Blot?
BioconjugationPhysiological ProbesBiotin and Streptavidin

Posted on December 14, 2021
How do you elute the target protein in affinity chromatography?
Physiological ProbesBioconjugationBiotin and Streptavidin

Posted on April 20, 2021
How many biotin binding-sites are there per streptavidin molecule?
BioconjugationBiotin and Streptavidin

Posted on June 1, 2020
Why streptavidin is better than avidin for binding biotin?
Antibodies and Proteomics Bioconjugation Enzyme-Linked Immunosorbent Assay (ELISA)BiotinBiotin and StreptavidinImmunohistochemistry (IHC)flow cytometryimmunofluorescence

Posted on April 17, 2020
What is the function of adding BSA as buffer in Biotin-Streptavidin system?
Antibodies and Proteomics Bioconjugation Chemical ReagentsAntibody and Protein LabelingBSABiotin and StreptavidinBuffer Preparations and Recipesbovine serum albuminbuffer