HIS Lite™ Cy5 Bis NTA Chelator
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 1203.34 |
Solvent | DMSO |
Spectral properties
Correction Factor (260 nm) | 0.02 |
Correction Factor (280 nm) | 0.03 |
Correction Factor (482 nm) | 0.009 |
Correction Factor (565 nm) | 0.09 |
Extinction coefficient (cm -1 M -1) | 2500001 |
Excitation (nm) | 651 |
Emission (nm) | 670 |
Quantum yield | 0.271, 0.42 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
Related products
Overview | SDSProtocol |
Molecular weight 1203.34 | Correction Factor (260 nm) 0.02 | Correction Factor (280 nm) 0.03 | Correction Factor (482 nm) 0.009 | Correction Factor (565 nm) 0.09 | Extinction coefficient (cm -1 M -1) 2500001 | Excitation (nm) 651 | Emission (nm) 670 | Quantum yield 0.271, 0.42 |
Cy5 Bis NTA-Ni Chelator can be applied either to an SDS-PAGE gel or Western blot membrane for fluorescence imaging or used in living cells in combination with the addition of a heavy metal ion such as Ni2+. Detection with Cy5 Bis NTA-Ni complex requires less incubation time than for protein-antibody binding. No secondary reaction is required since Ni-NTA complex is directly conjugated to the Cy5 fluorophores. Cy5 demonstrates strong fluorescent signals at ~670 nm with a common red laser excitation (e.g., He-Ne laser or 647 nm red laser). Polyhistidine is one of the most popular affinity tags incorporated into recombinant proteins. It can be inserted either at the N- or C-terminus and expressed in a variety of hosts. Due to its small size, the polyhistidine tag serves as an elegant tool for both protein purification and detection. AAT Bioquest also offers HIS Lite™ Cy5 Bis NTA-Ni Complex that can be directly used for the specific and highly sensitive detection of His-tagged fusion proteins without the addition of a heavy metal ion.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of HIS Lite™ Cy5 Bis NTA Chelator to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 83.102 µL | 415.51 µL | 831.02 µL | 4.155 mL | 8.31 mL |
5 mM | 16.62 µL | 83.102 µL | 166.204 µL | 831.02 µL | 1.662 mL |
10 mM | 8.31 µL | 41.551 µL | 83.102 µL | 415.51 µL | 831.02 µL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
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Spectrum
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Spectral properties
Correction Factor (260 nm) | 0.02 |
Correction Factor (280 nm) | 0.03 |
Correction Factor (482 nm) | 0.009 |
Correction Factor (565 nm) | 0.09 |
Extinction coefficient (cm -1 M -1) | 2500001 |
Excitation (nm) | 651 |
Emission (nm) | 670 |
Quantum yield | 0.271, 0.42 |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) | Correction Factor (482 nm) | Correction Factor (565 nm) |
HIS Lite™ Cy5 Tris NTA Chelator | 651 | 670 | 2500001 | 0.271, 0.42 | 0.02 | 0.03 | 0.009 | 0.09 |
HIS Lite™ Cy3 Bis NTA Chelator | 555 | 569 | 1500001 | 0.151 | 0.07 | 0.073 | - | - |
Images
References
View all 9 references: Citation Explorer
Expression and purification of recombinant arginine decarboxylase (speA) from Escherichia coli.
Authors: Song, Jiaping and Zhou, Chuanwen and Liu, Rui and Wu, Xudong and Wu, Di and Hu, Xiaojian and Ding, Yu
Journal: Molecular biology reports (2010): 1823-9
Authors: Song, Jiaping and Zhou, Chuanwen and Liu, Rui and Wu, Xudong and Wu, Di and Hu, Xiaojian and Ding, Yu
Journal: Molecular biology reports (2010): 1823-9
[Isolation, prokaryotic expression and activity analysis of thymidylate kinase (tmk) gene from Phytoplasma of wheat blue dwarf].
Authors: Li, Bei and Ji, Lingling and Wu, Yunfeng and Hao, Xing'an
Journal: Wei sheng wu xue bao = Acta microbiologica Sinica (2008): 739-44
Authors: Li, Bei and Ji, Lingling and Wu, Yunfeng and Hao, Xing'an
Journal: Wei sheng wu xue bao = Acta microbiologica Sinica (2008): 739-44
Bacterial elongation factors EF-Tu, their mutants, chimeric forms, and domains: isolation and purification.
Authors: Jonák, J
Journal: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences (2007): 141-53
Authors: Jonák, J
Journal: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences (2007): 141-53
[Displaying polyhistidine peptide on the cell surface of Bacillus thuringiensis by S-layer protein].
Authors: Wang, Li and Sun, Ming and Yu, Zi Niu
Journal: Shi yan sheng wu xue bao (2003): 476-81
Authors: Wang, Li and Sun, Ming and Yu, Zi Niu
Journal: Shi yan sheng wu xue bao (2003): 476-81
Lack of antitumor activity of recombinant endostatin in a human neuroblastoma xenograft model.
Authors: Jouanneau, E and Alberti, L and Nejjari, M and Treilleux, I and Vilgrain, I and Duc, A and Combaret, V and Favrot, M and Leboulch, P and Bachelot, T
Journal: Journal of neuro-oncology (2001): 11-8
Authors: Jouanneau, E and Alberti, L and Nejjari, M and Treilleux, I and Vilgrain, I and Duc, A and Combaret, V and Favrot, M and Leboulch, P and Bachelot, T
Journal: Journal of neuro-oncology (2001): 11-8
Expression and functional characterization of a pHis-tagged human bradykinin B2 receptor in COS-7 cells.
Authors: Müller, S and Adomeit, A and Kaufmann, R and Appelhans, H and Passow, H and Reissmann, S and Liebmann, C
Journal: Biological chemistry (2000): 343-7
Authors: Müller, S and Adomeit, A and Kaufmann, R and Appelhans, H and Passow, H and Reissmann, S and Liebmann, C
Journal: Biological chemistry (2000): 343-7
The cloning, expression and purification of cervine interleukin 2.
Authors: Lockhart, E and Griffin, J F and Chinn, N and Lavallie, E and Buchan, G
Journal: Cytokine (1996): 603-12
Authors: Lockhart, E and Griffin, J F and Chinn, N and Lavallie, E and Buchan, G
Journal: Cytokine (1996): 603-12
Expression of biologically active C3a as fusion proteins.
Authors: Fukuoka, Y and Tachibana, T and Yasui, A
Journal: Immunology letters (1993): 153-8
Authors: Fukuoka, Y and Tachibana, T and Yasui, A
Journal: Immunology letters (1993): 153-8
A metal-chelating piezoelectric sensor chip for direct detection and oriented immobilization of polyHis-tagged proteins.
Authors: Chen, Hsiu-Mei and Wang, Wei-Cheng and Chen, Sheng-Horng
Journal: Biotechnology progress : 1237-44
Authors: Chen, Hsiu-Mei and Wang, Wei-Cheng and Chen, Sheng-Horng
Journal: Biotechnology progress : 1237-44