HIS Lite™ iFluor™ 568 Tris NTA Chelator
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 1541.63 |
Solvent | DMSO |
Spectral properties
Correction Factor (260 nm) | 0.34 |
Correction Factor (280 nm) | 0.15 |
Extinction coefficient (cm -1 M -1) | 1000001 |
Excitation (nm) | 568 |
Emission (nm) | 587 |
Quantum yield | 0.571 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
Related products
Overview | SDSProtocol |
Molecular weight 1541.63 | Correction Factor (260 nm) 0.34 | Correction Factor (280 nm) 0.15 | Extinction coefficient (cm -1 M -1) 1000001 | Excitation (nm) 568 | Emission (nm) 587 | Quantum yield 0.571 |
iFluor® 568 Tris-NTA Chelator is used as a sensitive fluorescent probe for detecting polyhistidine-labeled proteins in combination with the addition of a heavy metal ion( such as Ni2+) in cells, solution and solid surfaces. Fluorescent tris-NTA compounds provide an efficient method for site-specific and stable noncovalent fluorescence labeling of polyhistidine-tagged proteins. In combination with OG488-tris-NTA compound iFluor® 568 Tris-NTA can be used for multicolor analysis of polyhistidine-tagged proteins. In contrast to the transient binding of conventional mono-NTA, the multivalent interaction of tris-NTA conjugated fluorophores form a much more stable complex with polyhistidine-tagged proteins. The high selectivity of tris-NTA compounds toward cumulated histidines enables the selective labeling of proteins in cell lysates and on the surface of live cells. Fluorescent tris-NTA conjugates can be applied for the analysis of a ternary protein complex in solution and on surfaces. AAT Bioquest also offers HIS Lite™ iFluor™ 568 Tris NTA-Ni Complex that can be directly used for the specific and highly sensitive detection of His-tagged fusion proteins without the addition of a heavy metal ion. The transition metal ions (e.g., Ni ion)-mediated complexation of polyhistidine-labeled proteins with fluorescent tris-NTA conjugates provides a sensitive reporter for detecting and monitoring protein-protein interactions in real time.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of HIS Lite™ iFluor™ 568 Tris NTA Chelator to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 64.866 µL | 324.332 µL | 648.664 µL | 3.243 mL | 6.487 mL |
5 mM | 12.973 µL | 64.866 µL | 129.733 µL | 648.664 µL | 1.297 mL |
10 mM | 6.487 µL | 32.433 µL | 64.866 µL | 324.332 µL | 648.664 µL |
Molarity calculator
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Spectrum
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Spectral properties
Correction Factor (260 nm) | 0.34 |
Correction Factor (280 nm) | 0.15 |
Extinction coefficient (cm -1 M -1) | 1000001 |
Excitation (nm) | 568 |
Emission (nm) | 587 |
Quantum yield | 0.571 |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) | Correction Factor (656 nm) |
HIS Lite™ iFluor™ 647 Tris NTA Chelator | 656 | 670 | 2500001 | 0.251 | 0.03 | 0.03 | 0.0793 |
Images
References
View all 33 references: Citation Explorer
Engineering Protein Nanoparticles Functionalized with an Immunodominant Coxiella burnetii Antigen to Generate a Q Fever Vaccine.
Authors: Ramirez, Aaron and Felgner, Jiin and Jain, Aarti and Jan, Sharon and Albin, Tyler J and Badten, Alexander J and Gregory, Anthony E and Nakajima, Rie and Jasinskas, Algimantas and Felgner, Philip L and Burkhardt, Amanda M and Davies, D Huw and Wang, Szu-Wen
Journal: Bioconjugate chemistry (2023): 1653-1666
Authors: Ramirez, Aaron and Felgner, Jiin and Jain, Aarti and Jan, Sharon and Albin, Tyler J and Badten, Alexander J and Gregory, Anthony E and Nakajima, Rie and Jasinskas, Algimantas and Felgner, Philip L and Burkhardt, Amanda M and Davies, D Huw and Wang, Szu-Wen
Journal: Bioconjugate chemistry (2023): 1653-1666
Protein Nanoparticle-Mediated Delivery of Recombinant Influenza Hemagglutinin Enhances Immunogenicity and Breadth of the Antibody Response.
Authors: Badten, Alexander J and Ramirez, Aaron and Hernandez-Davies, Jenny E and Albin, Tyler J and Jain, Aarti and Nakajima, Rie and Felgner, Jiin and Davies, D Huw and Wang, Szu-Wen
Journal: ACS infectious diseases (2023): 239-252
Authors: Badten, Alexander J and Ramirez, Aaron and Hernandez-Davies, Jenny E and Albin, Tyler J and Jain, Aarti and Nakajima, Rie and Felgner, Jiin and Davies, D Huw and Wang, Szu-Wen
Journal: ACS infectious diseases (2023): 239-252
A Four-Channel Surface Plasmon Resonance Sensor Functionalized Online for Simultaneous Detections of Anti-SARS-CoV-2 Antibody, Free Viral Particles, and Neutralized Viral Particles.
Authors: Dong, Tianbao and Han, Chaowei and Jiang, Meng and Zhang, Tiantian and Kang, Qing and Wang, Pengcheng and Zhou, Feimeng
Journal: ACS sensors (2022): 3560-3570
Authors: Dong, Tianbao and Han, Chaowei and Jiang, Meng and Zhang, Tiantian and Kang, Qing and Wang, Pengcheng and Zhou, Feimeng
Journal: ACS sensors (2022): 3560-3570
Regenerable and high-throughput surface plasmon resonance assay for rapid screening of anti-SARS-CoV-2 antibody in serum samples.
Authors: Jiang, Meng and Dong, Tianbao and Han, Chaowei and Liu, Luyao and Zhang, Tiantian and Kang, Qing and Wang, Pengcheng and Zhou, Feimeng
Journal: Analytica chimica acta (2022): 339830
Authors: Jiang, Meng and Dong, Tianbao and Han, Chaowei and Liu, Luyao and Zhang, Tiantian and Kang, Qing and Wang, Pengcheng and Zhou, Feimeng
Journal: Analytica chimica acta (2022): 339830
Facile Method of Tubulin Purification from Goat Brain for Reconstitution of Microtubule-Associated Intracellular Function.
Authors: Ghosh, Satyajit and Garg, Shubham and Mukherjee, Nabanita and Ghosh, Surajit
Journal: Methods in molecular biology (Clifton, N.J.) (2022): 17-45
Authors: Ghosh, Satyajit and Garg, Shubham and Mukherjee, Nabanita and Ghosh, Surajit
Journal: Methods in molecular biology (Clifton, N.J.) (2022): 17-45
Rapid and regenerable surface plasmon resonance determinations of biomarker concentration and biomolecular interaction based on tris-nitrilotriacetic acid chips.
Authors: Liu, Luyao and Han, Chaowei and Jiang, Meng and Zhang, Tiantian and Kang, Qing and Wang, Xiaoying and Wang, Pengcheng and Zhou, Feimeng
Journal: Analytica chimica acta (2021): 338625
Authors: Liu, Luyao and Han, Chaowei and Jiang, Meng and Zhang, Tiantian and Kang, Qing and Wang, Xiaoying and Wang, Pengcheng and Zhou, Feimeng
Journal: Analytica chimica acta (2021): 338625
Quantifying the Interaction of Phosphite with ABC Transporters: MicroScale Thermophoresis and a Novel His-Tag Labeling Approach.
Authors: Bartoschik, Tanja and Gupta, Amit and Kern, Beate and Hitchcock, Andrew and Adams, Nathan B P and Tschammer, Nuska
Journal: Methods in molecular biology (Clifton, N.J.) (2020): 51-62
Authors: Bartoschik, Tanja and Gupta, Amit and Kern, Beate and Hitchcock, Andrew and Adams, Nathan B P and Tschammer, Nuska
Journal: Methods in molecular biology (Clifton, N.J.) (2020): 51-62
Near-native, site-specific and purification-free protein labeling for quantitative protein interaction analysis by MicroScale Thermophoresis.
Authors: Bartoschik, Tanja and Galinec, Stefanie and Kleusch, Christian and Walkiewicz, Katarzyna and Breitsprecher, Dennis and Weigert, Sebastian and Muller, Yves A and You, Changjiang and Piehler, Jacob and Vercruysse, Thomas and Daelemans, Dirk and Tschammer, Nuska
Journal: Scientific reports (2018): 4977
Authors: Bartoschik, Tanja and Galinec, Stefanie and Kleusch, Christian and Walkiewicz, Katarzyna and Breitsprecher, Dennis and Weigert, Sebastian and Muller, Yves A and You, Changjiang and Piehler, Jacob and Vercruysse, Thomas and Daelemans, Dirk and Tschammer, Nuska
Journal: Scientific reports (2018): 4977
Docking of Antibodies into the Cavities of DNA Origami Structures.
Authors: Ouyang, Xiangyuan and De Stefano, Mattia and Krissanaprasit, Abhichart and Bank Kodal, Anne Louise and Bech Rosen, Christian and Liu, Tianqiang and Helmig, Sarah and Fan, Chunhai and Gothelf, Kurt V
Journal: Angewandte Chemie (International ed. in English) (2017): 14423-14427
Authors: Ouyang, Xiangyuan and De Stefano, Mattia and Krissanaprasit, Abhichart and Bank Kodal, Anne Louise and Bech Rosen, Christian and Liu, Tianqiang and Helmig, Sarah and Fan, Chunhai and Gothelf, Kurt V
Journal: Angewandte Chemie (International ed. in English) (2017): 14423-14427
Affinity Capturing and Surface Enrichment of a Membrane Protein Embedded in a Continuous Supported Lipid Bilayer.
Authors: Gunnarsson, Anders and Simonsson Nyström, Lisa and Burazerovic, Sabina and Gunnarsson, Jenny and Snijder, Arjan and Geschwindner, Stefan and Höök, Fredrik
Journal: ChemistryOpen (2016): 445-449
Authors: Gunnarsson, Anders and Simonsson Nyström, Lisa and Burazerovic, Sabina and Gunnarsson, Jenny and Snijder, Arjan and Geschwindner, Stefan and Höök, Fredrik
Journal: ChemistryOpen (2016): 445-449