ReadiCleave™ Cy5-SSL-NHS ester

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Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Shipping	Standard overnight for United States, inquire for international

Physical properties

Molecular weight	1018.33
Solvent	DMSO

Spectral properties

Correction Factor (260 nm)	0.02
Correction Factor (280 nm)	0.03
Correction Factor (482 nm)	0.009
Correction Factor (565 nm)	0.09
Extinction coefficient (cm ^-1 M ^-1)	250000¹
Excitation (nm)	651
Emission (nm)	670
Quantum yield	0.27¹, 0.4²

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure

Overview SDS Protocol

Molecular weight

1018.33

Correction Factor (260 nm)

0.02

Correction Factor (280 nm)

0.03

Correction Factor (482 nm)

0.009

Correction Factor (565 nm)

0.09

Extinction coefficient (cm ^-1 M ^-1)

250000¹

Excitation (nm)

651

Emission (nm)

670

Quantum yield

0.27¹, 0.4²

Fluorescence-based methods have many advantages for biological detections in terms of sensitivity and convenience. Many biological molecules can be readily labeled with a fluorescent tag for fluorescence imaging and flow cytometry analysis. However, most of the existing fluorescent tags are used to permanently labeling biological targets from which the added fluorescent tags cannot be cleaved for further downstream analysis, such as mass spectral analysis or another detection mode. AAT Bioquest’s ReadiCleave™ linkers enable fluorescent tags conjugated to a biological target from which the added fluorescent tag can be removed when needed. ReadiCleave™ Cy5-SSL-NHS ester contains a S-S linker that can be cleaved with TCEP or DTT to remove the Cy5 fluorophore from the target molecule. The cleavage can be carried out by adding DTT or TCEP solution and incubating from room temperature to 65 °C for 1-10 min.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of ReadiCleave™ Cy5-SSL-NHS ester to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	98.2 µL	491 µL	982 µL	4.91 mL	9.82 mL
5 mM	19.64 µL	98.2 µL	196.4 µL	982 µL	1.964 mL
10 mM	9.82 µL	49.1 µL	98.2 µL	491 µL	982 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Spectrum

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Spectral properties

Correction Factor (260 nm)	0.02
Correction Factor (280 nm)	0.03
Correction Factor (482 nm)	0.009
Correction Factor (565 nm)	0.09
Extinction coefficient (cm ^-1 M ^-1)	250000¹
Excitation (nm)	651
Emission (nm)	670
Quantum yield	0.27¹, 0.4²

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Quantum yield	Correction Factor (280 nm)
ReadiCleave™ AML Cy5 NHS ester	651	670	250000¹	0.27¹, 0.4²	0.03
ReadiCleave™ XFD532 SSL-NHS ester	534	553	81000	0.61¹	0.09
ReadiCleave™ FAM SSL-NHS ester	491	516	73000	0.92	0.35

Images

References

View all 50 references: Citation Explorer

Repurposing an atherosclerosis targeting peptide for tumor imaging.
Authors: Kovacs, Luciana and Davis, Ryan A and Ganguly, Tanushree and Chammas, Roger and Sutcliffe, Julie L
Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie (2022): 112469

Small molecule based EGFR targeting of biodegradable nanoparticles containing temozolomide and Cy5 dye for greatly enhanced image-guided glioblastoma therapy.
Authors: Schmitt, Rebecca R and Mahajan, Supriya D and Pliss, Artem and Prasad, Paras N
Journal: Nanomedicine : nanotechnology, biology, and medicine (2022): 102513

Nucleic Acid-Gated Covalent Organic Frameworks for Cancer-Specific Imaging and Drug Release.
Authors: Gao, Peng and Shen, Xiaoying and Liu, Xiaohan and Chen, Yuanyuan and Pan, Wei and Li, Na and Tang, Bo
Journal: Analytical chemistry (2021): 11751-11757

Near-Infrared Bioluminescence Imaging of Animal Cells with Through-Bond Energy Transfer Cassette.
Authors: Abe, Masahiro and Nishihara, Ryo and Kim, Sung-Bae and Suzuki, Koji
Journal: Methods in molecular biology (Clifton, N.J.) (2021): 103-110

On-resin strategy to label α-conotoxins: Cy5-RgIA, a potent α9α10 nicotinic acetylcholine receptor imaging probe.
Authors: Muttenthaler, Markus and Nevin, Simon T and Inserra, Marco and Lewis, Richard J and Adams, David J and Alewood, Paul
Journal: Australian journal of chemistry (2020): 327-333

Ultrafast Excitation Transfer in Cy5 DNA Photonic Wires Displays Dye Conjugation and Excitation Energy Dependency.
Authors: Mazuski, Richard J and Díaz, Sebastián A and Wood, Ryan E and Lloyd, Lawson T and Klein, William P and Mathur, Divita and Melinger, Joseph S and Engel, Gregory S and Medintz, Igor L
Journal: The journal of physical chemistry letters (2020): 4163-4172

Synthesis and Evaluation of New Trivalent Ligands for Hepatocyte Targeting via the Asialoglycoprotein Receptor.
Authors: Reshitko, Galina S and Yamansarov, Emil Yu and Evteev, Sergei A and Lopatukhina, Elena V and Shkil', Dmitry O and Saltykova, Irina V and Lopukhov, Anton V and Kovalev, Sergey V and Lobov, Alexander N and Kislyakov, Ivan V and Burenina, Olga Yu and Klyachko, Natalia L and Garanina, Anastasiia S and Dontsova, Olga A and Ivanenkov, Yan A and Erofeev, Alexander S and Gorelkin, Peter V and Beloglazkina, Elena K and Majouga, Alexander G
Journal: Bioconjugate chemistry (2020): 1313-1319

Fluorescence background quenching as a means to increase Signal to Background ratio - a proof of concept during Nerve Imaging.
Authors: Buckle, Tessa and van der Wal, Steffen and van Willigen, Danny M and Aalderink, Germaine and KleinJan, Gijs H and van Leeuwen, Fijs W B
Journal: Theranostics (2020): 9890-9898

Mapping Fluorescence Enhancement of Plasmonic Nanorod Coupled Dye Molecules.
Authors: Tóth, Emese and Ungor, Ditta and Novák, Tibor and Ferenc, Györgyi and Bánhelyi, Balázs and Csapó, Edit and Erdélyi, Miklós and Csete, Mária
Journal: Nanomaterials (Basel, Switzerland) (2020)

A split aptamer sensing platform for highly sensitive detection of theophylline based on dual-color fluorescence colocalization and single molecule photobleaching.
Authors: Liu, Shi Gang and Zhang, Dong and He, Yu and Gao, Wenli and Shi, Xingbo
Journal: Biosensors & bioelectronics (2020): 112461