ReadiCleave™ iFluor 488 AML-NHS ester
Ordering information
Price | |
Catalog Number | |
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 1340.49 |
Solvent | DMSO |
Spectral properties
Correction Factor (260 nm) | 0.21 |
Correction Factor (280 nm) | 0.11 |
Extinction coefficient (cm -1 M -1) | 750001 |
Excitation (nm) | 491 |
Emission (nm) | 516 |
Quantum yield | 0.91 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Related products
Overview | SDSProtocol |
Molecular weight 1340.49 | Correction Factor (260 nm) 0.21 | Correction Factor (280 nm) 0.11 | Extinction coefficient (cm -1 M -1) 750001 | Excitation (nm) 491 | Emission (nm) 516 | Quantum yield 0.91 |
Fluorescence-based methods have many advantages for biological detections in terms of sensitivity and convenience. Many biological molecules can be readily labeled with a fluorescent tag for fluorescence imaging and flow cytometry analysis. However, most of the existing fluorescent tags are used to permanently labeling biological targets from which the added fluorescent tags cannot be cleaved for further downstream analysis, such as mass spectral analysis. AAT Bioquest’s ReadiCleave™ linkers enable fluorescent tags conjugated to a biological target from which the added fluorescent tag can be removed when needed. This ReadiCleave™ AML iFluor® 488 contains an azidomethyl linker that can be cleaved with TCEP to remove the iFluor® 488 fluorophore from the target molecule. The cleavage can be carried out by adding 10 mM TCEP solution (pH 7.5), and incubating at 65 °C for 1-5 min. iFluor® 488 is a superior replacement to Alexa Fluor® 488. iFluor® 488 and Alexa Fluor® 488 have very similar spectral properties.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of ReadiCleave™ iFluor 488 AML-NHS ester to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 74.6 µL | 372.998 µL | 745.996 µL | 3.73 mL | 7.46 mL |
5 mM | 14.92 µL | 74.6 µL | 149.199 µL | 745.996 µL | 1.492 mL |
10 mM | 7.46 µL | 37.3 µL | 74.6 µL | 372.998 µL | 745.996 µL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
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Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
Correction Factor (260 nm) | 0.21 |
Correction Factor (280 nm) | 0.11 |
Extinction coefficient (cm -1 M -1) | 750001 |
Excitation (nm) | 491 |
Emission (nm) | 516 |
Quantum yield | 0.91 |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
ReadiCleave™ iFluor 700 AML-NHS ester | 690 | 713 | 2200001 | 0.231 | 0.09 | 0.04 |
ReadiCleave™ iFluor 546 AML-NHS ester | 541 | 557 | 1000001 | 0.671 | 0.25 | 0.15 |
ReadiCleave™ iFluor 594 AML-NHS ester | 587 | 603 | 2000001 | 0.531 | 0.05 | 0.04 |
ReadiCleave™ iFluor 488 SSL-NHS ester | 491 | 516 | 750001 | 0.91 | 0.21 | 0.11 |
References
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Gold nanoparticles enhance fluorescence signals by flow cytometry at low antibody concentrations.
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Journal: Journal of materials chemistry. B (2021): 1414-1423
Authors: Reis, Daniela S and de Oliveira, Vivian L and Silva, Misael L and Paniago, Roberto M and Ladeira, Luiz O and Andrade, Lidia M
Journal: Journal of materials chemistry. B (2021): 1414-1423
Highly sensitive detection of DNA methyltransferase activity and its inhibitor screening by coupling fluorescence correlation spectroscopy with polystyrene polymer dots.
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Journal: The Analyst (2021)
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Authors: Lu, Ju and Zong, Shenfei and Wang, Zhuyuan and Chen, Chen and Zhang, Yizhi and Wang, Hong and Cui, Yiping
Journal: ACS omega (2021): 8808-8815
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Journal: ACS omega (2021): 8808-8815
Towards analyzing the potential of exosomes to deliver microRNA therapeutics.
Authors: Giassafaki, Lefki-Pavlina N and Siqueira, Scheyla and Panteris, Emmanuel and Psatha, Konstantina and Chatzopoulou, Fani and Aivaliotis, Michalis and Tzimagiorgis, Georgios and Müllertz, Anette and Fatouros, Dimitrios G and Vizirianakis, Ioannis S
Journal: Journal of cellular physiology (2021): 1529-1544
Authors: Giassafaki, Lefki-Pavlina N and Siqueira, Scheyla and Panteris, Emmanuel and Psatha, Konstantina and Chatzopoulou, Fani and Aivaliotis, Michalis and Tzimagiorgis, Georgios and Müllertz, Anette and Fatouros, Dimitrios G and Vizirianakis, Ioannis S
Journal: Journal of cellular physiology (2021): 1529-1544
Co-adsorption of synthetic Mincle agonists and antigen to silica nanoparticles for enhanced vaccine activity: A formulation approach to co-delivery.
Authors: Abdelwahab, Walid M and Riffey, Alexander and Buhl, Cassie and Johnson, Craig and Ryter, Kendal and Evans, Jay T and Burkhart, David J
Journal: International journal of pharmaceutics (2021): 120119
Authors: Abdelwahab, Walid M and Riffey, Alexander and Buhl, Cassie and Johnson, Craig and Ryter, Kendal and Evans, Jay T and Burkhart, David J
Journal: International journal of pharmaceutics (2021): 120119
Exchange of Proteins in Liposomes through Streptolysin O Pores.
Authors: Tsuji, Gakushi and Sunami, Takeshi and Oki, Masaya and Ichihashi, Norikazu
Journal: Chembiochem : a European journal of chemical biology (2021)
Authors: Tsuji, Gakushi and Sunami, Takeshi and Oki, Masaya and Ichihashi, Norikazu
Journal: Chembiochem : a European journal of chemical biology (2021)
Fluorescence anisotropy study of radiation-induced DNA damage clustering based on FRET.
Authors: Akamatsu, Ken and Shikazono, Naoya and Saito, Takeshi
Journal: Analytical and bioanalytical chemistry (2021): 1185-1192
Authors: Akamatsu, Ken and Shikazono, Naoya and Saito, Takeshi
Journal: Analytical and bioanalytical chemistry (2021): 1185-1192
Metal-enhanced sensing platform for the highly sensitive detection of C-reactive protein antibody and rhodamine B with applications in cardiovascular diseases and food safety.
Authors: Thao, Nguyen Thanh and Hoang, Thi Xoan and Phan, Thang Bach and Kim, Jae Young and Ta, Hanh Kieu Thi and Trinh, Kieu The Loan and Tran, Nhu Hoa Thi
Journal: Dalton transactions (Cambridge, England : 2003) (2021)
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Journal: Dalton transactions (Cambridge, England : 2003) (2021)
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Authors: Kousik, Shravan R and Sipp, Diane and Abitaev, Karina and Li, Yawen and Sottmann, Thomas and Koynov, Kaloian and Atanasova, Petia
Journal: Nanomaterials (Basel, Switzerland) (2021)
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Journal: Nanomaterials (Basel, Switzerland) (2021)
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Authors: Hugelier, S and Van den Eynde, R and Vandenberg, W and Dedecker, P
Journal: Talanta (2021): 122117
Application notes
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
Abbreviation of Common Chemical Compounds Related to Peptides
Bright Tide Fluor™-Based Fluorescent Peptides and Their Applications In Drug Discovery and Disease Diagnosis
FITC (Fluorescein isothiocyanate)
Fluorescein isothiocyanate (FITC)
Abbreviation of Common Chemical Compounds Related to Peptides
Bright Tide Fluor™-Based Fluorescent Peptides and Their Applications In Drug Discovery and Disease Diagnosis
FITC (Fluorescein isothiocyanate)
Fluorescein isothiocyanate (FITC)