ReadiCleave™ ROXtra AML-NHS ester
Ordering information
Price | |
Catalog Number | |
Unit Size | |
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 1379.58 |
Solvent | DMSO |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Related products
Overview | SDSProtocol |
Molecular weight 1379.58 |
Fluorescence-based methods have many advantages for biological detections in terms of sensitivity and convenience. Many biological molecules can be readily labeled with a fluorescent tag for fluorescence imaging and flow cytometry analysis. However, most of the existing fluorescent tags are used to permanently labeling biological targets from which the added fluorescent tags cannot be cleaved for further downstream analysis, such as mass spectral analysis. AAT Bioquest’s ReadiCleave™ linkers enable fluorescent tags conjugated to a biological target from which the added fluorescent tag can be removed when needed. This ReadiCleave™ AML ROXtra contains an azidomethyl linker that can be cleaved with TCEP to remove the ROXtra fluorophore from the target molecule. The cleavage can be carried out by adding 10 mM TCEP solution (pH 7.5), and incubating at 65 °C for 1-5 min. ROXtra is the newly developed fluorophore that has the same spectra to the standard ROX dye with significantly improve stability and water solubility.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of ReadiCleave™ ROXtra AML-NHS ester to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 72.486 µL | 362.429 µL | 724.858 µL | 3.624 mL | 7.249 mL |
5 mM | 14.497 µL | 72.486 µL | 144.972 µL | 724.858 µL | 1.45 mL |
10 mM | 7.249 µL | 36.243 µL | 72.486 µL | 362.429 µL | 724.858 µL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
ReadiCleave™ AML Cy5 NHS ester | 651 | 670 | 2500001 | 0.271, 0.42 | 0.02 | 0.03 |
ReadiCleave™ XFD532 AML-NHS ester | 534 | 553 | 81000 | 0.611 | 0.24 | 0.09 |
ReadiCleave™ FITC AML-NHS ester | 498 | 517 | 800001 | 0.79001, 0.952 | 0.32 | 0.35 |
ReadiCleave™ XFD488 AML-NHS ester | 499 | 520 | 73000 | 0.921 | 0.3 | 0.11 |
ReadiCleave™ XFD546 AML-NHS ester | 561 | 572 | 112000 | 0.791 | 0.21 | 0.12 |
ReadiCleave™ XFD647 AML-NHS ester | 650 | 671 | 239000 | 0.331 | 0.00 | 0.03 |
References
View all 50 references: Citation Explorer
Development of a PCR-lateral flow assay for rapid detection of Yersinia pestis, the causative agent of plague: PCR-LF assay for Yersinia pestis.
Authors: Singh, Rita and Pal, Vijai and Kumar, Manoj and Tripathi, N K and Goel, A K
Journal: Acta tropica (2021): 105958
Authors: Singh, Rita and Pal, Vijai and Kumar, Manoj and Tripathi, N K and Goel, A K
Journal: Acta tropica (2021): 105958
Testing of four-sample pools offers resource optimization without compromising diagnostic performance of real time reverse transcriptase-PCR assay for COVID-19.
Authors: Singh, Anirudh K and Nema, Ram Kumar and Joshi, Ankur and Shankar, Prem and Gupta, Sudheer and Yadav, Ashvini Kumar and Nema, Shashwati and Mathew, Bijina J and Shrivas, Arti and Patankar, Chitra and Raghuwanshi, Arun and Pandey, Ritu and Tripathi, Ranu and Ansari, Kudsia and Singh, Kuldeep and Yadav, Jogender and Biswas, Debasis and Singh, Sarman
Journal: PloS one (2021): e0251891
Authors: Singh, Anirudh K and Nema, Ram Kumar and Joshi, Ankur and Shankar, Prem and Gupta, Sudheer and Yadav, Ashvini Kumar and Nema, Shashwati and Mathew, Bijina J and Shrivas, Arti and Patankar, Chitra and Raghuwanshi, Arun and Pandey, Ritu and Tripathi, Ranu and Ansari, Kudsia and Singh, Kuldeep and Yadav, Jogender and Biswas, Debasis and Singh, Sarman
Journal: PloS one (2021): e0251891
Disruptors, a new class of oligonucleotide reagents, significantly improved PCR performance on templates containing stable intramolecular secondary structures.
Authors: Liu, Zhaocheng and Zhao, Chengxue and Zhao, Guodong and Xiong, Shangmin and Ma, Yong and Zheng, Minxue
Journal: Analytical biochemistry (2021): 114169
Authors: Liu, Zhaocheng and Zhao, Chengxue and Zhao, Guodong and Xiong, Shangmin and Ma, Yong and Zheng, Minxue
Journal: Analytical biochemistry (2021): 114169
Laboratory Diagnosis of 37 Cases of Bartonella Endocarditis Based on Enzyme Immunoassay and Real-Time PCR.
Authors: Shapira, Lev and Rasis, Michal and Binsky Ehrenreich, Inbal and Maor, Yasmin and Katchman, Eugene A and Treves, Adi and Velan, Ariel and Halutz, Ora and Graidy-Varon, Merav and Leibovitch, Cecilia and Maisler, Noam and Ephros, Moshe and Giladi, Michael
Journal: Journal of clinical microbiology (2021)
Authors: Shapira, Lev and Rasis, Michal and Binsky Ehrenreich, Inbal and Maor, Yasmin and Katchman, Eugene A and Treves, Adi and Velan, Ariel and Halutz, Ora and Graidy-Varon, Merav and Leibovitch, Cecilia and Maisler, Noam and Ephros, Moshe and Giladi, Michael
Journal: Journal of clinical microbiology (2021)
Real-time PCR-based Y-specific sperm quantification assay in Queensland fruit fly: Insights to patterns of sperm storage.
Authors: Shadmany, J and Lee, S F and Taylor, P W
Journal: Insect molecular biology (2021): 315-324
Authors: Shadmany, J and Lee, S F and Taylor, P W
Journal: Insect molecular biology (2021): 315-324
SERS-based viral load quantification of hepatitis B virus from PCR products.
Authors: Batool, Fatima and Nawaz, Haq and Majeed, Muhammad Irfan and Rashid, Nosheen and Bashir, Saba and Akbar, Saba and Abubakar, Muhammad and Ahmad, Shamsheer and Ashraf, Muhammad Naeem and Ali, Saqib and Kashif, Muhammad and Amin, Imran
Journal: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy (2021): 119722
Authors: Batool, Fatima and Nawaz, Haq and Majeed, Muhammad Irfan and Rashid, Nosheen and Bashir, Saba and Akbar, Saba and Abubakar, Muhammad and Ahmad, Shamsheer and Ashraf, Muhammad Naeem and Ali, Saqib and Kashif, Muhammad and Amin, Imran
Journal: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy (2021): 119722
Genetic identification of medicinally used Salacia species by nrDNA ITS sequences and a PCR-RFLP assay for authentication of Salacia-related health foods.
Authors: Zhu, Shu and Liu, Qundong and He, Jingyu and Nakajima, Naomi and Samarakoon, S P and Swe, Swe and Zaw, Khin and Komatsu, Katsuko
Journal: Journal of ethnopharmacology (2021): 113909
Authors: Zhu, Shu and Liu, Qundong and He, Jingyu and Nakajima, Naomi and Samarakoon, S P and Swe, Swe and Zaw, Khin and Komatsu, Katsuko
Journal: Journal of ethnopharmacology (2021): 113909
High-resolution melting PCR assay as a powerful tool for the epidemiological surveillance of tularemia in Western Europe.
Authors: Kevin, Maëllys and Girault, Guillaume and Caspar, Yvan and Cherfa, Moulay Ali and Mendy, Christiane and Maurin, Max and Ponsart, Claire and Madani, Nora
Journal: Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases (2021): 104741
Authors: Kevin, Maëllys and Girault, Guillaume and Caspar, Yvan and Cherfa, Moulay Ali and Mendy, Christiane and Maurin, Max and Ponsart, Claire and Madani, Nora
Journal: Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases (2021): 104741
SARS-CoV-2 viral load dynamics and real-time RT-PCR cycle threshold interpretation in symptomatic non-hospitalised individuals in New Zealand: a multicentre cross sectional observational study.
Authors: Fox-Lewis, Andrew and Fox-Lewis, Shivani and Beaumont, Jenna and Drinković, Dragana and Harrower, Jay and Howe, Kevin and Jackson, Catherine and Rahnama, Fahimeh and Shilton, Blair and Qiao, Helen and Smith, Kevin K and Morpeth, Susan C and Taylor, Susan and Blakiston, Matthew and Roberts, Sally and McAuliffe, Gary
Journal: Pathology (2021): 530-535
Authors: Fox-Lewis, Andrew and Fox-Lewis, Shivani and Beaumont, Jenna and Drinković, Dragana and Harrower, Jay and Howe, Kevin and Jackson, Catherine and Rahnama, Fahimeh and Shilton, Blair and Qiao, Helen and Smith, Kevin K and Morpeth, Susan C and Taylor, Susan and Blakiston, Matthew and Roberts, Sally and McAuliffe, Gary
Journal: Pathology (2021): 530-535
A public website for the automated assessment and validation of SARS-CoV-2 diagnostic PCR assays.
Authors: Li, Po-E and Myers Y Gutiérrez, Adán and Davenport, Karen and Flynn, Mark and Hu, Bin and Lo, Chien-Chi and Player Jackson, Elais and Shakya, Migun and Xu, Yan and Gans, Jason D and Chain, Patrick S G
Journal: Bioinformatics (Oxford, England) (2021): 1024-1025
Authors: Li, Po-E and Myers Y Gutiérrez, Adán and Davenport, Karen and Flynn, Mark and Hu, Bin and Lo, Chien-Chi and Player Jackson, Elais and Shakya, Migun and Xu, Yan and Gans, Jason D and Chain, Patrick S G
Journal: Bioinformatics (Oxford, England) (2021): 1024-1025
Application notes
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
Abbreviation of Common Chemical Compounds Related to Peptides
Bright Tide Fluor™-Based Fluorescent Peptides and Their Applications In Drug Discovery and Disease Diagnosis
FITC (Fluorescein isothiocyanate)
Fluorescein isothiocyanate (FITC)
Abbreviation of Common Chemical Compounds Related to Peptides
Bright Tide Fluor™-Based Fluorescent Peptides and Their Applications In Drug Discovery and Disease Diagnosis
FITC (Fluorescein isothiocyanate)
Fluorescein isothiocyanate (FITC)