Rhod-4™ alkyne

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Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Shipping	Standard overnight for United States, inquire for international

Physical properties

Molecular weight	852.85
Solvent	DMSO

Spectral properties

Excitation (nm)	523
Emission (nm)	551
Quantum yield	0.1¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

Alternative formats

Overview SDS Protocol

Molecular weight

852.85

Excitation (nm)

523

Emission (nm)

551

Quantum yield

0.1¹

Rhod-4™ alkyne is an excellent building block that can be readily used to prepare a calcium-sensitive bioconjugate for monitoring calcium change spatially for a specific target. It can be conjugated to azido-containing biomolecules via the well-known click chemistry. Rhod-4™ is a fluorescent calcium indicator dye commonly used in cell biology and neuroscience research. It belongs to the family of rhodol-based calcium. Rhod-4™ is a high-affinity calcium indicator dye that exhibits changes in fluorescence intensity upon binding to calcium ions. When Rhod-4™ binds to calcium, its fluorescence intensity increases dramatically, allowing researchers to measure and monitor changes in calcium levels. Compared to the traditional Rhod-2, Rhod-4™ is a superior replacement. It is a valuable tool in calcium imaging research, enabling the detection and quantification of intracellular calcium dynamics. It's fluorescence properties and calcium binding specificity make it suitable for studying calcium signaling in various cell types and biological systems.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Rhod-4™ alkyne to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	117.254 µL	586.27 µL	1.173 mL	5.863 mL	11.725 mL
5 mM	23.451 µL	117.254 µL	234.508 µL	1.173 mL	2.345 mL
10 mM	11.725 µL	58.627 µL	117.254 µL	586.27 µL	1.173 mL

Molarity calculator

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Spectrum

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Spectral properties

Excitation (nm)	523
Emission (nm)	551
Quantum yield	0.1¹

Product Family

Name	Excitation (nm)	Emission (nm)	Quantum yield
Rhod-4™, AM	523	551	0.1¹

Images

Figure 1. Rhod-4™ alkyne is an excellent building block that can be readily used to prepare a calcium-sensitive bioconjugate for monitoring calcium change spatially for a specific target. It can be conjugated to azido-containing biomolecule via the well-known click chemistry.

References

View all 50 references: Citation Explorer

Mitochondria-Endoplasmic Reticulum Contact Sites Dynamics and Calcium Homeostasis Are Differentially Disrupted in PINK1-PD or PRKN-PD Neurons.
Authors: Grossmann, Dajana and Malburg, Nina and Glaß, Hannes and Weeren, Veronika and Sondermann, Verena and Pfeiffer, Julia F and Petters, Janine and Lukas, Jan and Seibler, Philip and Klein, Christine and Grünewald, Anne and Hermann, Andreas
Journal: Movement disorders : official journal of the Movement Disorder Society (2023)

Palmitic acid methyl ester induces cardiac hypertrophy through activating the GPR receptor-mediated changes of intracellular calcium concentrations and mitochondrial functions.
Authors: Lien, Chih-Feng and Chiu, Hung-Wen and Lee, Wen-Sen and Lin, Jian-Hong and Wang, Yi-Shun and Ting, Pei-Ching and Luo, Yu-Po and Chang, Jui-Chih and Yang, Kun-Ta
Journal: Journal of cellular physiology (2023): 242-256

Cisplatin induces damage of auditory cells: Possible relation with dynamic variation in calcium homeostasis and responding channels.
Authors: Zhao, Hao and Xu, Yue and Song, Xinlei and Zhang, Qingchen and Wang, Yajie and Yin, Haiyan and Bai, Xiaohui and Li, Jianfeng
Journal: European journal of pharmacology (2022): 174662

Inhibitory Effects of 6,8-Diprenylorobol on Endometriosis Progression in Humans by Disrupting Calcium Homeostasis and Mitochondrial Function.
Authors: Song, Jisoo and Song, Gwonhwa and Park, Sunwoo and Lim, Whasun
Journal: Antioxidants (Basel, Switzerland) (2022)

NiONPs-induced alteration in calcium signaling and mitochondrial function in pulmonary artery endothelial cells involves oxidative stress and TRPV4 channels disruption.
Authors: Germande, Ophélie and Baudrimont, Magalie and Beaufils, Fabien and Freund-Michel, Véronique and Ducret, Thomas and Quignard, Jean-François and Errera, Marie-Hélène and Lacomme, Sabrina and Gontier, Etienne and Mornet, Stéphane and Bejko, Megi and Muller, Bernard and Marthan, Roger and Guibert, Christelle and Deweirdt, Juliette and Baudrimont, Isabelle
Journal: Nanotoxicology (2022): 29-51

The Effect of Primary Root Canal Treatment on Dentinal Tubule Penetration of Calcium Silicate-based Sealers during Endodontic Retreatment.
Authors: Eğemen, Ayşenur and Belli, Sema
Journal: Journal of endodontics (2022): 1169-1177

Decabromodiphenyl ether initiates mitochondria-dependent apoptosis by disrupting calcium homeostasis in mice livers.
Authors: Che, Siyan and Chen, Sunni and Li, Shiqi and Ruan, Zheng
Journal: Chemosphere (2022): 132767

Whole brain irradiation in mice causes long-term impairment in astrocytic calcium signaling but preserves astrocyte-astrocyte coupling.
Authors: Institoris, Adam and Murphy-Royal, Ciaran and Tarantini, Stefano and Yabluchanskiy, Andriy and Haidey, Jordan N and Csiszar, Anna and Ungvari, Zoltan and Gordon, Grant R
Journal: GeroScience (2021): 197-212

Visualization of Dynamic Mitochondrial Calcium Fluxes in Isolated Cardiomyocytes.
Authors: Krstic, Anna Maria and Power, Amelia Sally and Ward, Marie-Louise
Journal: Frontiers in physiology (2021): 808798

Local recovery of cardiac calcium-induced calcium release interrogated by ultra-effective, two-photon uncaging of calcium.
Authors: Janicek, Radoslav and Agarwal, Hitesh and Gómez, Ana M and Egger, Marcel and Ellis-Davies, Graham C R and Niggli, Ernst
Journal: The Journal of physiology (2021): 3841-3852