Rhod-4™ amine

Ordering information

Price
Catalog Number
Unit Size
Quantity

Add to cart

Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Shipping	Standard overnight for United States, inquire for international

Physical properties

Molecular weight	856.76
Solvent	DMSO

Spectral properties

Excitation (nm)	523
Emission (nm)	551
Quantum yield	0.1¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

Alternative formats

Overview SDS Protocol

Molecular weight

856.76

Excitation (nm)

523

Emission (nm)

551

Quantum yield

0.1¹

Rhod-4™ amine is an excellent building block that can be readily used to prepare a calcium-sensitive bioconjugate for monitoring calcium change spatially for a specific target. It can be conjugated to carboxy-containing biomolecules via activated esters or EDC chemistry. Rhod-4™ is a fluorescent calcium indicator dye commonly used in cell biology and neuroscience research. It belongs to the family of rhodol-based calcium. Rhod-4™ is a high-affinity calcium indicator dye that exhibits changes in fluorescence intensity upon binding to calcium ions. When Rhod-4™ binds to calcium, its fluorescence intensity increases dramatically, allowing researchers to measure and monitor changes in calcium levels. Compared to the traditional Rhod-2, Rhod-4™ is a superior replacement. It is a valuable tool in calcium imaging research, enabling the detection and quantification of intracellular calcium dynamics. It's fluorescence properties and calcium binding specificity make it suitable for studying calcium signaling in various cell types and biological systems.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Rhod-4™ amine to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	116.719 µL	583.594 µL	1.167 mL	5.836 mL	11.672 mL
5 mM	23.344 µL	116.719 µL	233.438 µL	1.167 mL	2.334 mL
10 mM	11.672 µL	58.359 µL	116.719 µL	583.594 µL	1.167 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
	/		=		x		=

Spectrum

Open in Advanced Spectrum Viewer

Spectral properties

Excitation (nm)	523
Emission (nm)	551
Quantum yield	0.1¹

Product Family

Name	Excitation (nm)	Emission (nm)	Quantum yield
Rhod-4™, AM	523	551	0.1¹

Images

Figure 1. Rhod-4™ amine is an excellent building block that can be readily used to prepare a calcium-sensitive bioconjugate for monitoring calcium change spatially for a specific target. It can be conjugated to caboxy-containing biomolecule via activated esters or EDC chemistry.

References

View all 6 references: Citation Explorer

Cigarette smoke extraxt influences intracellular calcium concentration in A549 cells.
Authors: Yoo, Y-M and Jung, E-M and Jeon, B-H and Tran, D N and Jeung, E-B
Journal: Journal of physiology and pharmacology : an official journal of the Polish Physiological Society (2020)

Dexamethasone Treatment Increases the Intracellular Calcium Level Through TRPV6 in A549 Cells.
Authors: Jeon, Bo-Hui and Yoo, Yeong-Min and Jung, Eui-Man and Jeung, Eui-Bae
Journal: International journal of molecular sciences (2020)

A comparison of fluorescent Ca²⁺ indicators for imaging local Ca²⁺ signals in cultured cells.
Authors: Lock, Jeffrey T and Parker, Ian and Smith, Ian F
Journal: Cell calcium (2015): 638-48

Analysis of normal and rcdl Irish setter retinal proteins.
Authors: Cunnick, J and Takemoto, D
Journal: Progress in clinical and biological research (1989): 441-54

Rod/cone dysplasia in Irish setters. Presence of an altered rhodopsin.
Authors: Cunnick, J and Rider, M and Takemoto, L J and Takemoto, D J
Journal: The Biochemical journal (1988): 335-41

Antisera to synthetic peptides of bovine rhodopsin: use as site-specific probes of disc membrane changes in retinal dystrophic dogs.
Authors: Takemoto, D J and Spooner, B and Takemoto, L J
Journal: Biochemical and biophysical research communications (1985): 438-44