Enzyme DetectionNucleic Acid DetectionProtein BiochemistrySmall Molecule Detection
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Biochemical Assays

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Related: ELISA, in vitro, horseradish peroxidase, microplate

Biochemical assays are analytical tools created for in vitro applications. They can be used to characterize, track and quantify a variety of biomolecules. These include small molecules, such as GSH and NAD, as well as macromolecules like proteins.

Biochemical assays typically contain two parts. The first is a substrate-specific probe which identifies the target of interest. This probe is usually an antibody or small molecule chelator. The second part is the detection system, which turns the probe into a quantifiable signal. One popular detection system is horseradish peroxidase (HRP) used in tandem with an oxidizing agent such as ADHP.


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Biochemical detection systems broadly fall into three categories. There are colorimetric or chromogenic assays, which undergo a visible color change. There are fluorometric or fluorogenic assays, which emit signals only upon excitation by a light source. And there are luminescent assays, which emit light by way of a chemical reaction.

Biochemical assays have a wide range of applications. They are used for ELISA, Western blotting, and other immunohistochemistry assays. Signals generated from these applications can be detected and measured using a colorimeter, fluorimeter or fluorescence microscope.

At AAT Bioquest, we provide a comprehensive variety of biochemical assays for investigating enzymes, nucleic acids and other analytes. Our catalog features reagents and kits that are optimized for seamless experimental application and that help end-users obtain results to further understanding of cellular and biomolecular functions.


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