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Biochemical Assays
AAT Bioquest provides a comprehensive portfolio of ready-to-use biochemical assays and immunoassay reagents for quantitative detection across multiple instrument platforms. Our Amplite® and Portelite™ assay kits deliver exceptional sensitivity for measuring metabolites, enzyme activities, and cell signaling molecules, while our drug conjugate portfolio supports development of competitive ELISAs for food safety, forensic toxicology, and pharmaceutical testing.
AAT Bioquest Assay Technologies
Amplite® — High-throughput fluorimetric and colorimetric kits for microplate readers
Portelite™ — Qubit™ and CytoCite™ compatible kits for fast, single-tube biochemical readouts
Portelite™ — Qubit™ and CytoCite™ compatible kits for fast, single-tube biochemical readouts
Browse Biochemical Assays
Fluorimetric Assays (Microplate Reader)
Fluorimetric microplate assays offer the highest sensitivity for detecting low-abundance metabolites, enzyme activities, and cellular functions in 96-well and 384-well formats. Amplite® fluorogenic substrates generate bright, stable signals across multiple wavelength options (blue, green, red, NIR) to minimize sample interference.
- Enzyme activity assays for peroxidases, oxidases, phosphatases, proteases, and dehydrogenases
- Metabolite quantitation including glucose, lactate, NAD/NADH, cholesterol, and triglycerides
- ROS and oxidative stress detection with H₂O₂, superoxide, and nitric oxide probes
- Cell viability, apoptosis, and calcium flux assays for HTS applications
- Nucleic acid quantitation with Helixyte™ and StrandBrite™ DNA/RNA dyes
Fluorimetric Assays (Qubit™ and Cytocite™)
Portelite™ fluorometer assays provide rapid, single-tube quantitation of nucleic acids, proteins, ions, and specialty analytes using minimal sample volumes. Portelite™ kits are specifically optimized for CytoCite™ and Qubit™ fluorometers and typically come provided with assay standards for calibration.
- High-sensitivity and broad-range DNA quantitation for NGS library preparation
- RNA and total nucleic acid quantitation for sequencing workflows
- Protein quantitation with broad compatibility across antibody types
- Lithium and sodium ion quantification for therapeutic drug monitoring
- Biotin and endotoxin detection for conjugate QC and contamination screening
Colorimetric Assays (Microplate Reader)
Colorimetric microplate assays deliver reliable absorbance-based detection compatible with standard spectrophotometers and high-throughput workflows. These assays are ideal for laboratories with absorbance-only plate readers or applications where sample color may interfere with fluorescence.
- Cell viability assays including CCK-8, MTT, MTS, and XTT for cytotoxicity screening
- Energy metabolism quantitation for glucose, lactate, pyruvate, and ATP
- Redox status assessment with NAD/NADH and NADP/NADPH ratio kits
- Enzyme activity assays for ALT, AST, LDH, AChE, and lipase
- Protein quantitation using BCA, Bradford, and Lowry methods
Colorimetric Assays (NanoDrop)
NanoDrop-optimized colorimetric assays enable rapid micro-volume measurements using only 1-2 µL of sample. These specialized assays are formulated for accurate readings on NanoDrop and other micro-volume spectrophotometers.
- Biotin quantitation for determining biotin:protein ratios on conjugates
- Protein aldehyde content measurement for site-selective modification analysis
- Micro-volume pH measurement using Spexyte™ probes (pH 4.5-8.5)
- No plate reader required—results in seconds with minimal sample consumption
ELISA Assays (Microplate Reader)
ELISA detection reagents include complete assay kits, drug-protein conjugates and ready-to-use substrate solutions for HRP-based immunoassays.
- Complete ELISA kits for development of assays using mouse and rabbit primary antibodies
- ReadiUse™ TMB and ABTS substrate solutions pre-optimized for HRP conjugates
- Drug-BSA, drug-KLH and drug-HRP conjugates for ELISA assay development
- Compatible with standard ELISA workflows
This document (01.0247.251203r1) was last updated on Sat Feb 28 2026. All trademarks and registered trademarks mentioned herein are the property of their respective owners.