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Biochemical assays are analytical tools created for in vitro applications. They can be used to characterize, track and quantify a variety of biomolecules. These include small molecules, such as GSH and NAD, as well as macromolecules like proteins.
Biochemical assays typically contain two parts. The first is a substrate-specific probe which identifies the target of interest. This probe is usually an antibody or small molecule chelator. The second part is the detection system, which turns the probe into a quantifiable signal. One popular detection system is horseradish peroxidase (HRP) used in tandem with an oxidizing agent such as ADHP.
Biochemical detection systems broadly fall into three categories. There are colorimetric or chromogenic assays, which undergo a visible color change. There are fluorometric or fluorogenic assays, which emit signals only upon excitation by a light source. And there are luminescent assays, which emit light by way of a chemical reaction.
Biochemical assays have a wide range of applications. They are used for ELISA, Western blotting, and other immunohistochemistry assays. Signals generated from these applications can be detected and measured using a colorimeter, fluorimeter or fluorescence microscope.
At AAT Bioquest, we provide a comprehensive variety of biochemical assays for investigating enzymes, nucleic acids and other analytes. Our catalog features reagents and kits that are optimized for seamless experimental application and that help end-users obtain results to further understanding of cellular and biomolecular functions.