AAT Bioquest offers a comprehensive portfolio of fluorimetric assay kits optimized for microplate reader detection, enabling researchers to quantify metabolites, enzyme activities, and signaling-related metabolites and cofactors with high sensitivity and throughput. The Amplite® platform forms the foundation of this collection, featuring proprietary fluorogenic substrates that deliver exceptional signal-to-noise ratios across multiple wavelength options. These ready-to-use kits are designed for 96-well and 384-well formats, supporting applications from basic research to high-throughput drug screening.

Amplite® enzyme activity assay kits utilize highly sensitive fluorogenic substrates for accurate measurement of enzyme kinetics and inhibitor potency. One mechanism for achieving this is through proprietary dye chemistry that generates robust fluorescent signals upon enzymatic cleavage, enabling detection of enzyme activity at picomolar concentrations. Multiple wavelength options (blue, green, red, and NIR) allow flexible multiplexing with other biochemical assays. For more information about this group of assays, see our Enzyme Activity Assays & Substrates catalog.

Amplite® metabolite quantitation kits employ coupled enzymatic reactions to convert target metabolites into detectable fluorescent signals, enabling accurate quantitation of metabolic analytes in biochemical samples. These assays cover key pathways including carbohydrate metabolism, lipid metabolism, and redox coenzyme cycling, and are optimized for serum, plasma, cell lysates, tissue homogenates, and other biological matrices with minimal sample preparation.

ROS detection assays measure hydrogen peroxide, superoxide, nitric oxide, peroxynitrite, and lipid peroxidation markers in cell-free systems. Amplite® cell-free kits provide ultrasensitive quantitation of H₂O₂ and other oxidants in solution assays. These assays are essential for oxidative stress research, antioxidant screening, and studying disease mechanisms linked to redox imbalance. For complete oxidative stress solutions, see our Reactive Oxygen Signaling Species (ROS) catalog.

Helixyte™, StrandBrite™, and Amplite® kits enable fluorimetric quantitation of nucleic acids, proteins, and metal ions with sensitivity suitable for precious samples. DNA and RNA quantitation kits provide accurate measurements for NGS library preparation, PCR template quantitation, and sample quality control. Protein and bioconjugate quantitation assays complement the portfolio for comprehensive biomolecule characterization in research workflows.