AAT Bioquest provides a comprehensive portfolio of enzyme activity assays and substrates for sensitive, quantitative detection of enzyme function in biochemical research and drug discovery applications. Our portfolio includes both complete enzyme activity assay kits and standalone fluorogenic or chromogenic substrates, enabling quantitative measurement of enzyme kinetics across phosphatases, proteases, oxidases, kinases, and other enzyme classes using flexible detection formats.

Amplite® — Kits with high-sensitivity fluorimetric and colorimetric detection
Tide Fluor™/Tide Quencher™ — Optimized FRET pairs for protease substrates and molecular beacons
PhosphoWorks™ — ATP, ADP, and phosphate detection for kinase activity and metabolic studies

Peroxidase assays and substrates enable detection of horseradish peroxidase activity using fluorogenic or colorimetric substrates, as well as signal amplification reagents commonly used for enzyme-labeled detection applications. HRP is widely used as an enzyme label in ELISA, Western blotting, and immunohistochemistry applications.

Alkaline phosphatase assays enable detection of ALP activity in serum, cell lysates, and tissue samples. ALP is an important biomarker for bone metabolism, liver function, and various disease states.

β-Galactosidase substrates support detection of reporter enzyme activity in lacZ-based gene expression assays, as well as measurement of endogenous β-galactosidase activity in biochemical and cellular samples.

β-Glucuronidase substrates enable detection of GUS reporter gene activity and endogenous glucuronidase in drug metabolism studies.

Caspase substrates and inhibitors provide tools for detecting apoptosis through caspase activity measurement. Our portfolio covers initiator caspases (8, 9, 10) and executioner caspases (3, 6, 7).

Protease substrates utilize FRET-based detection for measuring MMP, cathepsin, and other protease activities. These substrates are essential for cancer research, inflammation studies, and drug screening.

Protein phosphatase assays detect activity of PP1, PP2A, calcineurin, and other serine/threonine phosphatases involved in cellular signaling.

Phosphodiesterase assays measure PDE activity for cAMP and cGMP signaling research and drug discovery targeting PDE inhibitors.

Additional hydrolase assays cover esterases, lipases, sulfatases, and other hydrolytic enzymes.

Protein kinase assays measure kinase activity through ATP consumption or phosphopeptide product detection.

Luciferase substrates provide bioluminescent detection of firefly and Renilla luciferase reporter genes.

Oxidase assays measure activity of glucose oxidase, monoamine oxidase, xanthine oxidase, and related enzymes through coupled detection of hydrogen peroxide generated during enzymatic reactions.

Reductase assays measure nitroreductase, quinone reductase, and related enzyme activities through probe reduction or conversion-based detection methods used in drug metabolism and cellular response studies.

HDAC assays provide fluorogenic detection of histone deacetylase activity for epigenetics and cancer research.