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Bioconjugation and Tags
Bioconjugation is the process of linking molecules together, at least one of which is a biomolecule, to create probes, detection reagents, and research tools. AAT Bioquest provides comprehensive solutions for conjugating antibodies, proteins, and other biomolecules with fluorescent dyes, enzymes, affinity tags, and additional labels.
Our Bioconjugation Technologies
ReadiLink™ — High-yield labeling with small molecules such as fluorescent dyes, no purification required
Buccutite™ — Proprietary chemistry optimized for labeling with proteins like HRP, PE, and APC
ReadiUse™ — One-step labeling with preactivated dyes and tags, ideal for quick conjugation workflows
Buccutite™ — Proprietary chemistry optimized for labeling with proteins like HRP, PE, and APC
ReadiUse™ — One-step labeling with preactivated dyes and tags, ideal for quick conjugation workflows
Looking for custom conjugation? Explore our Antibody Conjugation and Labeling Services or browse Custom Labeling Options.
Browse Bioconjugation and Tags
Small Fluorescent Labeling Dyes
Small-molecule fluorescent dyes enable researchers to label proteins, antibodies, nucleic acids, and other biomolecules for detection across virtually any fluorescence-based platform. Our portfolio spans the UV to infrared spectrum, featuring proprietary dye families engineered for superior brightness, photostability, and water solubility.
- iFluor® dyes deliver superior brightness and photostability compared to Alexa Fluor® and DyLight™
- mFluor™ dyes are optimized specifically for multicolor flow cytometry panels
- XFD dyes and AATOM™ dyes offer cost-effective alternatives to other commercial dyes
- Classic reactive dye families including cyanines, fluoresceins, rhodamines, and coumarins
- Available in NHS ester, maleimide, azide, alkyne, and other reactive forms
FRET Building Blocks
Förster Resonance Energy Transfer (FRET) enables distance-dependent detection of molecular interactions through energy transfer between donor and acceptor fluorophores. Our selection includes optimized FRET pairs, dark quenchers, and time-resolved fluorescence reagents for protease assays, molecular beacons, and proximity-based detection.
- Tide Fluor™ dyes deliver bright, photostable donor fluorescence for FRET applications
- Tide Quencher™ dyes provide broad spectral coverage for efficient energy quenching
- Classic dark quenchers including DABCYL and AzoDye variants for probe design
- trFluor™ reagents enable time-resolved FRET without enhancer solutions
- Compatible with peptide synthesis, oligonucleotide labeling, and protein conjugation
Fluorescent Labeling Proteins
Phycobiliproteins, including phycoerythrin (PE), allophycocyanin (APC), and peridinin-chlorophyll-protein (PerCP), offer exceptional brightness for flow cytometry and immunoassays. Tandem conjugates couple these proteins with small molecule acceptor dyes, expanding spectral options for multicolor panel design.
- PE tandems provide orange to far-red emission options for 488 nm or 561 nm excitation
- APC tandems extend red laser detection into the far-red and near-infrared
- PerCP tandems offer additional flexibility for violet and blue laser configurations
- Pre-activated proteins and conjugation kits available for custom antibody labeling
- Optimized for minimal spectral spillover in multicolor flow cytometry
Nanoparticles
Nanoparticle reagents include latex bead conjugates and quantitation tools for nanoparticle characterization. These macromolecular probes are widely used for cellular tracing, endocytosis studies, and in vivo imaging applications.
- Latex bead conjugates for endocytosis and phagocytosis tracking
- Beadlite™ quantitation kits for measuring amino, maleimide, thiol, and biotin groups
- Multiple molecular weights available for size-dependent studies
- Suitable for microinjection, bulk loading, and nanoparticle surface characterization
Enzyme Tags
Enzyme-based detection systems provide signal amplification through catalytic turnover, making them ideal for low-abundance target detection. Horseradish peroxidase (HRP) and alkaline phosphatase (ALP) remain the gold standard for immunoassays, Western blotting, and immunohistochemistry.
- HRP conjugates for chromogenic, chemiluminescent, and fluorescent detection
- ALP conjugates for enhanced chemiluminescence and colorimetric applications
- Buccutite™ conjugation kits for custom antibody-enzyme labeling
- Compatible with standard ELISA, Western blot, and IHC workflows
- Optimized for high specific activity and low background
ReadiLink™ Protein Labeling Kits
ReadiLink™ kits streamline antibody and protein labeling with optimized protocols that deliver consistent results in under two hours. These single-use kits eliminate guesswork in dye-to-protein ratio optimization and purification.
- Pre-optimized labeling chemistry for microscale to production scale
- No column purification required for efficient antibody recovery
- Consistent degree of labeling across different antibody lots
- Available for iFluor®, XFD, PE, APC, PerCP, and tandem dyes
- Suitable for IgG, IgM, and other immunoglobulin classes
Buccutite™ Protein Crosslinking Kits
Buccutite™ kits enable efficient protein-to-protein and antibody-to-label conjugation while preserving biological activity. These kits are ideal for generating antibody-fluorophore conjugates, antibody-enzyme conjugates, and multi-protein complexes.
- Gentle conjugation chemistry preserves antigen binding and protein function
- Optimized for antibody labeling with PE, APC, PerCP, and tandem dyes
- HRP and ALP conjugation kits for enzyme-linked detection
- Scalable from analytical (25 µg) to production quantities (5 mg)
- Rapid protocols with minimal optimization required
Luminescent Tag Molecules
Luminescent tags—including acridinium esters and related chemiluminescent labels—provide sensitive detection without external excitation, eliminating background fluorescence for ultrasensitive immunoassays.
- Acridinium esters and DMAE derivatives for flash chemiluminescence
- Lumiwox™ acridinium NHS ester for enhanced signal stability
- Luminol for peroxidase-based chemiluminescent detection
- Available in NHS ester, maleimide, azide, alkyne, and biotin forms
- Suitable for automated immunoassay platforms and high-sensitivity applications
Other Tag Molecules
Specialty tags for affinity purification, protein detection, and custom labeling applications.
- Polyhistidine (6XHis and 10XHis) tags for affinity purification and detection
- Available with NHS ester, maleimide, azide, and alkyne reactive groups
- Useful for protein engineering and recombinant protein workflows
- Compatible with standard Ni-NTA and metal affinity chromatography
- Building blocks for custom probe and conjugate synthesis
This document (01.0348.251203r1) was last updated on Fri Feb 13 2026. All trademarks and registered trademarks mentioned herein are the property of their respective owners.