5-TAMRA alkyne

5-TAMRA alkyne is an excellent azide-reactive fluorescent reagent for labeling alkyne-containing biological molecules through the well known click chemistry. It has the spectral properties identical to those of TRITC conjugates, thus all the instrument settings of the TRITC conjugates would be applicable to the applications of 5-TAMRA alkyne conjugates.

Protocol

COA

Catalog	Size	Price	Quantity
487	5 mg	Price
961	50 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 5-TAMRA alkyne to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	213.895 µL	1.069 mL	2.139 mL	10.695 mL	21.389 mL
5 mM	42.779 µL	213.895 µL	427.789 µL	2.139 mL	4.278 mL
10 mM	21.389 µL	106.947 µL	213.895 µL	1.069 mL	2.139 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	467.52 g/mol	=	mL	x	mM	=	mmol

Citations

View all 4 citations: Citation Explorer

Suppression of ATG4B by copper inhibits autophagy and involves in Mallory body formation

Authors:

Xia, Fan and Fu, Yuanyuan and Xie, Huazhong and Chen, Yuxin and Fang, Dongmei and Zhang, Wei and Liu, Peiqing and Li, Min

Journal:

Redox biology (2022): 102284

Design and Characterization of Two Bifunctional Cryptophane A-Based Host Molecules for Xenon Magnetic Resonance Imaging Applications

Authors:

Rossella, Federica and Rose, Honor May and Witte, Christopher and Jayapaul, Jabadurai and Schröder, Leif

Journal:

ChemPlusChem (2014): 1463--1471

Human erythrocytes as drug carriers: loading efficiency and side effects of hypotonic dialysis, chlorpromazine treatment and fusion with liposomes

Authors:

Favretto, ME and Cluitmans, JCA and Bosman, GJCGM and Brock, R

Journal:

Journal of Controlled Release (2013): 343--351

Advances in Quantitative FRET-Based Methods for Studying Nucleic Acids

Authors:

Preus, Søren and Wilhelmsson, L Marcus

Journal:

ChemBioChem (2012): 1990--2001

References

View all 96 references: Citation Explorer

Metal-enhanced fluorescence-based RNA sensing

Authors:

Aslan K, Huang J, Wilson GM, Geddes CD.

Journal:

J Am Chem Soc (2006): 4206

G Quadruplex-Based FRET Probes with the Thrombin-Binding Aptamer (TBA) Sequence Designed for the Efficient Fluorometric Detection of the Potassium Ion

Authors:

Nagatoishi S, Nojima T, Galezowska E, Juskowiak B, Takenaka S.

Journal:

Chembiochem (2006): 1730

A targeted protease substrate for a quantitative determination of protease activities in the endolysosomal pathway

Authors:

Fischer R, Bachle D, Fotin-Mleczek M, Jung G, Kalbacher H, Brock R.

Journal:

Chembiochem (2006): 1428

Electrostatic-gated transport in chemically modified glass nanopore electrodes

Authors:

Wang G, Zhang B, Wayment JR, Harris JM, White HS.

Journal:

J Am Chem Soc (2006): 7679

Fluorescence anisotropy and FRET studies of G-quadruplex formation in presence of different cations

Authors:

Juskowiak B, Galezowska E, Zawadzka A, Gluszynska A, Takenaka S.

Journal:

Spectrochim Acta A Mol Biomol Spectrosc (2006): 835

Physical properties

Molecular weight	467.52
Solvent	DMSO

Spectral properties

Correction factor (260 nm)	0.32
Correction factor (280 nm)	0.178
Extinction coefficient (cm ^-1 M ^-1)	90000
Excitation (nm)	552
Emission (nm)	578

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

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Page updated on October 12, 2025