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6-VIC, SE [6-VIC NHS ester]
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Application notes
6-VIC, SE is a popular amino-reactive fluorescent probe that is widely used in nucleic acid sequencing and related research. It is also often used for labeling oligos.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of 6-VIC, SE [6-VIC NHS ester] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 153.182 µL | 765.908 µL | 1.532 mL | 7.659 mL | 15.318 mL |
| 5 mM | 30.636 µL | 153.182 µL | 306.363 µL | 1.532 mL | 3.064 mL |
| 10 mM | 15.318 µL | 76.591 µL | 153.182 µL | 765.908 µL | 1.532 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Correction Factor (280 nm) |
| 6-FAM, SE [6-Carboxyfluorescein, succinimidyl ester] *CAS 92557-81-8* | 493 | 517 | 83000 | 0.178 |
| 6-HEX, SE [6-Carboxy-2',4,4',5',7,7'-hexachlorofluorescein, succinimidyl ester] *Single Isomer* | 533 | 559 | 73000 | 0.15 |
| 6-JOE, SE [6-Carboxy-4',5'-dichloro-2',7'-dimethoxyfluorescein, succinimidyl ester] *CAS#: 113394-23-3* | 520 | 545 | 750001 | - |
| 6-TET, SE [6-Carboxy-2',4,7',7-tetrachlorofluorescein, succinimidyl ester] | 521 | 542 | 73000 | 0.13 |
| 6-CR6G, SE [6-Carboxyrhodamine 6G, succinimidyl ester] *Single isomer* | 522 | 546 | 94000 | 0.214 |
| 6-TAMRA, SE [6-Carboxytetramethylrhodamine, succinimidyl ester] *CAS#: 150810-69-8* | 552 | 578 | 90000 | 0.178 |
| 6-ROX, SE [6-Carboxy-X-rhodamine, succinimidyl ester] *CAS#: 216699-36-4* | 578 | 604 | 82000 | 0.168 |
| 6-ROXtra™ SE *Superior 6-ROX Replacement* | 578 | 595 | - | - |
| 6-NED, SE [6-NED NHS ester] | 545 | 567 | 740001 | - |
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