BOC-Leu-Gly-Arg-Aminoluciferin

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Chemical structure for BOC-Leu-Gly-Arg-Aminoluciferin
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Unit Size: Cat No: Price (USD): Qty:
1 mg 13001 $495


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Overview

Ex/Em (nm)None/560
MW819.87
SolventDMSO
Storage Freeze (<-15 °C)
Minimize light exposure
Category Enzyme Detection
Peptidases and Proteases
Related Cell Apoptosis
Secondary Reagents
This aminoluciferin-based probe is a luminogenic substrate for complement component C3/C5 convertases, coagulation factor Xa, and soybean trypsin-like enzyme. It is also hydrolyzed by macropain, a high molecular weight thiol proteinase from human erythrocytes. The protease-released D-aminoluciferin can be detected using our luciferase assay kits. BOC-Leu-Gly-Arg-Aminoluciferin is much more sensitive than Boc-Leu-Gly-Arg-AMC, the widely used fluorogenic AMC substrate.




Calculators
Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of BOC-Leu-Gly-Arg-Aminoluciferin to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.



Molarity calculator

Table 2. Enter any two values (mass, volume, concentration) to calculate the third.

Mass Molecular weight Volume Concentration Moles
/ = x =
 






References & Citations

Endotoxin assay by bioluminescence using mutant firefly luciferase
Authors: Kenichi Noda, Hitoshi Goto, Yuji Murakami, Abo Bakr F Ahmed, Akio Kuroda
Journal: Analytical biochemistry (2010): 152--155






Additional Documents

 
Safety Data Sheet (SDS)


Catalogs
1. Enzyme Probes & Assay Kits

Certificate of Analysis