Bucculite™ XdU Cell Proliferation Fluorescence Imaging Kit provides a fast and mild method for analyzing active DNA synthesis in proliferating cells by fluorescence microscopy. The assay detects XdU incorporation into newly synthesized DNA, enabling visualization of cells undergoing S-phase progression without the harsh DNA denaturation steps required for traditional BrdU-based detection.

The kit is designed for fluorescence imaging workflows and uses chemistry developed to minimize copper-related quenching of fluorescent proteins and phycobiliprotein-based fluorophores. The deep red fluorescent signal is compatible with standard Cy5-compatible filter settings, making it suitable for common fluorescence microscopy platforms. The streamlined workflow avoids lengthy antibody incubation steps and supports completion of labeling and detection in as little as 60 minutes. Bucculite™ XdU Cell Proliferation Fluorescence Imaging Kit is suitable for cell proliferation imaging, S-phase analysis, compound response studies, and microscopy-based cell biology applications.