logo
Products
Technologies
Applications
Services
Resources
Selection Guides
About
Calbryte™-520XL AM

Calbryte™-520XL, AM is a new fluorescent and cell-permeable calcium indicator with extremely low affinity. Like other dye AM cell loading, Calbryte™-520XL AM ester is non-fluorescent, and once it gets inside the cell, it is hydrolyzed by intracellular esterase and gets activated. The activated indicator is a polar molecule that can no longer freely diffuse through the cell membrane, essentially trapped inside cells. Calbryte™-520XL has a low affinity for calcium ions with a Kd ~300 uM, similar to the well-known Rhod 5N, but it is much more stable. Calbryte™-520XL produces a bright fluorescence signal in the presence of calcium ions in high concentration. It has an excitation and emission wavelength identical to Fluo-4. Thus, the same Fluo-4 assay settings can be readily applied to Calbryte™-520XL-based calcium assays. Calbryte™-520XL is an excellent alternative to Rhod-5N. We also offer Calbryte™-520XL, potassium salt (#20645), Calbryte™-520XL-dextran (#20648), Calbryte™-520XL azide (#20643) that can be readily conjugated to a carrier through the well-known click chemistry.

Ca2+ dependent fluorescence emission of Calbryte™-520XL indicator (Ex/Em = 490/525 nm).
Ca2+ dependent fluorescence emission of Calbryte™-520XL indicator (Ex/Em = 490/525 nm).
CatalogSize
Price
Quantity
2064610x50 ug
Price
 
Physical properties

Dissociation constant (Kd, nM)300000
Molecular weight952.79
SolventDMSO
Spectral properties

Excitation (nm)493
Emission (nm)515
Quantum yield
0.75
1
Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200
Instrument settings

Fluorescence microscope
ExcitationFITC
EmissionFITC
Recommended plateBlack wall/clear bottom

Fluorescence microplate reader
Excitation490
Emission525
Cutoff515
Recommended plateBlack wall/clear bottom
Instrument specification(s)Bottom read mode/Programmable liquid handling
Contact us

Telephone
Fax
Emailsales@aatbio.com
InternationalSee distributors
Bulk requestInquire
Custom sizeInquire
Technical SupportContact us
Request quotationRequest
Purchase orderSend to sales@aatbio.com
ShippingStandard overnight for United States, inquire for international
Page updated on October 13, 2025