Cell Explorer™ Live Cell Labeling Kit *Blue Fluorescence*

Cell Explorer™ Live Cell Labeling Kit

Blue Fluorescence

Our Cell Explorer™ fluorescence imaging kits are a set of tools for labeling cells for fluorescence microscopic investigations of cellular functions. The effective labeling of cells provides a powerful method for studying cellular events in a spatial and temporal context. This particular kit is designed to uniformly label live cells in blue fluorescence. The kit uses a proprietary dye that gets enhanced fluorescence upon entering into live cells. The dye is a hydrophobic compound that easily permeates intact live cells. The hydrolysis of the weakly fluorescent substrate by intracellular esterases generates a strongly fluorescent hydrophilic product that is well-retained in the cell cytoplasm. Cells grown in black-walled plates can be stained and quantified in less than two hours. It can be readily adapted for high-throughput assays in a wide variety of fluorescence platforms such as microplate assays, immunocytochemistry and flow cytometry. It is useful for a variety of studies, including cell adhesion, chemotaxis, multidrug resistance, cell viability, apoptosis and cytotoxicity. The kit provides all the essential components with an optimized cell-labeling protocol.

Protocol

SDS

Catalog	Size	Price	Quantity
22606	200 Tests	Price

Citations

View all 6 citations: Citation Explorer

Development of a novel senolysis approach targeting the senescent fibroblast marker HTR2A via antibody-dependent cellular cytotoxicity.

Authors:

Takaya, Kento and Asou, Toru and Kishi, Kazuo

Journal:

Rejuvenation Research (2023)

The preparation of hollow mesoporous bioglass nanoparticles with excellent drug delivery capacity for bone tissue regeneration

Authors:

Wang, Yudong and Pan, Haobo and Chen, Xiaofeng

Journal:

Frontiers in chemistry (2019): 283

Autophagy proteins are not universally required for phagosome maturation

Authors:

Cemma, Marija and Grinstein, Sergio and Brumell, John H

Journal:

Autophagy (2016): 1440--1446

Size control and biological properties of monodispersed mesoporous bioactive glass sub-micron spheres

Authors:

Hu, Qing and Li, Yuli and Miao, Guohou and Zhao, Naru and Chen, Xiaofeng

Journal:

Rsc Advances (2014): 22678--22687

Differential detection of tumor cells using a combination of cell rolling, multivalent binding, and multiple antibodies

Authors:

Myung, Ja Hye and Gajjar, Khyati A and Chen, Jihua and Molokie, Robert E and Hong, Seungpyo

Journal:

Analytical chemistry (2014): 6088--6094

References

View all 26 references: Citation Explorer

Requirements, features, and performance of high content screening platforms

Authors:

Gough AH, Johnston PA.

Journal:

Methods Mol Biol (2007): 41

A pharmaceutical company user's perspective on the potential of high content screening in drug discovery

Authors:

Hoffman AF, Garippa RJ.

Journal:

Methods Mol Biol (2007): 19

Optimizing the integration of immunoreagents and fluorescent probes for multiplexed high content screening assays

Authors:

Giuliano KA., undefined

Journal:

Methods Mol Biol (2007): 189

Past, present, and future of high content screening and the field of cellomics

Authors:

Taylor DL., undefined

Journal:

Methods Mol Biol (2007): 3

High concordance of drug-induced human hepatotoxicity with in vitro cytotoxicity measured in a novel cell-based model using high content screening

Authors:

O'Brien P J, Irwin W, Diaz D, Howard-Cofield E, Krejsa CM, Slaughter MR, Gao B, Kaludercic N, Angeline A, Bernardi P, Brain P, Hougham C.

Journal:

Arch Toxicol (2006): 580

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Fluorescence microscope
Excitation	DAPI filter set
Emission	DAPI filter set
Recommended plate	Black wall/clear bottom

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Page updated on July 15, 2026

Component A: Calcein UltraBlue™	1 vial
Component B: HHBS (Hanks' buffer with 20 mM Hepes)	1 bottle (100 mL)