Cell Explorer™ Live Cell Labeling Kit *Blue Fluorescence*

Cell Explorer™ Live Cell Labeling Kit

Blue Fluorescence

Cell Explorer™ Live Cell Labeling Kit is a fluorescence-based kit for labeling living cells with a blue fluorescent product generated by intracellular esterase activity.

Esterase-activated labeling: Intracellular esterases convert the weakly fluorescent substrate into a strongly fluorescent product
Cytoplasmic retention: Generates a hydrophilic fluorescent product that is retained in the cell cytoplasm
Blue fluorescence detection: Supports fluorescence microscopy using a DAPI-compatible filter set
Applications: Suitable for live-cell labeling, cell adhesion and chemotaxis studies, and fluorescence-based cell assays

Image of HeLa cells stained with Cell Explorer™ Live Cell Labeling Kit *Blue Fluorescence* (Cat#22606) in a Costar black wall/clear bottom 96-well plate. Cells were stained with Calcein UltraBlue™ for 30 minutes at 37 <sup>o</sup>C. Images were aquired using a fluorescence microscope using DAPI filter.

Protocol

SDS

Catalog	Size	Price	Quantity
22606	200 Tests	Price

Overview

Cell Explorer™ Live Cell Labeling Kit is designed for blue fluorescent labeling of living cells using Calcein UltraBlue™. The cell-permeable, weakly fluorescent substrate enters cells, where intracellular esterases hydrolyze it to produce a strongly fluorescent, hydrophilic product. Intracellular retention of this product enables visualization of the labeled cell cytoplasm.

The kit includes Calcein UltraBlue™ and HHBS buffer, with sufficient reagents for 200 tests. Blue fluorescence can be detected by fluorescence microscopy using a DAPI-compatible filter set, and the labeling approach can be adapted to compatible microplate and flow cytometry platforms. Cell Explorer™ Live Cell Labeling Kit supports live-cell imaging, cell adhesion and chemotaxis studies, and fluorescence-based cell assays.

Citations

View all 6 citations: Citation Explorer

Development of a novel senolysis approach targeting the senescent fibroblast marker HTR2A via antibody-dependent cellular cytotoxicity.

Authors:

Takaya, Kento and Asou, Toru and Kishi, Kazuo

Journal:

Rejuvenation Research (2023)

The preparation of hollow mesoporous bioglass nanoparticles with excellent drug delivery capacity for bone tissue regeneration

Authors:

Wang, Yudong and Pan, Haobo and Chen, Xiaofeng

Journal:

Frontiers in chemistry (2019): 283

Autophagy proteins are not universally required for phagosome maturation

Authors:

Cemma, Marija and Grinstein, Sergio and Brumell, John H

Journal:

Autophagy (2016): 1440--1446

Size control and biological properties of monodispersed mesoporous bioactive glass sub-micron spheres

Authors:

Hu, Qing and Li, Yuli and Miao, Guohou and Zhao, Naru and Chen, Xiaofeng

Journal:

Rsc Advances (2014): 22678--22687

Differential detection of tumor cells using a combination of cell rolling, multivalent binding, and multiple antibodies

Authors:

Myung, Ja Hye and Gajjar, Khyati A and Chen, Jihua and Molokie, Robert E and Hong, Seungpyo

Journal:

Analytical chemistry (2014): 6088--6094

References

View all 26 references: Citation Explorer

Requirements, features, and performance of high content screening platforms

Authors:

Gough AH, Johnston PA.

Journal:

Methods Mol Biol (2007): 41

A pharmaceutical company user's perspective on the potential of high content screening in drug discovery

Authors:

Hoffman AF, Garippa RJ.

Journal:

Methods Mol Biol (2007): 19

Optimizing the integration of immunoreagents and fluorescent probes for multiplexed high content screening assays

Authors:

Giuliano KA., undefined

Journal:

Methods Mol Biol (2007): 189

Past, present, and future of high content screening and the field of cellomics

Authors:

Taylor DL., undefined

Journal:

Methods Mol Biol (2007): 3

High concordance of drug-induced human hepatotoxicity with in vitro cytotoxicity measured in a novel cell-based model using high content screening

Authors:

O'Brien P J, Irwin W, Diaz D, Howard-Cofield E, Krejsa CM, Slaughter MR, Gao B, Kaludercic N, Angeline A, Bernardi P, Brain P, Hougham C.

Journal:

Arch Toxicol (2006): 580

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Fluorescence microscope
Excitation	DAPI filter set
Emission	DAPI filter set
Recommended plate	Black wall/clear bottom

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 27, 2026

Component A: Calcein UltraBlue™	1 vial
Component B: HHBS (Hanks' buffer with 20 mM Hepes)	1 bottle (100 mL)