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Cell Explorer™ Live Cell Labeling Kit
Blue Fluorescence
Our Cell Explorer™ fluorescence imaging kits are a set of tools for labeling cells for fluorescence microscopic investigations of cellular functions. The effective labeling of cells provides a powerful method for studying cellular events in a spatial and temporal context. This particular kit is designed to uniformly label live cells in blue fluorescence. The kit uses a proprietary dye that gets enhanced fluorescence upon entering into live cells. The dye is a hydrophobic compound that easily permeates intact live cells. The hydrolysis of the weakly fluorescent substrate by intracellular esterases generates a strongly fluorescent hydrophilic product that is well-retained in the cell cytoplasm. Cells grown in black-walled plates can be stained and quantified in less than two hours. It can be readily adapted for high-throughput assays in a wide variety of fluorescence platforms such as microplate assays, immunocytochemistry and flow cytometry. It is useful for a variety of studies, including cell adhesion, chemotaxis, multidrug resistance, cell viability, apoptosis and cytotoxicity. The kit provides all the essential components with an optimized cell-labeling protocol.
Image of HeLa cells stained with Cell Explorer&trade; Live Cell Labeling Kit *Blue Fluorescence* (Cat#22606) in a Costar black wall/clear bottom 96-well plate. Cells were stained with&nbsp;Calcein UltraBlue&trade; for 30 minutes at&nbsp;37 <sup>o</sup>C. Images were aquired using a fluorescence microscope using DAPI filter.
Image of HeLa cells stained with Cell Explorer&trade; Live Cell Labeling Kit *Blue Fluorescence* (Cat#22606) in a Costar black wall/clear bottom 96-well plate. Cells were stained with&nbsp;Calcein UltraBlue&trade; for 30 minutes at&nbsp;37 <sup>o</sup>C. Images were aquired using a fluorescence microscope using DAPI filter.
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
22606200 Tests
Price
 
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12352200
Instrument settings
Fluorescence microscope
ExcitationDAPI filter set
EmissionDAPI filter set
Recommended plateBlack wall/clear bottom
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Page updated on September 25, 2025