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Cell Explorer™ Live Cell Labeling Kit *Green Fluorescence with 405 nm Excitation*

Image of CPA cells in 96-well Costar black wall/clear bottom plate stained with Cell Explorer™ Live Cell Labeling Kit *Green Fluorescence with 405 nm Excitation*(Cat#22615). Cells were stained using CytoCalcein™ Violet 500 for 30 minutes. Image was aquired with fluorescence microscope using Violet filter.
Image of CPA cells in 96-well Costar black wall/clear bottom plate stained with Cell Explorer™ Live Cell Labeling Kit *Green Fluorescence with 405 nm Excitation*(Cat#22615). Cells were stained using CytoCalcein™ Violet 500 for 30 minutes. Image was aquired with fluorescence microscope using Violet filter.
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Catalog Number22615
Unit Size
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Additional ordering information
InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Spectral properties
Excitation (nm)420
Emission (nm)505
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22


Excitation (nm)
Emission (nm)
Our Cell Explorer™ fluorescence imaging kits are a set of tools for labeling cells for fluorescence microscopic investigations of cellular functions. The effective labeling of cells provides a powerful method for studying cellular events in a spatial and temporal context. This particular kit is designed to uniformly label live cells for the flow cytometric analysis of live cells with the violet laser (405 nm excitation). The kit uses a proprietary dye that gets enhanced fluorescence upon entering into live cells. The dye is a hydrophobic compound that easily permeates intact live cells. The hydrolysis of the weakly fluorescent substrate by intracellular esterases generates a strongly fluorescent hydrophilic product that is well-retained in the cell cytoplasm. It can be readily adapted for flow cytometry applications. The fluorescent dye used in the kit is well excited with the violet laser (405 nm excitation) to fluorescence at ~510 nm. The kit provides all the essential components with an optimized cell-labeling protocol. It is useful for a variety of studies, including cell adhesion, chemotaxis, multidrug resistance, cell viability, apoptosis and cytotoxicity.


Flow cytometer

Excitation405 nm laser
Emission525/40 nm filter
Instrument specification(s)AmCyan channel

Fluorescence microscope

ExcitationViolet filter set
EmissionViolet filter set
Recommended plateBlack wall/clear bottom


Component A: CytoCalcein™ Violet 5002 vials
Component B: HHBS 1 bottle (100 mL)

Example protocol


Protocol summary

  1. Prepare cells in growth medium
  2. Remove growth medium
  3. Add CytoCalcein™ Violet 500 working solution (100 µL/well for a 96-well plate or 25 µL/well for a 384-well plate)
  4. Incubate the cells at 37°C for 30 minutes to 1 hour
  5. Wash the cells
  6. Examine the specimen under fluorescence microscope with Violet filter set or flow cytometer with 525/40 nm filter (AmCyan channel)

Important notes
Thaw all the components at room temperature before opening.


Unless otherwise noted, all unused stock solutions should be divided into single-use aliquots and stored at -20 °C after preparation. Avoid repeated freeze-thaw cycles.

1. CytoCalcein™ Violet 500 stock solution:
Add 20 µL of DMSO into the vial of CytoCalcein™ Violet 500 (Component A) and mix well to make CytoCalcein™ Violet 500 stock solution. Note: 20 µL of CytoCalcein™ Violet 500 stock solution is enough for 1 plate. Note: Unused CytoCalcein™ Violet 500 stock solution can be aliquoted and stored at < -20 oC for more than one month if the tubes are sealed tightly. Avoid repeated freeze-thaw cycles and protect it from light.


Add 20 µL of CytoCalcein™ Violet 500 stock solution into 10 mL of HHBS (Component B) and mix well to make CytoCalcein™ Violet 500 working solution.

For guidelines on cell sample preparation, please visit


  1. Remove the growth medium.

  2. Add 100 µL/well (96-well plate) or 25 µL/well (384-well plate) CytoCalcein™ Violet 500 working solution into the cell plate.

  3. Incubate the cells in a 37°C, 5% CO2 incubator for 30 minutes to 1 hour.

  4. Remove the CytoCalcein™ Violet 500 working solution from the cells, wash the cells with HHBS (Component B) for 2 to 3 times, and replace with HHBS.

  5. Analyze the cells using a fluorescence microscope  with Violet filter set or flow cytometer with 525/40 nm filter (AmCyan channel). Note: Alternatively, cells can be fixed at this point for later imaging (fluorescence intensity might be decreased upon fixation).


Open in Advanced Spectrum Viewer

Spectral properties

Excitation (nm)420
Emission (nm)505


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Journal: Autophagy (2016): 1440--1446
Differential detection of tumor cells using a combination of cell rolling, multivalent binding, and multiple antibodies
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Journal: Analytical chemistry (2014): 6088--6094
Size control and biological properties of monodispersed mesoporous bioactive glass sub-micron spheres
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Versatile fabrication of nanoscale sol--gel bioactive glass particles for efficient bone tissue regeneration
Authors: Lei, Bo and Chen, Xiaofeng and Han, Xue and Zhou, Jiaan
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Authors: Gough AH, Johnston PA.
Journal: Methods Mol Biol (2007): 41
A pharmaceutical company user's perspective on the potential of high content screening in drug discovery
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