Name: Cell Explorer™ Live Cell Labeling Kit *Green Fluorescence*
Brand: AAT Bioquest
SKU: 22607
Price: 237 USD
Availability: InStock

Cell Explorer™ Live Cell Labeling Kit

Green Fluorescence

Our Cell Explorer™ fluorescence imaging kits are a set of tools for labeling cells for fluorescence microscopic investigations of cellular functions. The effective labeling of cells provides a powerful method for studying cellular events in a spatial and temporal context. This particular kit is designed to uniformly label live cells in green fluorescence. The kit uses non-fluorescent Calcein Green™ that becomes strongly fluorescent upon entering into live cells. Calcein Green™ is a hydrophobic compound that easily permeates intact live cells. The hydrolysis of the non-fluorescent Calcein Green™ by intracellular esterases generates the strongly fluorescent hydrophilic Calcein Green™ fluorophore that is well-retained in the cell cytoplasm. Cells grown in black-walled plates can be stained and quantified in less than two hours. It can be readily adapted for high-throughput assays in a wide variety of fluorescence platforms such as microplate assays, immunocytochemistry and flow cytometry. It is useful for a variety of studies, including cell adhesion, chemotaxis, multidrug resistance, cell viability, apoptosis and cytotoxicity. The kit provides all the essential components with an optimized cell-labeling protocol.

Protocol

SDS

Catalog	Size	Price	Quantity
22607	200 Tests	Price

Citations

View all 5 citations: Citation Explorer

Development of a novel senolysis approach targeting the senescent fibroblast marker HTR2A via antibody-dependent cellular cytotoxicity.

Authors:

Takaya, Kento and Asou, Toru and Kishi, Kazuo

Journal:

Rejuvenation Research (2023)

Autophagy proteins are not universally required for phagosome maturation

Authors:

Cemma, Marija and Grinstein, Sergio and Brumell, John H

Journal:

Autophagy (2016): 1440--1446

Size control and biological properties of monodispersed mesoporous bioactive glass sub-micron spheres

Authors:

Hu, Qing and Li, Yuli and Miao, Guohou and Zhao, Naru and Chen, Xiaofeng

Journal:

Rsc Advances (2014): 22678--22687

Differential detection of tumor cells using a combination of cell rolling, multivalent binding, and multiple antibodies

Authors:

Myung, Ja Hye and Gajjar, Khyati A and Chen, Jihua and Molokie, Robert E and Hong, Seungpyo

Journal:

Analytical chemistry (2014): 6088--6094

Versatile fabrication of nanoscale sol--gel bioactive glass particles for efficient bone tissue regeneration

Authors:

Lei, Bo and Chen, Xiaofeng and Han, Xue and Zhou, Jiaan

Journal:

Journal of Materials Chemistry (2012): 16906--16913

References

View all 26 references: Citation Explorer

Requirements, features, and performance of high content screening platforms

Authors:

Gough AH, Johnston PA.

Journal:

Methods Mol Biol (2007): 41

A pharmaceutical company user's perspective on the potential of high content screening in drug discovery

Authors:

Hoffman AF, Garippa RJ.

Journal:

Methods Mol Biol (2007): 19

Optimizing the integration of immunoreagents and fluorescent probes for multiplexed high content screening assays

Authors:

Giuliano KA., undefined

Journal:

Methods Mol Biol (2007): 189

Past, present, and future of high content screening and the field of cellomics

Authors:

Taylor DL., undefined

Journal:

Methods Mol Biol (2007): 3

G protein-coupled receptor internalization assays in the high-content screening format

Authors:

Haasen D, Schnapp A, Valler MJ, Heilker R.

Journal:

Methods Enzymol (2006): 121

Spectral properties

Excitation (nm)	494
Emission (nm)	514

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Fluorescence microscope
Excitation	FITC filter
Emission	FITC filter
Recommended plate	Black wall/clear bottom

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Page updated on July 16, 2026

Component A: Calcein Green™	2 vials
Component B: HHBS (Hanks' buffer with 20 mM Hepes)	1 bottle (100 mL)