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Cell Explorer™ Live Cell Labeling Kit
Green Fluorescence
Our Cell Explorer™ fluorescence imaging kits are a set of tools for labeling cells for fluorescence microscopic investigations of cellular functions. The effective labeling of cells provides a powerful method for studying cellular events in a spatial and temporal context. This particular kit is designed to uniformly label live cells in green fluorescence. The kit uses non-fluorescent Calcein Green™ that becomes strongly fluorescent upon entering into live cells. Calcein Green™ is a hydrophobic compound that easily permeates intact live cells. The hydrolysis of the non-fluorescent Calcein Green™ by intracellular esterases generates the strongly fluorescent hydrophilic Calcein Green™ fluorophore that is well-retained in the cell cytoplasm. Cells grown in black-walled plates can be stained and quantified in less than two hours. It can be readily adapted for high-throughput assays in a wide variety of fluorescence platforms such as microplate assays, immunocytochemistry and flow cytometry. It is useful for a variety of studies, including cell adhesion, chemotaxis, multidrug resistance, cell viability, apoptosis and cytotoxicity. The kit provides all the essential components with an optimized cell-labeling protocol.
Image of HeLa cells stained with Cell Explorer™ Live Cell Labeling Kit *Green Fluorescence* (Cat#22607) in a Costar black wall/clear bottom 96-well plate. Cells were stained with Calcein Green™ for 30 minutes. Images were aquired with fluorescence microscopy with FITC filter.
Image of HeLa cells stained with Cell Explorer™ Live Cell Labeling Kit *Green Fluorescence* (Cat#22607) in a Costar black wall/clear bottom 96-well plate. Cells were stained with Calcein Green™ for 30 minutes. Images were aquired with fluorescence microscopy with FITC filter.
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
22607200 Tests
Price
 
Spectral properties
Excitation (nm)494
Emission (nm)514
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12352200
Instrument settings
Fluorescence microscope
ExcitationFITC filter
EmissionFITC filter
Recommended plateBlack wall/clear bottom
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Page updated on October 8, 2025