Cell Explorer™ Live Cell Labeling Kit is designed for red fluorescent labeling of living cells using Calcein Deep Red™. The cell-permeable, nonfluorescent substrate enters cells, where intracellular esterases hydrolyze it to produce a strongly fluorescent, hydrophilic product. Retention of this product in the cytoplasm enables fluorescence-based detection of labeled cells.

The kit includes Calcein Deep Red™ and HHBS buffer, with sufficient reagents for 200 tests. Fluorescence can be detected by microscopy using a Cy5-compatible filter set, and the labeling approach can be adapted to compatible microplate and flow cytometry platforms. Cell Explorer™ Live Cell Labeling Kit supports live-cell imaging, cell adhesion and chemotaxis studies, and fluorescence-based cell assays.