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Cell Explorer™ Live Cell Labeling Kit
Red Fluorescence
Our Cell Explorer™ fluorescence imaging kits are a set of tools for labeling cells for fluorescence microscopic investigations of cellular functions. The effective labeling of cells provides a powerful method for studying cellular events in a spatial and temporal context. This particular kit is designed to uniformly label live cells in red fluorescence. The kit uses a proprietary non-fluorescent dye that becomes strongly fluorescence upon entering into live cells. The dye is a hydrophobic compound that easily permeates intact live cells. The hydrolysis of the non-fluorescent substrate by intracellular esterases generates a strongly red fluorescent hydrophilic product that is well-retained in the cell cytoplasm. Cells grown in black-walled plates can be stained and quantified in less than two hours. The assay is more robust than the tetrazolium salt or Alarmar Blue™-based assays. It can be readily adapted for high-throughput assays in a wide variety of fluorescence platforms such as microplate assays, immunocytochemistry and flow cytometry. It is useful in a variety of studies, including cell adhesion, chemotaxis, multidrug resistance, cell viability, apoptosis and cytotoxicity. The kit provides all the essential components with an optimized cell-labeling protocol.
Image of HeLa cells stained with Cell Explorer™ Live Cell Labeling Kit *Red Fluorescence* (Cat#22609)in a Costar black wall/clear bottom 96-well plate. Cells were stained with Calcein Deep Red™ for 30 minutes and image was acquired with fluorescence microscope using Cy5 filter.
Image of HeLa cells stained with Cell Explorer™ Live Cell Labeling Kit *Red Fluorescence* (Cat#22609)in a Costar black wall/clear bottom 96-well plate. Cells were stained with Calcein Deep Red™ for 30 minutes and image was acquired with fluorescence microscope using Cy5 filter.
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
22609200 Tests
Price
 
Spectral properties
Excitation (nm)643
Emission (nm)663
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12352200
Instrument settings
Fluorescence microscope
ExcitationCy5 filter set
EmissionCy5 filter set
Recommended plateBlack wall/clear bottom
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Page updated on October 8, 2025