Cell Meter™ Live Cell Caspase 3/7 Binding Assay Kit *Red Fluorescence*

Cell Meter™ Live Cell Caspase 3/7 Binding Assay Kit

Red Fluorescence

Our Cell Meter™ live cell caspases activity assay kits are based on fluorescent FMK inhibitors of caspases. These inhibitors are cell permeable and non-cytotoxic. Once inside the cell, the caspase inhibitors bind covalently to the active caspases. The activation of caspase 3/7 is important for the initiation of apoptosis. It has been proven that caspase 3/7 has substrate selectivity for the peptide sequence Asp-Glu-Val-Asp (DEVD). This kit uses TF3-DEVD-FMK as a fluorescent indicator for caspase 3/7 activity. TF3-DEVD-FMK irreversibly binds to activated caspase 3/7 in apoptotic cells. Once bound to caspase 3/7, the fluorescent reagent is retained inside the cell. The binding event inhibits caspase 3/7 but will not stop apoptosis from proceeding. There are a variety of parameters that can be used for monitoring cell apoptosis. This Cell Meter™ Live Cell Caspase 3/7 Activity Assay Kit is designed to detect cell apoptosis by measuring caspase 3/7 activation in live cells. It is used for the quantification of activated caspase 3/7 activities in apoptotic cells, or for screening caspase 3/7 inhibitors. TF3-DEVD-FMK, the red label reagent, allows for direct detection of activated caspase 3/7 in apoptotic cells by fluorescence microscopy, flow cytometer, or fluorescent microplate reader. The kit provides all the essential components with an optimized assay protocol.

Fluorometric detection of active caspases 3/7 using TF3-DEVD-FMK (Cat# 20101) in Jurkat cells. The cells were treated with 1 μM staurosporine for 4 hours (Red) while untreated cells were used as a control (Green). Control and treated cells were incubated with TF3-DEVD-FMK for 1 hour at 37 °C, and then washed once after stain.  Fluorescent intensity was measured with NovoCyte™ 3000 flow cytometer blue laser excitation/PE emission channel.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
20101	25 Tests	Price

Citations

View all 12 citations: Citation Explorer

Executioner caspases degrade essential mediators of pathogen-host interactions to inhibit growth of intracellular Listeria monocytogenes

Authors:

Lavergne, Marilyne and Schaerer, Raffael and De Grandis, Sara and Bouheraoua, Safaa and Adenuga, Oluwadamilola and Muralt, Tanja and Schaerer, Tiffany and Ch{\`e}vre, L{\'e}a and Failla, Alessandro and Matthey, Patricia and others,

Journal:

Cell Death \& Disease (2025): 55

Isotretinoin promotes elimination of translation-competent HIV latent reservoirs in CD4T cells

Authors:

Howard, J Natalie and Levinger, Callie and Deletsu, Selase and Fromentin, R{\'e}mi and Chomont, Nicolas and Bosque, Alberto and AIDS Clinical Trials Group (ACTG) A5325 Team,

Journal:

PLoS pathogens (2024): e1012601

Executioner caspases degrade essential mediators of pathogen-host interactions to inhibit growth of intracellular Listeria monocytogenes

Authors:

Walch, Michael and Lavergne, Marilyne and Schaerer, Raffael and Bouheraoua, Safaa and Adenuga, Oluwadamilola and Muralt, Tanja and Schaerer, Tiffany and Ch{\`e}vre, L{\'e}a and Failla, Alessandro and Matthey, Patricia and others,

Investigating the Molecular Mechanisms of TMEM16F--a Ca2+ Activated Phospholipid Scramblase and Ion Channel

Authors:

Le, Trieu Phuong Hai

Evidence that polyphenols do not inhibit the phospholipid scramblase TMEM16F

Authors:

Le, Trieu and Le, Son C and Zhang, Yang and Liang, Pengfei and Yang, Huanghe

Journal:

Journal of Biological Chemistry (2020): 12537--12544

References

View all 50 references: Citation Explorer

In vitro effect of different mediators of apoptosis on canine cranial and caudal cruciate ligament fibroblasts and its reversibility by pancaspase inhibitor zVAD.fmk

Authors:

Forterre S, Zurbriggen A, Spreng D.

Journal:

Vet Immunol Immunopathol (2011): 264

Intracochlear perfusion of leupeptin and z-VAD-FMK: influence of antiapoptotic agents on gunshot-induced hearing loss

Authors:

Abaamrane L, Raffin F, Schmerber S, Sendowski I.

Journal:

Eur Arch Otorhinolaryngol (2011): 987

Structure of human caspase-6 in complex with Z-VAD-FMK: New peptide binding mode observed for the non-canonical caspase conformation

Authors:

Muller I, Lamers MB, Ritchie AJ, Dominguez C, Munoz-Sanjuan I, Kiselyov A.

Journal:

Bioorg Med Chem Lett (2011): 5244

Experimental study on treatment of rabbits optic nerve injury with Caspase-3 inhibitor z-DEVD-fmk

Authors:

Zhang W, Yu JG, Wang X, Shen ZS, Zhang JK, Yan H.

Journal:

Zhonghua Yan Ke Za Zhi (2010): 1084

Caspase inhibitor ZVAD-fmk facilitates engraftment of donor hematopoietic stem cells in intra-bone marrow-bone marrow transplantation

Authors:

Imai Y, Adachi Y, Shi M, Shima C, Yanai S, Okigaki M, Yamashima T, Kaneko K, Ikehara S.

Journal:

Stem Cells Dev (2010): 461

Spectral properties

Correction factor (280 nm)	0.179
Extinction coefficient (cm ^-1 M ^-1)	75000 ¹
Excitation (nm)	553
Emission (nm)	578
Quantum yield	0.1 ¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
UNSPSC	12352200

Instrument settings

Flow cytometer
Excitation	550 nm
Emission	595 nm
Instrument specification(s)	FL1 Channel

Fluorescence microscope
Excitation	TRITC channel
Emission	TRITC channel
Recommended plate	Black wall/clear bottom
Instrument specification(s)	FITC channel for Nuclear Green™ DCS1 staining, DAPI channel for Hoechst staining

Fluorescence microplate reader
Excitation	550 nm
Emission	595 nm
Cutoff	570 nm
Recommended plate	Black wall/clear bottom
Instrument specification(s)	Bottom read mode

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Email	sales@aatbio.com
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Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 8, 2025

Component A: TF3-DEVD-FMK	1 vial
Component B: Washing Buffer	1 bottle (100 mL)
Component C: 500X Nuclear Green™ DCS1	1 vial (100 µL)
Component D: 500X Hoechst	1 vial (100 µL)