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Cell Meter™ Live Cell Caspase 3/7 Binding Assay Kit
Red Fluorescence
Our Cell Meter™ live cell caspases activity assay kits are based on fluorescent FMK inhibitors of caspases. These inhibitors are cell permeable and non-cytotoxic. Once inside the cell, the caspase inhibitors bind covalently to the active caspases. The activation of caspase 3/7 is important for the initiation of apoptosis. It has been proven that caspase 3/7 has substrate selectivity for the peptide sequence Asp-Glu-Val-Asp (DEVD). This kit uses TF3-DEVD-FMK as a fluorescent indicator for caspase 3/7 activity. TF3-DEVD-FMK irreversibly binds to activated caspase 3/7 in apoptotic cells. Once bound to caspase 3/7, the fluorescent reagent is retained inside the cell. The binding event inhibits caspase 3/7 but will not stop apoptosis from proceeding. There are a variety of parameters that can be used for monitoring cell apoptosis. This Cell Meter™ Live Cell Caspase 3/7 Activity Assay Kit is designed to detect cell apoptosis by measuring caspase 3/7 activation in live cells. It is used for the quantification of activated caspase 3/7 activities in apoptotic cells, or for screening caspase 3/7 inhibitors. TF3-DEVD-FMK, the red label reagent, allows for direct detection of activated caspase 3/7 in apoptotic cells by fluorescence microscopy, flow cytometer, or fluorescent microplate reader. The kit provides all the essential components with an optimized assay protocol.
Fluorometric detection of active caspases 3/7 using TF3-DEVD-FMK (Cat# 20101) in Jurkat cells. The cells were treated with 1 μM staurosporine for 4 hours (Red) while untreated cells were used as a control (Green). Control and treated cells were incubated with TF3-DEVD-FMK for 1 hour at 37 °C, and then washed once after stain.  Fluorescent intensity was measured with NovoCyte™ 3000 flow cytometer blue laser excitation/PE emission channel.
Fluorometric detection of active caspases 3/7 using TF3-DEVD-FMK (Cat# 20101) in Jurkat cells. The cells were treated with 1 μM staurosporine for 4 hours (Red) while untreated cells were used as a control (Green). Control and treated cells were incubated with TF3-DEVD-FMK for 1 hour at 37 °C, and then washed once after stain.  Fluorescent intensity was measured with NovoCyte™ 3000 flow cytometer blue laser excitation/PE emission channel.
protocol
Protocol
sds
SDS
CatalogSize
Price
Quantity
2010125 Tests
Price
 
Spectral properties
Correction factor (280 nm)0.179
Extinction coefficient (cm -1 M -1)
75000
1
Excitation (nm)553
Emission (nm)578
Quantum yield
0.1
1
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12352200
Instrument settings
Flow cytometer
Excitation550 nm
Emission595 nm
Instrument specification(s)FL1 Channel
Fluorescence microscope
ExcitationTRITC channel
EmissionTRITC channel
Recommended plateBlack wall/clear bottom
Instrument specification(s)FITC channel for Nuclear Green™ DCS1 staining, DAPI channel for Hoechst staining
Fluorescence microplate reader
Excitation550 nm
Emission595 nm
Cutoff570 nm
Recommended plateBlack wall/clear bottom
Instrument specification(s)Bottom read mode
Documents
Protocol
Safety Data Sheet
Certificate of Analysis
Brochure: Cell Apoptosis & Proliferation
Brochure: Enzyme Probes & Assay Kits
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Page updated on October 29, 2025