Cell Navigator® Live Cell Endoplasmic Reticulum (ER) Staining Kit *Red Fluorescence*
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Spectral properties
Excitation (nm) | 588 |
Emission (nm) | 620 |
Storage, safety and handling
Certificate of Origin | Download PDF |
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
UNSPSC | 12352200 |
Alternative formats
Cell Navigator® Live Cell Endoplasmic Reticulum (ER) Staining Kit *Blue Fluorescence* |
Cell Navigator® Live Cell Endoplasmic Reticulum (ER) Staining Kit *Green Fluorescence* |
Related products
Overview | ![]() ![]() |
See also: Endoplasmic Reticulum (ER), Mitochondria
Excitation (nm) 588 | Emission (nm) 620 |
The endoplasmic reticulum (ER) is a type of organelle in the cells of eukaryotic organisms that forms an interconnected network of flattened, membrane-enclosed sacs or tube-like structures known as cisternae. The membranes of the ER are continuous with the outer nuclear membrane. ER occurs in most types of eukaryotic cells, but is absent from red blood cells and spermatozoa. This Cell Navigator® Live Cell Endoplasmic Reticulum (ER) Staining Kit uses our ER Tracer™ Red as an ER marker. ER Tracer™ Red stain is a cell-permeant fluorescent dye that is highly selective for ER. This stain consists of a red fluorescent dye and ER binder that selectively bind to ER in most of cell types. For some cells, ER Tracer™ Red may not selectively bind to ER. ER Tracer™ Red has spectral properties essentially identical to Texas Red, making this kit convenient with the Texas Red filter set.
Platform
Fluorescence microscope
Excitation | 590 nm |
Emission | 620 nm |
Recommended plate | Black wall/clear bottom |
Instrument specification(s) | TRITC or Cy3 filter |
Components
Example protocol
AT A GLANCE
Protocol Summary
- Prepare cells in growth medium
- Incubate cells with ER Tracer™ Red working solution at 37 °C for 15 - 30 minutes
- Analyze under fluorescence microscope at Ex/Em = 590/620 nm (TRITC or Cy3 filter set)
CELL PREPARATION
For guidelines on cell sample preparation, please visit https://www.aatbio.com/resources/guides/cell-sample-preparation.html
PREPARATION OF STOCK SOLUTIONS
Unless otherwise noted, all unused stock solutions should be divided into single-use aliquots and stored at -20 °C after preparation. Avoid repeated freeze-thaw cycles.
Note 20 µL of 500X ER Tracer™ Red stock solution is enough for one 96-well plate. Unused 500X ER Tracer™ Red stock solution can be stored at ≤ -20 ºC for two weeks if the tubes are sealed tightly. Protect from light.
ER Tracer™ Red stock solution (500X)
Add 20 µL of DMSO (Component C) into the vial of ER Tracer™ Red (Component A) and mix well to make 500X ER Tracer™ Red stock solution.Note 20 µL of 500X ER Tracer™ Red stock solution is enough for one 96-well plate. Unused 500X ER Tracer™ Red stock solution can be stored at ≤ -20 ºC for two weeks if the tubes are sealed tightly. Protect from light.
PREPARATION OF WORKING SOLUTION
ER Tracer™ Red working solution
Add 20 µL of 500X ER Tracer™ Red stock solution into 10 mL of Live Cell Staining Buffer (Component B), and mix well to make ER Tracer™ Red working solution.Note This ER Tracer™ Red working solution is stable for at least 2 hours at room temperature. Protect from light.
SAMPLE EXPERIMENTAL PROTOCOL
- Add 100 µL/well (96-well plate) or 50 µL/well (384-well plate) of ER Tracer™ Red working solution in the cell plate. Incubate cells with working solution at 37 °C for 15 - 30 minutes, protected from light.
Note The optimal concentration of the ER probe varies depending on the specific application. Concentration higher than the working solution can be toxic to cells. The staining conditions may be modified according to the particular cell type and the permeability of the cells or tissues to the probe. - Remove ER Tracer™ Red working solution in each well. Wash cells with physically relevant buffer three times.
- Fix cells after staining (Optional). Fix the cells with 4% formaldehyde for 5 - 10 minutes. Wash cells with physically relevant buffer three times.
- Observe the fluorescence signal in cells using fluorescence microscope with TRITC or Cy3 filter set (Ex/Em = 590/620 nm).
Product Family
Name | Excitation (nm) | Emission (nm) |
Cell Navigator® Live Cell Endoplasmic Reticulum (ER) Staining Kit *Blue Fluorescence* | 344 | 457 |
Cell Navigator® Live Cell Endoplasmic Reticulum (ER) Staining Kit *Green Fluorescence* | 503 | 511 |
Images

Figure 1. Fluorescence images of endoplasmic reticulum (ER) staining in HeLa cells cultured in a 96-well black-wall clear-bottom plate using a fluorescence microscope equipped with a TRITC filter set. Live cells were stained with Cell Navigator® Live Cell Endoplasmic Reticulum (ER) Staining Kit *Red Fluorescence* (Cat#22636, Red), mitochondria dye MitoLite™ Green (Cat#22675, Green), and nuclei stain Hoechst 33342 (Cat#17530, Blue).

Figure 2. Fluorescence images of endoplasmic reticulum (ER) staining in HeLa cells cultured in a 96-well black-wall clear-bottom plate using a fluorescence microscope equipped with a TRITC filter set. Live cells were stained with Cell Navigator® Live Cell Endoplasmic Reticulum (ER) Staining Kit *Red Fluorescence* (Cat#22636, Red) then fixed with 4% formaldehyde and labeled with F-actin dye iFluor® 488-Phalloidin (Cat#22661, Green) and nuclei stain DAPI (Cat#17507, Blue).