Digoxigenin-11-dUTP 1 mM solution in water

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Physical properties

Molecular weight	1132.87
Solvent	Water

Storage, safety and handling

Certificate of Origin	Download PDF
H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	41116134

Overview SDS Protocol

CAS

129030-36-0

Molecular weight

1132.87

Digoxigenin-11-dUTP can be enzymatically incorporated into DNA/cDNA as substitute for its natural counterpart dTTP. The resulting digoxigenin-labeled DNA/cDNA probes are subsequently detected using digoxigenin-antibodies conjugated with horseradish peroxidase (HRP), alkaline phosphatase (AP) or a fluorescent dye. The 11-atom linker is attached to the C5 position of uridine to minimize the interference of DIG on the substrate incorporation by PCR polymerase. Typically1:2 Digoxigenin-11-dUTP/dTTP ratio for PCR and Nick Translation.

Calculators

Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of Digoxigenin-11-dUTP *1 mM solution in water* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	88.271 µL	441.357 µL	882.714 µL	4.414 mL	8.827 mL
5 mM	17.654 µL	88.271 µL	176.543 µL	882.714 µL	1.765 mL
10 mM	8.827 µL	44.136 µL	88.271 µL	441.357 µL	882.714 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Images

Figure 1. Chemical structure for Digoxigenin-11-dUTP *1 mM solution in water*

Citations

View all 2 citations: Citation Explorer

LncRNA AL592284. 1 facilitates proliferation and metastasis of cervical cancer cells via miR-30a-5p/Vimentin/EMT axis
Authors: Zhang, Jing and Liu, Hong-li and Liu, Jing-bo and Zhang, Yuan and Liu, Jing and Li, Yan-hua
Journal: Biochemical and Biophysical Research Communications (2021): 95--102

ATF4-mediated transcriptional regulation protects against $\beta$-cell loss during endoplasmic reticulum stress in a mouse model
Authors: Kitakaze, Keisuke and Oyadomari, Miho and Zhang, Jun and Hamada, Yoshimasa and Takenouchi, Yasuhiro and Tsuboi, Kazuhito and Inagaki, Mai and Tachikawa, Masanori and Fujitani, Yoshio and Okamoto, Yasuo and others,
Journal: Molecular Metabolism (2021): 101338

References

View all 9 references: Citation Explorer

Fluorescent DNA hybridization probe preparation using amine modification and reactive dye coupling
Authors: Cox WG, Singer VL.
Journal: Biotechniques (2004): 114

A visual DNA chip for simultaneous detection of hepatitis B virus, hepatitis C virus and human immunodeficiency virus type-1
Authors: Wen JK, Zhang XE, Cheng Z, Liu H, Zhou YF, Zhang ZP, Yang JH, Deng JY.
Journal: Biosens Bioelectron (2004): 685

Aminomodified nucleobases: functionalized nucleoside triphosphates applicable for SELEX
Authors: Schoetzau T, Langner J, Moyroud E, Roehl I, Vonhoff S, Klussmann S.
Journal: Bioconjug Chem (2003): 919

Simple method for preparation of fluor/hapten-labeled dUTP
Authors: Nimmakayalu M, Henegariu O, Ward DC, Bray-Ward P.
Journal: Biotechniques (2000): 518

Topology of yeast RNA polymerase II subunits in transcription elongation complexes studied by photoaffinity cross-linking
Authors: Wooddell CI, Burgess RR.
Journal: Biochemistry (2000): 13405

Quantitative analysis of polymerase chain reaction products using biotinylated dUTP incorporation
Authors: Duplaa C, Couffinhal T, Labat L, Moreau C, Lamaziere JM, Bonnet J.
Journal: Anal Biochem (1993): 229

A non-radioisotopic reverse transcriptase assay using biotin-11-deoxyuridinetriphosphate on primer-immobilized microtiter plates
Authors: Urabe T, Sano K, Tanno M, Mizoguchi J, Otani M, Lee MH, Takasaki T, Kusakabe H, Imagawa DT, Nakai M.
Journal: J Virol Methods (1992): 145

Affinity isolation of active murine erythroleukemia cell chromatin: uniform distribution of ubiquitinated histone H2A between active and inactive fractions
Authors: Dawson BA, Herman T, Haas AL, Lough J.
Journal: J Cell Biochem (1991): 166

Non-radioactive labeling and detection of nucleic acids. IV. Synthesis and properties of digoxigenin-modified 2'-deoxyuridine-5'-triphosphates and a photoactivatable analog of digoxigenin (photodigoxigenin)
Authors: Muhlegger K, Huber E, von der Eltz H, Ruger R, Kessler C.
Journal: Biol Chem Hoppe Seyler (1990): 953