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Digoxigenin-11-dUTP *1 mM solution in water*

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Physical properties
Molecular weight1132.87
SolventWater
Storage, safety and handling
Certificate of OriginDownload PDF
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC41116134

OverviewpdfSDSpdfProtocol


CAS
129030-36-0
Molecular weight
1132.87
Digoxigenin-11-dUTP can be enzymatically incorporated into DNA/cDNA as substitute for its natural counterpart dTTP. The resulting digoxigenin-labeled DNA/cDNA probes are subsequently detected using digoxigenin-antibodies conjugated with horseradish peroxidase (HRP), alkaline phosphatase (AP) or a fluorescent dye. The 11-atom linker is attached to the C5 position of uridine to minimize the interference of DIG on the substrate incorporation by PCR polymerase. Typically1:2 Digoxigenin-11-dUTP/dTTP ratio for PCR and Nick Translation.

Calculators


Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of Digoxigenin-11-dUTP *1 mM solution in water* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM88.271 µL441.357 µL882.714 µL4.414 mL8.827 mL
5 mM17.654 µL88.271 µL176.543 µL882.714 µL1.765 mL
10 mM8.827 µL44.136 µL88.271 µL441.357 µL882.714 µL

Molarity calculator

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Citations


View all 2 citations: Citation Explorer
LncRNA AL592284. 1 facilitates proliferation and metastasis of cervical cancer cells via miR-30a-5p/Vimentin/EMT axis
Authors: Zhang, Jing and Liu, Hong-li and Liu, Jing-bo and Zhang, Yuan and Liu, Jing and Li, Yan-hua
Journal: Biochemical and Biophysical Research Communications (2021): 95--102
ATF4-mediated transcriptional regulation protects against $\beta$-cell loss during endoplasmic reticulum stress in a mouse model
Authors: Kitakaze, Keisuke and Oyadomari, Miho and Zhang, Jun and Hamada, Yoshimasa and Takenouchi, Yasuhiro and Tsuboi, Kazuhito and Inagaki, Mai and Tachikawa, Masanori and Fujitani, Yoshio and Okamoto, Yasuo and others,
Journal: Molecular Metabolism (2021): 101338

References


View all 9 references: Citation Explorer
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