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AAT Bioquest

FastClick™ XFD555 Azide

FastClick™ XFD555 Azide contains both the CAG moiety of FastClick (for assisting click efficiency) and Alexa Fluor® 555 fluorophore (as the fluorescence tag) for developing Alexa Fluor® 555-based fluorescent probes. Alexa Fluor® 555 is a commonly used orange-red fluorophore for labeling proteins, nucleic acids, or other biomolecules. It has moderate photostability and excitation that matches the common 555 nm laser line that is equipped with almost most of the major fluorescence instruments. Its conjugates are widely used for imaging and flow cytometry applications in combination with the widely available Cy3 filter set. It is a water-soluble Cy3 derivative that has pH-insensitive fluorescence from pH 4 to pH 10. Alexa Fluor® is a trademark of ThermoFisher Scientific. FastClick™ reagents have been developed by the scientists of AAT Bioquest for enhancing the yield and reaction speed of copper-catalyzed azide-alkyne cycloaddition (CuAAC) reaction. They contain a copper-chelating ligand that significantly stabilizes the Cu(I) oxidation state and thus accelerates the click reaction. They do not require the use of an external copper-chelator (such as the common THPTA or BTTAA). The high concentration of copper chelators is known to have a detrimental effect on DNA/RNA, thus causing biocompatibility issues. The introduction of a copper-chelating moiety at the reporter molecule allows for a dramatic raise of the effective Cu(I) concentration at the reaction site and thus accelerates the reaction. Under extremely mild conditions the FastClick™ azides and alkynes react much faster in high yield compared to the corresponding conventional CuAAC reactions. Click chemistry was developed by K. Barry Sharpless as a robust and specific method of ligating two molecules together.

Spectrum

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)
FastClick™ Cy3 Azide55556915000010.1510.070.073
FastClick™ Cy5 Azide65167025000010.271, 0.420.020.03
FastClick™ Cy7 Azide7567792500000.30.050.036
FastClick™ XFD350 Azide34344119000-0.250.19
FastClick™ XFD488 Azide499520710000.9210.300.11
FastClick™ XFD647 Azide6506712390000.3310.000.03
FastClick™ XFD750 Azide7527762400000.1210.000.04

References

View all 50 references: Citation Explorer
Involvement of endoplasmic reticulum stress in trigeminal ganglion corneal neuron injury in dry eye disease.
Authors: Zhang, Jinyu and Lin, Hongbin and Li, Fengxian and Wu, Kaili and Yang, Shuangjian and Zhou, Shiyou
Journal: Frontiers in molecular neuroscience (2023): 1083850
Beneficial effect of electroacupuncture on the distribution of foreign substances in the brain of rats developing depression-like behavior.
Authors: He, Jin-Rong and Rubini, Patrizia and Li, Bao-Man and Yin, Hai-Yan and Tang, Yong and Illes, Peter
Journal: IBRO neuroscience reports (2023): 398-406
Homogeneous immunoassay for cyclopiazonic acid based upon mimotopes and upconversion-resonance energy transfer.
Authors: Pradanas-González, Fernando and Peltomaa, Riikka and Lahtinen, Satu and Luque-Uría, Álvaro and Más, Vicente and Barderas, Rodrigo and Maragos, Chris M and Canales, Ángeles and Soukka, Tero and Benito-Peña, Elena and Moreno-Bondi, María C
Journal: Biosensors & bioelectronics (2023): 115339
Fluorescence Intensity and Fluorescence Lifetime Imaging Microscopies (FLIM) of Cell Differentiation in the Small Intestinal Organoids Using Cholera Toxin.
Authors: Okkelman, Irina A and Dmitriev, Ruslan I
Journal: Methods in molecular biology (Clifton, N.J.) (2023): 171-195
Evaluation of Slowfade Diamond as a buffer for STORM microscopy.
Authors: Boukhatem, Hadjer and Durel, Beatrice and Raimbault, Manon and Laurent, Audrey and Olivier, Nicolas
Journal: Biomedical optics express (2023): 550-558
Page updated on October 8, 2024

Ordering information

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Catalog Number72737
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Physical properties

Molecular weight

1159.25

Solvent

DMSO

Spectral properties

Correction Factor (260 nm)

0.08

Correction Factor (280 nm)

0.08

Extinction coefficient (cm -1 M -1)

150000

Excitation (nm)

553

Emission (nm)

568

Quantum yield

0.11

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
Product Image
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Gallery Image 1
The reaction (Green Bar) of FastClick Cy5 Azide with coumarin alkyne occurs under extremely mild conditions (e.g., [Azide] = 0.02 mM, [Alkyne] = 0.02 mM, [CuSO4] = 0.02 mM, [Sodium Ascorbate] = 5 mM, in 100 mM HEPES) under which the common Cy5 azide does not effectively react with the coumarin alkyne substrate.

Alternative formats