AAT Bioquest

FastClick™ Cy3 Azide

Product Image
Product Image
Gallery Image 1
The reaction (Green Bar) of FastClick Cy5 Azide with coumarin alkyne occurs under extremely mild conditions (e.g., [Azide] = 0.02 mM, [Alkyne] = 0.02 mM, [CuSO4] = 0.02 mM, [Sodium Ascorbate] = 5 mM, in 100 mM HEPES) under which the common Cy5 azide does not effectively react with the coumarin alkyne substrate.
Ordering information
Catalog Number
Unit Size
Add to cart
Additional ordering information
InternationalSee distributors
Bulk requestInquire
Custom sizeInquire
ShippingStandard overnight for United States, inquire for international
Request quotation
Physical properties
Molecular weight843.03
Spectral properties
Correction Factor (260 nm)0.07
Correction Factor (280 nm)0.073
Extinction coefficient (cm -1 M -1)1500001
Excitation (nm)555
Emission (nm)569
Quantum yield0.151
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
Alternative formats
FastClick™ Cy3 Alkyne


See also: Click Chemistry
Molecular weight
Correction Factor (260 nm)
Correction Factor (280 nm)
Extinction coefficient (cm -1 M -1)
Excitation (nm)
Emission (nm)
Quantum yield
FastClick™ Cy3 Azide contains both the moiety of FastClick (for assisting click efficiency) and Cy3 fluorophore (as the fluorescence tag) for developing Cy3-based fluorescent probes. FastClick™ reagents have been developed by the scientists of AAT Bioquest for enhancing the yield and reaction speed of copper-catalyzed azide-alkyne cycloaddition (CuAAC) reaction. They contain a copper-chelating ligand that significantly stabilizes the Cu(I) oxidation state and thus accelerates the click reaction. They do not require the use of an external copper-chelator (such as the common THPTA or BTTAA). The high concentration of copper chelators is known to have a detrimental effect on DNA/RNA, thus causing biocompatibility issues. The introduction of a copper-chelating moiety at the reporter molecule allows for a dramatic raise of the effective Cu(I) concentration at the reaction site and thus accelerates the reaction. Under extremely mild conditions the FastClick™ azides and alkynes react much faster in high yield compared to the corresponding conventional CuAAC reactions. Click chemistry was developed by K. Barry Sharpless as a robust and specific method of ligating two molecules together. Two important characteristics make click chemistry attractive for assembling biomolecules. First, click reactions are bio-orthogonal, thus the click chemistry-functionalized biomolecules would not react with the natural biomolecules that lack a clickable functional group. Second, the reactions proceed with ease under mild conditions, such as at room temperature and in aqueous media.


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of FastClick™ Cy3 Azide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM118.62 µL593.099 µL1.186 mL5.931 mL11.862 mL
5 mM23.724 µL118.62 µL237.239 µL1.186 mL2.372 mL
10 mM11.862 µL59.31 µL118.62 µL593.099 µL1.186 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles


Open in Advanced Spectrum Viewer

Spectral properties

Correction Factor (260 nm)0.07
Correction Factor (280 nm)0.073
Extinction coefficient (cm -1 M -1)1500001
Excitation (nm)555
Emission (nm)569
Quantum yield0.151

Product Family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)
FastClick™ Cy5 Azide65167025000010.271, 0.420.020.03
FastClick™ Cy7 Azide7567792500000.30.050.036
FastClick™ XFD350 Azide34344119000-0.250.19
FastClick™ XFD488 Azide499520710000.9210.300.11
FastClick™ XFD555 Azide5535681500000.110.080.08
FastClick™ XFD647 Azide6506712390000.3310.000.03
FastClick™ XFD750 Azide7527762400000.1210.000.04



View all 50 references: Citation Explorer
Histidine-assisted reduction of arylnitrenes upon photo-activation of phenyl azide chromophores in GFP-like fluorescent proteins.
Authors: Grigorenko, Bella L and Khrenova, Maria G and Jones, D Dafydd and Nemukhin, Alexander V
Journal: Organic & biomolecular chemistry (2024): 337-347
Azide-modified corrole phosphorus complexes for endoplasmic reticulum-targeted fluorescence bioimaging and effective cancer photodynamic therapy.
Authors: Cen, Jing-He and Xie, Qi-Hu and Guo, Geng-Hong and Gao, Long-Jiang and Liao, Yu-Hui and Zhong, Xiao-Ping and Liu, Hai-Yang
Journal: European journal of medicinal chemistry (2024): 116102
Combinatorial fluorescent labeling of live anaerobic bacteria via the incorporation of azide-modified sugars into newly synthesized macromolecules.
Authors: Hajjo, Haitham and Bhardwaj, Neerupma and Gefen, Tal and Geva-Zatorsky, Naama
Journal: Nature protocols (2023): 3767-3786
Lipofuscin labeling through biorthogonal strain-promoted azide-alkyne cycloaddition for the detection of senescent cells.
Authors: Lozano-Torres, Beatriz and Blandez, Juan F and García-Fernández, Alba and Sancenón, Félix and Martínez-Máñez, Ramón
Journal: The FEBS journal (2023): 1314-1325
SNAP-tagging live cells via chelation-assisted copper-catalyzed azide-alkyne cycloaddition.
Authors: Stone, Daniel J and Macias-Contreras, Miguel and Crist, Shaun M and Bucag, Christelle F T and Seo, Gwimoon and Zhu, Lei
Journal: Organic & biomolecular chemistry (2023): 7419-7436
A bio-friendly biotin-coupled and azide-functionalized naphthalimide for real-time endogenous hydrogen sulfide analysis in living cells.
Authors: Cha, Yujin and Gopala, Lavanya and Lee, Min Hee
Journal: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy (2023): 122385
Azide Photochemistry in Acrylic Copolymers for Ultraviolet Cross-Linkable Pressure-Sensitive Adhesives: Optimization, Debonding-on-Demand, and Chemical Modification.
Authors: Abu Bakar, Rohani and Li, Yuman and Hewitson, Oliver P and Roth, Peter J and Keddie, Joseph L
Journal: ACS applied materials & interfaces (2022): 30216-30227
Changes in physiological states of Salmonella Typhimurium measured by qPCR with PMA and DyeTox13 Green Azide after pasteurization and UV treatment.
Authors: Li, Liyan and Fu, Jing and Bae, Sungwoo
Journal: Applied microbiology and biotechnology (2022): 2739-2750
Azide-Locked Prodrug Co-Assembly into Nanoparticles with Indocyanine Green for Chemophotothermal Therapy.
Authors: Hou, Meili and Ye, Mengjie and Liu, Lei and Xu, Mingchuan and Liu, Hongmei and Zhang, Hengbo and Li, Yangfeng and Xu, Zhigang and Li, Baosheng
Journal: Molecular pharmaceutics (2022): 3279-3287
Comparative Study of Nitro- and Azide-Functionalized ZnII -Based Coordination Polymers (CPs) as Fluorescent Turn-On Probes for Rapid and Selective Detection of H2 S in Living Cells.
Authors: Saha, Subhajit and Roy, Pritam Kumar and Maity, Kartik and Mandal, Mahitosh and Biradha, Kumar
Journal: Chemistry (Weinheim an der Bergstrasse, Germany) (2022): e202103830