AAT Bioquest

FastClick™ XFD647 Azide

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The reaction (Green Bar) of FastClick Cy5 Azide with coumarin alkyne occurs under extremely mild conditions (e.g., [Azide] = 0.02 mM, [Alkyne] = 0.02 mM, [CuSO4] = 0.02 mM, [Sodium Ascorbate] = 5 mM, in 100 mM HEPES) under which the common Cy5 azide does not effectively react with the coumarin alkyne substrate.
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Physical properties
Molecular weight1185.29
Spectral properties
Correction Factor (260 nm)0.00
Correction Factor (280 nm)0.03
Extinction coefficient (cm -1 M -1)239000
Excitation (nm)650
Emission (nm)671
Quantum yield0.331
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
Alternative formats
FastClick™ XFD647 Alkyne


See also: Click Chemistry
Molecular weight
Correction Factor (260 nm)
Correction Factor (280 nm)
Extinction coefficient (cm -1 M -1)
Excitation (nm)
Emission (nm)
Quantum yield
FastClick™ XFD647 Azide contains both the CAG moiety of FastClick (for assisting click efficiency) and Alexa Fluor® 647 fluorophore (as the fluorescence tag) for developing Alexa Fluor® 647-based fluorescent probes. It readily reacts with an alkyne-containing biomolecule under extremely mild conditions. Alexa Fluor® 647 is a commonly used deep red fluorophore for labeling proteins, nucleic acids, or other biomolecules. It has moderate photostability and excitation that matches the common 647 nm laser line that is equipped with almost all the major new fluorescence instruments. Its conjugates are widely used for imaging and flow cytometry applications in combination with the widely available Cy5 filter set. It is a water-soluble Cy5 derivative that has pH-insensitive fluorescence from pH 4 to pH 10. Alexa Fluor® is a trademark of ThermoFisher Scientific. FastClick™ reagents have been developed by the scientists of AAT Bioquest for enhancing the yield and reaction speed of copper-catalyzed azide-alkyne cycloaddition (CuAAC) reaction. They contain a copper-chelating ligand that significantly stabilizes the Cu(I) oxidation state and thus accelerates the click reaction. They do not require the use of an external copper-chelator (such as the common THPTA or BTTAA). The high concentration of copper chelators is known to have a detrimental effect on DNA/RNA, thus causing biocompatibility issues. The introduction of a copper-chelating moiety at the reporter molecule allows for a dramatic raise of the effective Cu(I) concentration at the reaction site and thus accelerates the reaction. Under extremely mild conditions the FastClick™ azides and alkynes react much faster in high yield compared to the corresponding conventional CuAAC reactions. Click chemistry was developed by K. Barry Sharpless as a robust and specific method of ligating two molecules together. Two important characteristics make click chemistry attractive for assembling biomolecules. First, click reactions are bio-orthogonal, thus the click chemistry-functionalized biomolecules would not react with the natural biomolecules that lack a clickable functional group. Second, the reactions proceed with ease under mild conditions, such as at room temperature and in aqueous media.


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of FastClick™ XFD647 Azide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM84.368 µL421.838 µL843.675 µL4.218 mL8.437 mL
5 mM16.874 µL84.368 µL168.735 µL843.675 µL1.687 mL
10 mM8.437 µL42.184 µL84.368 µL421.838 µL843.675 µL

Molarity calculator

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Spectral properties

Correction Factor (260 nm)0.00
Correction Factor (280 nm)0.03
Extinction coefficient (cm -1 M -1)239000
Excitation (nm)650
Emission (nm)671
Quantum yield0.331

Product Family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)
FastClick™ Cy3 Azide55556915000010.1510.070.073
FastClick™ Cy5 Azide65167025000010.271, 0.420.020.03
FastClick™ Cy7 Azide7567792500000.30.050.036
FastClick™ XFD350 Azide34344119000-0.250.19
FastClick™ XFD488 Azide499520710000.9210.300.11
FastClick™ XFD555 Azide5535681500000.110.080.08
FastClick™ XFD750 Azide7527762400000.1210.000.04



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