Fluorescein-12-dUTP 1 mM in Tris Buffer (pH 7.5) CAS 214154-36-6

Ordering information

Price
Catalog Number
Unit Size
Quantity

Add to cart

Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Shipping	Standard overnight for United States, inquire for international

Physical properties

Molecular weight	1060.62
Solvent	Water

Spectral properties

Absorbance (nm)	487
Correction Factor (260 nm)	0.32
Correction Factor (280 nm)	0.35
Extinction coefficient (cm ^-1 M ^-1)	80000¹
Excitation (nm)	498
Emission (nm)	517
Quantum yield	0.7900¹, 0.95²

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	41116134

Alternative formats

Fluorescein-12-dUTP *1 mM in Tris Buffer (pH 7.5)*

Overview SDS Protocol

CAS

214154-36-6

Molecular weight

1060.62

Absorbance (nm)

487

Correction Factor (260 nm)

0.32

Correction Factor (280 nm)

0.35

Extinction coefficient (cm ^-1 M ^-1)

80000¹

Excitation (nm)

498

Emission (nm)

517

Quantum yield

0.7900¹, 0.95²

The dye-modified deoxyuridine 5'-triphosphates (such as aminoallyl-dUTP) can be used to produce dye-containing DNA by conventional enzymatic incorporation methods such as reverse transcription, nick translation, random primed labeling, or PCR. This enzymatic fluorescence labeling method is widely used for both FISH probes and microarray-based experiments. This fluorescein-dUTP conjugate can be used as a green fluorescence color with Spectrum Green™ filter set (Spectrum Green™ is the trademark of Vysis).

Example protocol

AT A GLANCE

Important notes

Best stored at -80 °C, it can be diluted 10 folds in TE Buffer (10mM Tris-HCl + 1mM EDTA (pH=7.5)) for convenient pipetting. Expiration date is six months from the date of receipt.

SAMPLE EXPERIMENTAL PROTOCOL

Table:1. The following instruction is recommended as a starting point for labeling ~1µg dsDNA, optimum labeling conditions may vary for different cases.

Reagents	Final Concentration for 20µL reaction volume
DNA	0.05 µg/µL
DNA Polymerase	25 ~ 50U/mL
Fluorescein-dUTP	10 ~ 100 µM
Reaction Buffer	10 mM Tris-HCl (pH = 7.5) contains the following components: 1 mM EDTA 5 mM NaCl 0.1 mM DTT 1 mM dATP + 1 mM dCTP+ 1 mM dGTP+ 1 mM dTTP
ddH₂O	Adjust volume as need to make 20 µL reaction volume

Calculators

Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of Fluorescein-12-dUTP *1 mM in Tris Buffer (pH 7.5)* *CAS 214154-36-6* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	94.284 µL	471.422 µL	942.845 µL	4.714 mL	9.428 mL
5 mM	18.857 µL	94.284 µL	188.569 µL	942.845 µL	1.886 mL
10 mM	9.428 µL	47.142 µL	94.284 µL	471.422 µL	942.845 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
	/		=		x		=

Spectrum

Open in Advanced Spectrum Viewer

Spectral properties

Absorbance (nm)	487
Correction Factor (260 nm)	0.32
Correction Factor (280 nm)	0.35
Extinction coefficient (cm ^-1 M ^-1)	80000¹
Excitation (nm)	498
Emission (nm)	517
Quantum yield	0.7900¹, 0.95²

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Quantum yield	Correction Factor (260 nm)	Correction Factor (280 nm)
Fluorescein-12-dCTP 1 mM in Tris Buffer (pH 7.5)	498	517	80000¹	0.7900¹, 0.95²	0.32	0.35
Fluorescein-12-dATP 1 mM in Tris Buffer (pH 7.5)	498	517	80000¹	0.7900¹, 0.95²	0.32	0.35
Fluorescein-12-dGTP 1 mM in Tris Buffer (pH 7.5)	498	517	80000¹	0.7900¹, 0.95²	0.32	0.35

Images

Figure 1. Chemical structure for Fluorescein-12-dUTP *1 mM in Tris Buffer (pH 7.5)* *CAS 214154-36-6*

References

View all 9 references: Citation Explorer

Fluorescent DNA hybridization probe preparation using amine modification and reactive dye coupling
Authors: Cox WG, Singer VL.
Journal: Biotechniques (2004): 114

A visual DNA chip for simultaneous detection of hepatitis B virus, hepatitis C virus and human immunodeficiency virus type-1
Authors: Wen JK, Zhang XE, Cheng Z, Liu H, Zhou YF, Zhang ZP, Yang JH, Deng JY.
Journal: Biosens Bioelectron (2004): 685

Aminomodified nucleobases: functionalized nucleoside triphosphates applicable for SELEX
Authors: Schoetzau T, Langner J, Moyroud E, Roehl I, Vonhoff S, Klussmann S.
Journal: Bioconjug Chem (2003): 919

Simple method for preparation of fluor/hapten-labeled dUTP
Authors: Nimmakayalu M, Henegariu O, Ward DC, Bray-Ward P.
Journal: Biotechniques (2000): 518

Topology of yeast RNA polymerase II subunits in transcription elongation complexes studied by photoaffinity cross-linking
Authors: Wooddell CI, Burgess RR.
Journal: Biochemistry (2000): 13405

Quantitative analysis of polymerase chain reaction products using biotinylated dUTP incorporation
Authors: Duplaa C, Couffinhal T, Labat L, Moreau C, Lamaziere JM, Bonnet J.
Journal: Anal Biochem (1993): 229

A non-radioisotopic reverse transcriptase assay using biotin-11-deoxyuridinetriphosphate on primer-immobilized microtiter plates
Authors: Urabe T, Sano K, Tanno M, Mizoguchi J, Otani M, Lee MH, Takasaki T, Kusakabe H, Imagawa DT, Nakai M.
Journal: J Virol Methods (1992): 145

Affinity isolation of active murine erythroleukemia cell chromatin: uniform distribution of ubiquitinated histone H2A between active and inactive fractions
Authors: Dawson BA, Herman T, Haas AL, Lough J.
Journal: J Cell Biochem (1991): 166

Non-radioactive labeling and detection of nucleic acids. IV. Synthesis and properties of digoxigenin-modified 2'-deoxyuridine-5'-triphosphates and a photoactivatable analog of digoxigenin (photodigoxigenin)
Authors: Muhlegger K, Huber E, von der Eltz H, Ruger R, Kessler C.
Journal: Biol Chem Hoppe Seyler (1990): 953