Fluorescein-12-dUTP (Perkin-Elmer) 1 mM in Tris Buffer (pH 7.5)

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Telephone	1-800-990-8053
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Physical properties

Molecular weight	992.67
Solvent	Water

Spectral properties

Absorbance (nm)	487
Correction Factor (260 nm)	0.32
Correction Factor (280 nm)	0.35
Extinction coefficient (cm ^-1 M ^-1)	80000¹
Excitation (nm)	498
Emission (nm)	517
Quantum yield	0.7900¹, 0.95²

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

Molecular weight

992.67

Absorbance (nm)

487

Correction Factor (260 nm)

0.32

Correction Factor (280 nm)

0.35

Extinction coefficient (cm ^-1 M ^-1)

80000¹

Excitation (nm)

498

Emission (nm)

517

Quantum yield

0.7900¹, 0.95²

The dye-modified deoxyuridine 5'-triphosphates (such as aminoallyl-dUTP) can be used to produce dye-containing DNA by conventional enzymatic incorporation methods such as reverse transcription, nick translation, random primed labeling, or PCR. This enzymatic fluorescence labeling method is widely used for both FISH probes and microarray-based experiments. This fluorescein-12-dUTP conjugate can be used as a green fluorescence color with Spectrum Green™ filter set (Spectrum Green™ is the trademark of Vysis). It is chemically same to fluorescein-12-dUTP (Perkin Elmer’s NEL413001EA).

Calculators

Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of Fluorescein-12-dUTP (Perkin-Elmer) *1 mM in Tris Buffer (pH 7.5)* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	100.738 µL	503.692 µL	1.007 mL	5.037 mL	10.074 mL
5 mM	20.148 µL	100.738 µL	201.477 µL	1.007 mL	2.015 mL
10 mM	10.074 µL	50.369 µL	100.738 µL	503.692 µL	1.007 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
	/		=		x		=

Spectrum

Open in Advanced Spectrum Viewer

Spectral properties

Absorbance (nm)	487
Correction Factor (260 nm)	0.32
Correction Factor (280 nm)	0.35
Extinction coefficient (cm ^-1 M ^-1)	80000¹
Excitation (nm)	498
Emission (nm)	517
Quantum yield	0.7900¹, 0.95²

Images

Figure 1. Chemical structure for Fluorescein-12-dUTP (Perkin-Elmer) *1 mM in Tris Buffer (pH 7.5)* .

References

View all 23 references: Citation Explorer

Anti-proliferative effect of Fe(III) complexed with 1-(2-hydroxy-3-methoxybenzaldehyde)-4-aminosalicylhydrazone in HepG2 cells.
Authors: Fukushima, Takeshi and Taniguchi, Erina and Yamada, Hiroshi and Kato, Kiyomasa and Shimizu, Ayako and Nishiguchi, Yoshikazu and Onozato, Mayu and Ichiba, Hideaki and Azuma, Yutaro
Journal: Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine (2015): 669-77

Localisation of abundant and organ-specific genes expressed in Rosa hybrida leaves and flower buds by direct in situ RT-PCR.
Authors: Jedrzejuk, Agata and Mibus, Heiko and Serek, Margrethe
Journal: TheScientificWorldJournal (2012): 609597

SNP genotyping using microsphere-linked PNA and flow cytometric detection.
Authors: Rockenbauer, Eszter and Petersen, Kenneth and Vogel, Ulla and Bolund, Lars and Kølvraa, Steen and Nielsen, Kirsten Vang and Nexø, Bjørn A
Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2005): 80-6

[Comparative genomic hybridization analysis of nasopharyngeal carcinoma drug-resistant cell CNE2/DDP and its parent cell CNE2].
Authors: Jiang, Run-De and Hu, Liang and Guan, Xin-Yuan and Zhang, Li-Xin and Yue, Wen and Cen, Xin-Tang and Li, Chun-Hai
Journal: Ai zheng = Aizheng = Chinese journal of cancer (2004): 386-90

Flow cytometric detection of beta-D-glucuronidase gene in wild-type bacterial cells using in-situ PCR.
Authors: Sachidanandham, Ramaiah and Gin, Karina Yew-Hoong
Journal: Biotechnology and bioengineering (2003): 127-33

Lack of nuclear apoptosis in cardiomyocytes and increased endothelin-1 levels in a rat heart model of myocardial stunning.
Authors: Klainguti, M and Aigner, S and Kilo, J and Eppenberger, H M and Mandinova, A and Aebi, U and Schaub, M C and Shaw, S G and Lüscher, T F and Atar, D
Journal: Basic research in cardiology (2000): 308-15

A method to monitor DNA transfer during transfection.
Authors: Johnson, A L and Jurcisek, J A and Trask, O J and Au, J L
Journal: AAPS pharmSci (1999): E6

Pulsatile shear stress leads to DNA fragmentation in human SH-SY5Y neuroblastoma cell line.
Authors: Triyoso, D H and Good, T A
Journal: The Journal of physiology (1999): 355-65

Detection of TNF alpha and Fas ligand mRNA within synovial mononuclear cells by fluorescence in-cell labeling PCR (FICL-PCR).
Authors: Okubo, M and Brown, M P and Chiba, K and Kasukawa, R and Nishimaki, T
Journal: Molecular biology reports (1998): 217-24

Targeted nucleotide exchange in the alkaline phosphatase gene of HuH-7 cells mediated by a chimeric RNA/DNA oligonucleotide.
Authors: Kren, B T and Cole-Strauss, A and Kmiec, E B and Steer, C J
Journal: Hepatology (Baltimore, Md.) (1997): 1462-8