iFluor® 440-dUTP 1 mM in Tris Buffer (pH 7.5)

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Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Shipping	Standard overnight for United States, inquire for international

Physical properties

Molecular weight	994.73
Solvent	Water

Spectral properties

Absorbance (nm)	430
Correction Factor (260 nm)	0.352
Correction Factor (280 nm)	0.229
Extinction coefficient (cm ^-1 M ^-1)	40000¹
Excitation (nm)	434
Emission (nm)	480
Quantum yield	0.67¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

Molecular weight

994.73

Absorbance (nm)

430

Correction Factor (260 nm)

0.352

Correction Factor (280 nm)

0.229

Extinction coefficient (cm ^-1 M ^-1)

40000¹

Excitation (nm)

434

Emission (nm)

480

Quantum yield

0.67¹

The dye-modified deoxyuridine 5'-triphosphates (such as aminoallyl-dUTP) can be used to produce dye-containing DNA by conventional enzymatic incorporation methods such as reverse transcription, nick translation, random primed labeling, or PCR. This enzymatic fluorescence labeling method is widely used for both FISH probes and microarray-based experiments. DEAC-dUTP conjugate (#17011) is widely used as a blue fluorescence color with SpectrumAqua™ filter set (SpectrumAqua™ is the trademark of Vysis) since DEAC and SpectrumAqua have remarkably similar spectral properties. However, the extremely high hydrophobicity of DECA fluorophore make the DEAC conjugates easily adsorbed on plastic surfaces, resulting in high assay errors. iFluor® 440 dyes have excellent water solubility and are designed to be a superior replacement for DEAC dye. iFluor® 440-dUTP conjugate is a superior replacement for DEAC-dUTP conjugate. iFluor® 440 dye conjugates are significantly brighter than the corresponding bioconjugates of DEAC or other spectrally similar dyes (such as SpectrumAqua). iFluor® 440 fluorophore has its spectra well matched with the filter set of SpectrumAqua (SpectrumAqua™ is the trademark of Vysis).

Calculators

Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of iFluor® 440-dUTP *1 mM in Tris Buffer (pH 7.5)* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	100.53 µL	502.649 µL	1.005 mL	5.026 mL	10.053 mL
5 mM	20.106 µL	100.53 µL	201.06 µL	1.005 mL	2.011 mL
10 mM	10.053 µL	50.265 µL	100.53 µL	502.649 µL	1.005 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
	/		=		x		=

Spectrum

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Spectral properties

Absorbance (nm)	430
Correction Factor (260 nm)	0.352
Correction Factor (280 nm)	0.229
Extinction coefficient (cm ^-1 M ^-1)	40000¹
Excitation (nm)	434
Emission (nm)	480
Quantum yield	0.67¹

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Quantum yield	Correction Factor (260 nm)	Correction Factor (280 nm)
iFluor®488-dUTP 1 mM in Tris Buffer (pH 7.5)	491	516	75000¹	0.9¹	0.21	0.11
iFluor® 440 Styramide	434	480	40000¹	0.67¹	0.352	0.229
iFluor® 560-dUTP 1 mM in Tris Buffer (pH 7.5)	560	571	120000¹	0.57¹	0.0482	0.069

Images

Figure 1. Chemical structure for iFluor® 440-dUTP *1 mM in Tris Buffer (pH 7.5)*.

References

View all 8 references: Citation Explorer

MECOM (EVI1) Rearrangements: A Review and Case Report of Two MDS Patients with Complex 3q Inversion/Deletions.
Authors: Lawce, Helen and Szabo, Elina and Torimaru, Yumi and Davis, Craig and Osterberg, Karin and Olson, Susan and Moore, Steve
Journal: Journal of the Association of Genetic Technologists (2017): 9-14

Localization of a female-specific marker on the chromosomes of the brown seaweed Saccharina japonica using fluorescence in situ hybridization.
Authors: Liu, Yu and Bi, YanHui and Gu, JunGang and Li, LiHua and Zhou, ZhiGang
Journal: PloS one (2012): e48784

Application of tri-colour, dual fusion fluorescence in situ hybridization (FISH) system for the characterization of BCR-ABL1 fusion in chronic myelogenous leukaemia (CML) and residual disease monitoring.
Authors: Siu, Lisa Lp and Ma, Edmond Sk and Wong, Wai Shan and Chan, Man Hong and Wong, Kit Fai
Journal: BMC blood disorders (2009): 4

Review of imaging solutions for integrated quantitative immunohistochemistry in the Pathology daily practice.
Authors: Rojo, Marcial García and Bueno, Gloria and Slodkowska, Janina
Journal: Folia histochemica et cytobiologica (2009): 349-54

Comparison of quantitative immunofluorescence with conventional methods for HER2/neu testing with respect to response to trastuzumab therapy in metastatic breast cancer.
Authors: Giltnane, Jennifer M and Molinaro, Annette and Cheng, Huan and Robinson, Andrew and Turbin, Dmitry and Gelmon, Karen and Huntsman, David and Rimm, David L
Journal: Archives of pathology & laboratory medicine (2008): 1635-47

Bladder cancer detection using FISH (UroVysion assay).
Authors: Halling, Kevin C and Kipp, Benjamin R
Journal: Advances in anatomic pathology (2008): 279-86

AQUA and FISH analysis of HER-2/neu expression and amplification in a small cell lung carcinoma tissue microarray.
Authors: Giltnane, J M and Murren, J R and Rimm, D L and King, B L
Journal: Histopathology (2006): 161-9

A novel tricolor, dual-fusion fluorescence in situ hybridization method to detect BCR/ABL fusion in cells with t(9;22)(q34;q11.2) associated with deletion of DNA on the derivative chromosome 9 in chronic myelocytic leukemia.
Authors: Smoley, Stephanie A and Brockman, Stephanie R and Paternoster, Sarah F and Meyer, Reid G and Dewald, Gordon W
Journal: Cancer genetics and cytogenetics (2004): 1-6