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iFluor® 440-dUTP *1 mM in Tris Buffer (pH 7.5)*

Chemical structure for iFluor® 440-dUTP *1 mM in Tris Buffer (pH 7.5)*.
Chemical structure for iFluor® 440-dUTP *1 mM in Tris Buffer (pH 7.5)*.
Ordering information
Price ()
Catalog Number17029
Unit Size
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Additional ordering information
Telephone1-408-733-1055
Fax1-408-733-1304
Emailsales@aatbio.com
InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Physical properties
Molecular weight994.73
SolventWater
Spectral properties
Absorbance (nm)430
Correction Factor (260 nm)0.352
Correction Factor (280 nm)0.229
Extinction coefficient (cm -1 M -1)400001
Excitation (nm)434
Emission (nm)480
Quantum yield0.671
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
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Show More (263)

OverviewpdfSDSpdfProtocol


Molecular weight
994.73
Absorbance (nm)
430
Correction Factor (260 nm)
0.352
Correction Factor (280 nm)
0.229
Extinction coefficient (cm -1 M -1)
400001
Excitation (nm)
434
Emission (nm)
480
Quantum yield
0.671
The dye-modified deoxyuridine 5'-triphosphates (such as aminoallyl-dUTP) can be used to produce dye-containing DNA by conventional enzymatic incorporation methods such as reverse transcription, nick translation, random primed labeling, or PCR. This enzymatic fluorescence labeling method is widely used for both FISH probes and microarray-based experiments. DEAC-dUTP conjugate (#17011) is widely used as a blue fluorescence color with SpectrumAqua™ filter set (SpectrumAqua™ is the trademark of Vysis) since DEAC and SpectrumAqua have remarkably similar spectral properties. However, the extremely high hydrophobicity of DECA fluorophore make the DEAC conjugates easily adsorbed on plastic surfaces, resulting in high assay errors. iFluor® 440 dyes have excellent water solubility and are designed to be a superior replacement for DEAC dye. iFluor® 440-dUTP conjugate is a superior replacement for DEAC-dUTP conjugate. iFluor® 440 dye conjugates are significantly brighter than the corresponding bioconjugates of DEAC or other spectrally similar dyes (such as SpectrumAqua). iFluor® 440 fluorophore has its spectra well matched with the filter set of SpectrumAqua (SpectrumAqua™ is the trademark of Vysis).

Calculators


Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of iFluor® 440-dUTP *1 mM in Tris Buffer (pH 7.5)* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM100.53 µL502.649 µL1.005 mL5.026 mL10.053 mL
5 mM20.106 µL100.53 µL201.06 µL1.005 mL2.011 mL
10 mM10.053 µL50.265 µL100.53 µL502.649 µL1.005 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
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Spectrum


Open in Advanced Spectrum Viewer
spectrum

Spectral properties

Absorbance (nm)430
Correction Factor (260 nm)0.352
Correction Factor (280 nm)0.229
Extinction coefficient (cm -1 M -1)400001
Excitation (nm)434
Emission (nm)480
Quantum yield0.671

Product family


NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)
iFluor®488-dUTP *1 mM in Tris Buffer (pH 7.5)*4915167500010.910.210.11
iFluor® 440 Styramide4344804000010.6710.3520.229

References


View all 8 references: Citation Explorer
MECOM (EVI1) Rearrangements: A Review and Case Report of Two MDS Patients with Complex 3q Inversion/Deletions.
Authors: Lawce, Helen and Szabo, Elina and Torimaru, Yumi and Davis, Craig and Osterberg, Karin and Olson, Susan and Moore, Steve
Journal: Journal of the Association of Genetic Technologists (2017): 9-14
Localization of a female-specific marker on the chromosomes of the brown seaweed Saccharina japonica using fluorescence in situ hybridization.
Authors: Liu, Yu and Bi, YanHui and Gu, JunGang and Li, LiHua and Zhou, ZhiGang
Journal: PloS one (2012): e48784
Application of tri-colour, dual fusion fluorescence in situ hybridization (FISH) system for the characterization of BCR-ABL1 fusion in chronic myelogenous leukaemia (CML) and residual disease monitoring.
Authors: Siu, Lisa Lp and Ma, Edmond Sk and Wong, Wai Shan and Chan, Man Hong and Wong, Kit Fai
Journal: BMC blood disorders (2009): 4
Review of imaging solutions for integrated quantitative immunohistochemistry in the Pathology daily practice.
Authors: Rojo, Marcial García and Bueno, Gloria and Slodkowska, Janina
Journal: Folia histochemica et cytobiologica (2009): 349-54
Comparison of quantitative immunofluorescence with conventional methods for HER2/neu testing with respect to response to trastuzumab therapy in metastatic breast cancer.
Authors: Giltnane, Jennifer M and Molinaro, Annette and Cheng, Huan and Robinson, Andrew and Turbin, Dmitry and Gelmon, Karen and Huntsman, David and Rimm, David L
Journal: Archives of pathology & laboratory medicine (2008): 1635-47
Bladder cancer detection using FISH (UroVysion assay).
Authors: Halling, Kevin C and Kipp, Benjamin R
Journal: Advances in anatomic pathology (2008): 279-86
AQUA and FISH analysis of HER-2/neu expression and amplification in a small cell lung carcinoma tissue microarray.
Authors: Giltnane, J M and Murren, J R and Rimm, D L and King, B L
Journal: Histopathology (2006): 161-9
A novel tricolor, dual-fusion fluorescence in situ hybridization method to detect BCR/ABL fusion in cells with t(9;22)(q34;q11.2) associated with deletion of DNA on the derivative chromosome 9 in chronic myelocytic leukemia.
Authors: Smoley, Stephanie A and Brockman, Stephanie R and Paternoster, Sarah F and Meyer, Reid G and Dewald, Gordon W
Journal: Cancer genetics and cytogenetics (2004): 1-6