iFluor™ 560-dUTP *1 mM in Tris Buffer (pH 7.5)*
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
| Quantity |
Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| Quotation | Request |
| International | See distributors |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Molecular weight | 1115.60 |
| Solvent | Water |
Spectral properties
| Correction Factor (260 nm) | 0.0482 |
| Correction Factor (280 nm) | 0.069 |
| Extinction coefficient (cm -1 M -1) | 1200001 |
| Excitation (nm) | 560 |
| Emission (nm) | 571 |
| Quantum yield | 0.571 |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
| Overview |  SDSProtocol |
Molecular weight 1115.60 | Correction Factor (260 nm) 0.0482 | Correction Factor (280 nm) 0.069 | Extinction coefficient (cm -1 M -1) 1200001 | Excitation (nm) 560 | Emission (nm) 571 | Quantum yield 0.571 |
iFluor™ 560-dUTP is a iFluor 560-modified nucleotide that can be used in various molecular biology applications, such as labeling and detection of DNA molecules. It contains iFluor 560 dye attached to a deoxyuridine triphosphate (dUTP) molecule via a linker arm. When incorporated into DNA during synthesis, the iFluor™ 560-dUTP can be visualized using fluorescence microscopy or other fluorescence-based assays. It is commonly used in techniques such as fluorescence in situ hybridization (FISH), microarray analysis, and real-time PCR. iFluor™ 560-dUTP is preferred over other similar fluorescent dyes due to its high fluorescence intensity, photostability, and compatibility with a wide range of detection systems.
Calculators
Common stock solution preparation
Table 1. Volume of Water needed to reconstitute specific mass of iFluor™ 560-dUTP *1 mM in Tris Buffer (pH 7.5)* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 89.638 µL | 448.189 µL | 896.379 µL | 4.482 mL | 8.964 mL |
| 5 mM | 17.928 µL | 89.638 µL | 179.276 µL | 896.379 µL | 1.793 mL |
| 10 mM | 8.964 µL | 44.819 µL | 89.638 µL | 448.189 µL | 896.379 µL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
| / | = | x | = |
Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
| Correction Factor (260 nm) | 0.0482 |
| Correction Factor (280 nm) | 0.069 |
| Extinction coefficient (cm -1 M -1) | 1200001 |
| Excitation (nm) | 560 |
| Emission (nm) | 571 |
| Quantum yield | 0.571 |
Product Family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
| iFluor® 440-dUTP *1 mM in Tris Buffer (pH 7.5)* | 434 | 480 | 400001 | 0.671 | 0.352 | 0.229 |
| iFluor®488-dUTP *1 mM in Tris Buffer (pH 7.5)* | 491 | 516 | 750001 | 0.91 | 0.21 | 0.11 |
Images
Figure 1. iFluor™ 560-dUTP is a iFluor-modified nucleotide that can be used in various molecular biology applications, such as labeling and detection of DNA molecules. It contains iFluor 560 dye attached to a deoxyuridine triphosphate (dUTP) molecule via a linker arm.