iFluor®555-PEG12-dUTP 1 mM in Tris Buffer (pH 7.5)

Fluorescence In Situ Hybridization of Cy3 and iFluor® 555-dUTP labelled Telomere probes in metaphase HeLa cells.

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Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
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Physical properties

Molecular weight	2454.14
Solvent	Water

Spectral properties

Correction Factor (260 nm)	0.23
Correction Factor (280 nm)	0.14
Extinction coefficient (cm ^-1 M ^-1)	100000¹
Excitation (nm)	557
Emission (nm)	570
Quantum yield	0.64¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

Molecular weight

2454.14

Correction Factor (260 nm)

0.23

Correction Factor (280 nm)

0.14

Extinction coefficient (cm ^-1 M ^-1)

100000¹

Excitation (nm)

557

Emission (nm)

570

Quantum yield

0.64¹

The dye-modified deoxyuridine 5'-triphosphates are one of the most common methods to produce dye-labelled DNA via the conventional enzymatic incorporation methods such as reverse transcription, nick translation, random primed labeling, or PCR. This enzymatic fluorescence labeling method is widely used for both FISH probes and microarray-based experiments. This iFluor®555-PEG12-dUTP conjugate has the same spectral properties to ChromaTide™Alexa Fluor 555-dUTP. Its PEG12 spacer makes it much more effectively incorporated into DNAs by polymerase. It is used as a red fluorescence color with SpectrumOrange™ filter set. It provides a bright signal that has high photostability and is not affected by pH. ChromaTide™ and Alexa Fluor™ are the trademarks of ThermoFisher, and SpectrumOrange™ is the trademark of Vysis.

Calculators

Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of iFluor®555-PEG12-dUTP *1 mM in Tris Buffer (pH 7.5)* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	40.747 µL	203.737 µL	407.475 µL	2.037 mL	4.075 mL
5 mM	8.149 µL	40.747 µL	81.495 µL	407.475 µL	814.949 µL
10 mM	4.075 µL	20.374 µL	40.747 µL	203.737 µL	407.475 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
	/		=		x		=

Spectrum

Open in Advanced Spectrum Viewer

Spectral properties

Correction Factor (260 nm)	0.23
Correction Factor (280 nm)	0.14
Extinction coefficient (cm ^-1 M ^-1)	100000¹
Excitation (nm)	557
Emission (nm)	570
Quantum yield	0.64¹

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Quantum yield	Correction Factor (260 nm)	Correction Factor (280 nm)	Correction Factor (656 nm)
iFluor®488-PEG12-dUTP 1 mM in Tris Buffer (pH 7.5)	491	516	75000¹	0.9¹	0.21	0.11	-
iFluor®647-PEG12-dUTP 1 mM in Tris Buffer (pH 7.5)	656	670	250000¹	0.25¹	0.03	0.03	0.0793

Images

Figure 1. Fluorescence In Situ Hybridization of Cy3 and iFluor® 555-dUTP labelled Telomere probes in metaphase HeLa cells.

Figure 2. Chemical structure for iFluor®555-PEG12-dUTP *1 mM in Tris Buffer (pH 7.5)*.

References

View all 48 references: Citation Explorer

A Fluorescent DNA Hydrogel Aptasensor Based on the Self-Assembly of Rolling Circle Amplification Products for Sensitive Detection of Ochratoxin A.
Authors: Hao, Liling and Wang, Wei and Shen, Xueqing and Wang, Shuliu and Li, Qian and An, Faliang and Wu, Shijia
Journal: Journal of agricultural and food chemistry (2020): 369-375

Engineering nicking enzymes that preferentially nick 5-methylcytosine-modified DNA.
Authors: Gutjahr, Alice and Xu, Shuang-yong
Journal: Nucleic acids research (2014): e77

Digital detection of multiple minority mutants in stool DNA for noninvasive colorectal cancer diagnosis.
Authors: Deng, Lili and Qi, Zongtai and Zou, Binjie and Wu, Haiping and Huang, Huan and Kajiyama, Tomoharu and Kambara, Hideki and Zhou, Guohua
Journal: Analytical chemistry (2012): 5645-52

Digital analysis of the expression levels of multiple colorectal cancer-related genes by multiplexed digital-PCR coupled with hydrogel bead-array.
Authors: Qi, Zongtai and Ma, Yinjiao and Deng, Lili and Wu, Haiping and Zhou, Guohua and Kajiyama, Tomoharu and Kambara, Hideki
Journal: The Analyst (2011): 2252-9

Microisolation and microcloning of bovine X-chromosomes for identification of sorted buffalo (Bubalus bubalis) spermatozoa.
Authors: Zhuang, Xin-jie and Lu, Yang-qing and Zhang, Ming and Lu, Sheng-sheng and Lu, Ke-huan
Journal: Animal reproduction science (2011): 32-6

Selection of reliable reference genes for gene expression study in nasopharyngeal carcinoma.
Authors: Guo, Yi and Chen, Jia-xin and Yang, Shu and Fu, Xu-ping and Zhang, Zheng and Chen, Ke-he and Huang, Yan and Li, Yao and Xie, Yi and Mao, Yu-min
Journal: Acta pharmacologica Sinica (2010): 1487-94

[Expression of cell cycle molecules in human azoospermic testes].
Authors: Yang, Bo and Yuan, Jian-lin and Gao, Xiao-kang and Qin, Wei-jun and Liu, Fei and Shao, Chen and Liu, He-liang and Kang, Fu-xia
Journal: Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology (2009): 393-5

A technical note on quantum dots for multi-color fluorescence in situ hybridization.
Authors: Müller, S and Cremer, M and Neusser, M and Grasser, F and Cremer, T
Journal: Cytogenetic and genome research (2009): 351-9

[Expression of COX10 in human non-obstructive azoospermia testes].
Authors: Yang, Bo and Yuan, Jian-Lin and Gao, Xiao-Kang and Wang, He and Shao, Chen and Liu, He-Liang and Chen, Bao-Qi and Qin, Rong-Liang and Shao, Guo-Xing and Kang, Fu-Xia
Journal: Zhonghua nan ke xue = National journal of andrology (2009): 599-603

[Effect of bicyclol on gene expression profiles in mice with liver injury induced by concanavalin A].
Authors: Yu, Ying-Nan and Guo, Jiang and Li, Ye and Hong, Yuan and Li, Kang and Cheng, Jun and Li, Yan
Journal: Yao xue xue bao = Acta pharmaceutica Sinica (2008): 596-600