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iFluor®647-PEG12-dUTP *1 mM in Tris Buffer (pH 7.5)*

Fluorescence In Situ Hybridization of Cy5 and iFluor® 647-dUTP labelled Telomere probes in metaphase HeLa cells.
Fluorescence In Situ Hybridization of Cy5 and iFluor® 647-dUTP labelled Telomere probes in metaphase HeLa cells.
Chemical structure for iFluor®647-PEG12-dUTP *1 mM in Tris Buffer (pH 7.5)*.
Ordering information
Price ()
Catalog Number17043
Unit Size
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Additional ordering information
Telephone1-408-733-1055
Fax1-408-733-1304
Emailsales@aatbio.com
InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Physical properties
Molecular weight2293.72
SolventWater
Spectral properties
Correction Factor (260 nm)0.03
Correction Factor (280 nm)0.03
Correction Factor (656 nm)0.0793
Extinction coefficient (cm -1 M -1)2500001
Excitation (nm)656
Emission (nm)670
Quantum yield0.251
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
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OverviewpdfSDSpdfProtocol


Molecular weight
2293.72
Correction Factor (260 nm)
0.03
Correction Factor (280 nm)
0.03
Correction Factor (656 nm)
0.0793
Extinction coefficient (cm -1 M -1)
2500001
Excitation (nm)
656
Emission (nm)
670
Quantum yield
0.251
The dye-modified deoxyuridine 5'-triphosphates are one of the most common methods to produce dye-labelled DNA via the conventional enzymatic incorporation methods such as reverse transcription, nick translation, random primed labeling, or PCR. This enzymatic fluorescence labeling method is widely used for both FISH probes and microarray-based experiments. This iFluor®647-PEG12-dUTP conjugate has the same spectral properties to ChromaTide™Alexa Fluor 647-dUTP. Its PEG12 spacer makes it much more effectively incorporated into DNAs by polymerase. It is used as a far red fluorescence color with SpectrumFRed™ filter set. It provides a bright signal that has high photostability and is not affected by pH. ChromaTide™ and Alexa Fluor™ are the trademarks of ThermoFisher, and SpectrumFRed™ is the trademark of Vysis.

Calculators


Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of iFluor®647-PEG12-dUTP *1 mM in Tris Buffer (pH 7.5)* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM43.597 µL217.987 µL435.973 µL2.18 mL4.36 mL
5 mM8.719 µL43.597 µL87.195 µL435.973 µL871.946 µL
10 mM4.36 µL21.799 µL43.597 µL217.987 µL435.973 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
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Spectrum


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spectrum

Spectral properties

Correction Factor (260 nm)0.03
Correction Factor (280 nm)0.03
Correction Factor (656 nm)0.0793
Extinction coefficient (cm -1 M -1)2500001
Excitation (nm)656
Emission (nm)670
Quantum yield0.251

Product family


NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)
iFluor®488-PEG12-dUTP *1 mM in Tris Buffer (pH 7.5)*4915167500010.910.210.11
iFluor®555-PEG12-dUTP *1 mM in Tris Buffer (pH 7.5)*55757010000010.6410.230.14

References


View all 38 references: Citation Explorer
Loss of PDPK1 abrogates resistance to gemcitabine in label-retaining pancreatic cancer cells.
Authors: Li, Dandan and Mullinax, John E and Aiken, Taylor and Xin, Hongwu and Wiegand, Gordon and Anderson, Andrew and Thorgeirsson, Snorri and Avital, Itzhak and Rudloff, Udo
Journal: BMC cancer (2018): 772
dUTPs conjugated with zwitterionic Cy3 or Cy5 fluorophore analogues are effective substrates for DNA amplification and labelling by Taq polymerase.
Authors: Zasedateleva, Olga A and Vasiliskov, Vadim A and Surzhikov, Sergey A and Kuznetsova, Viktoriya E and Shershov, Valeriy E and Guseinov, Timur O and Smirnov, Igor P and Yurasov, Roman A and Spitsyn, Maksim A and Chudinov, Alexander V
Journal: Nucleic acids research (2018): e73
Mass-spectrometry analysis of modifications at DNA termini induced by DNA polymerases.
Authors: Smirnov, Igor P and Kolganova, Natalia A and Vasiliskov, Vadim A and Chudinov, Alexander V and Timofeev, Edward N
Journal: Scientific reports (2017): 6674
Separation of replication and transcription domains in nucleoli.
Authors: Smirnov, E and Borkovec, J and Kováčik, L and Svidenská, S and Schröfel, A and Skalníková, M and Švindrych, Z and Křížek, P and Ovesný, M and Hagen, G M and Juda, P and Michalová, K and Cardoso, M C and Cmarko, D and Raška, I
Journal: Journal of structural biology (2014): 259-66
A prenatally ascertained, maternally inherited 14.8 Mb duplication of chromosomal bands Xq13.2-q21.31 associated with multiple congenital abnormalities in a male fetus.
Authors: Sismani, C and Donoghue, J and Alexandrou, A and Karkaletsi, M and Christopoulou, S and Konstantinidou, A E and Livanos, P and Patsalis, P C and Velissariou, V
Journal: Gene (2013): 138-42
[Establishment of a hydrogel chip for high-throughput detection of Y chromosome microdeletions].
Authors: Li, You-Zhi and Chen, Zhi-Yao and Wang, Hui and Huang, Huan and Song, Qin-Xin and Zhou, Guo-Hua
Journal: Zhonghua nan ke xue = National journal of andrology (2012): 109-14
Selection of reliable reference genes for gene expression study in nasopharyngeal carcinoma.
Authors: Guo, Yi and Chen, Jia-xin and Yang, Shu and Fu, Xu-ping and Zhang, Zheng and Chen, Ke-he and Huang, Yan and Li, Yao and Xie, Yi and Mao, Yu-min
Journal: Acta pharmacologica Sinica (2010): 1487-94
Time-resolved three-dimensional molecular tracking in live cells.
Authors: Wells, Nathan P and Lessard, Guillaume A and Goodwin, Peter M and Phipps, Mary E and Cutler, Patrick J and Lidke, Diane S and Wilson, Bridget S and Werner, James H
Journal: Nano letters (2010): 4732-7
[Expression of cell cycle molecules in human azoospermic testes].
Authors: Yang, Bo and Yuan, Jian-lin and Gao, Xiao-kang and Qin, Wei-jun and Liu, Fei and Shao, Chen and Liu, He-liang and Kang, Fu-xia
Journal: Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology (2009): 393-5
[Expression of COX10 in human non-obstructive azoospermia testes].
Authors: Yang, Bo and Yuan, Jian-Lin and Gao, Xiao-Kang and Wang, He and Shao, Chen and Liu, He-Liang and Chen, Bao-Qi and Qin, Rong-Liang and Shao, Guo-Xing and Kang, Fu-Xia
Journal: Zhonghua nan ke xue = National journal of andrology (2009): 599-603