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Live or Dead™ Cell Viability Assay Kit
Red/Blue Dual Fluorescence
This Live or Dead™ Cell Viability Assay Kit uses two fluorescent indicators: Cellbrite™ Red (Ex/Em = 613/631 nm) for labeling viable cells and a cell-impermeable DNA-binding dye Nuclear Blue™ DCS1 (Ex/Em = 360/450 nm) for labeling dead cells with damaged membranes. Cells grown in black-wall plates can be stained and quantified in less than two hours. The assay is more robust and accurate than the other viability assays. It can be readily adapted for a wide variety of fluorescence platforms such as microplate assays, fluorescence microscopes, and flow cytometry. The kit provides all the essential components with an optimized assay protocol. It is suitable for both proliferating and non-proliferating cells (either suspension or adherent cells).
<p>Fluorescence images of HeLa cells labeled with Live or Dead&trade; Cell Viability Assay Kit *Dual Fluorescence* (Cat#22788). HeLa cells at 100,000 cells/well/100 &micro;L were seeded overnight in a 96-well black wall/clear bottom plate. Cells were treated with 0-1 &micro;M staurosporine (SS) at 37&ordm;C for 4 hours (A-D), or fixed in ethanol (E), then incubated with dye-loading solution for 1 hour. The fluorescence signal was measured using a fluorescence microscope with Texas Red or Cy5 filter for viable cells (Red) and DAPI filter for necrotic cells (Blue), respectively. (F) The corresponding fluorescence signal were measured using a FlexStation&reg; microplate reader (Molecular Devices) with bottom read mode at Ex/Em= 610/650 (cutoff=630 nm, Red) and Ex/Em=360/450 (cutoff=420 nm, Blue), respectively.</p>
<p>Fluorescence images of HeLa cells labeled with Live or Dead&trade; Cell Viability Assay Kit *Dual Fluorescence* (Cat#22788). HeLa cells at 100,000 cells/well/100 &micro;L were seeded overnight in a 96-well black wall/clear bottom plate. Cells were treated with 0-1 &micro;M staurosporine (SS) at 37&ordm;C for 4 hours (A-D), or fixed in ethanol (E), then incubated with dye-loading solution for 1 hour. The fluorescence signal was measured using a fluorescence microscope with Texas Red or Cy5 filter for viable cells (Red) and DAPI filter for necrotic cells (Blue), respectively. (F) The corresponding fluorescence signal were measured using a FlexStation&reg; microplate reader (Molecular Devices) with bottom read mode at Ex/Em= 610/650 (cutoff=630 nm, Red) and Ex/Em=360/450 (cutoff=420 nm, Blue), respectively.</p>
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
22788200 Tests
Price
 
Spectral properties
Excitation (nm)612
Emission (nm)630
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12352200
Instrument settings
Fluorescence microscope
ExcitationCy5 filter (alive), DAPI filter (dead)
EmissionCy5 filter (alive), DAPI filter (dead)
Recommended plateBlack wall/clear bottom
Fluorescence microplate reader
Excitation610, 360 nm
Emission650, 450 nm
Cutoff630, 420 nm
Recommended plateBlack wall/clear bottom
Instrument specification(s)Bottom read mode
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Page updated on August 5, 2024