Name: Live or Dead™ Fixable Dead Cell Staining Kit *Blue Fluorescence with 405 nm Excitation*
Brand: AAT Bioquest
SKU: 22500
Price: 174 USD
Availability: InStock

Live or Dead™ Fixable Dead Cell Staining Kit

Blue Fluorescence with 405 nm Excitation

Our Live or Dead™ Fixable Dead Cell Staining Kits are a set of tools for labeling cells for fluorescence microscopic investigations of cellular functions. The effective labeling of cells provides a powerful method for studying cellular events in a spatial and temporal context. This particular kit is designed to uniformly label fixed mammalian cells in blue fluorescence for flow cytometry applications with violet laser excitation. The kit uses a proprietary blue fluorescent dye that is more fluorescent upon bonding to cellular components. The fluorescent dye used in the kit is well excited with the violet laser (405 nm excitation) to fluorescence at 460 nm. The kit provides all the essential components with an optimized cell-labeling protocol. It is an excellent tool for preserving of fluorescent images of particular cells, and can also be used for fluorescence flow cytometry applications.

Detection of Jurkat cell viability by Live or Dead™ Fixable Dead Cell Staining Kits (Cat#22500). Jurkat cells were treated and stained with Stain It™ Violet 450, and then fixed in 3.7% formaldehyde and analyzed by flow cytometry. Live (Red), staurosporine treated (Green) and heat-treated (Blue) cells were distinguished with Pacific Blue channel.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
22500	200 Tests	Price

Citations

View all 3 citations: Citation Explorer

Autophagy proteins are not universally required for phagosome maturation

Authors:

Cemma, Marija and Grinstein, Sergio and Brumell, John H

Journal:

Autophagy (2016): 1440--1446

Differential detection of tumor cells using a combination of cell rolling, multivalent binding, and multiple antibodies

Authors:

Myung, Ja Hye and Gajjar, Khyati A and Chen, Jihua and Molokie, Robert E and Hong, Seungpyo

Journal:

Analytical chemistry (2014): 6088--6094

Versatile fabrication of nanoscale sol--gel bioactive glass particles for efficient bone tissue regeneration

Authors:

Lei, Bo and Chen, Xiaofeng and Han, Xue and Zhou, Jiaan

Journal:

Journal of Materials Chemistry (2012): 16906--16913

References

View all 26 references: Citation Explorer

Requirements, features, and performance of high content screening platforms

Authors:

Gough AH, Johnston PA.

Journal:

Methods Mol Biol (2007): 41

A pharmaceutical company user's perspective on the potential of high content screening in drug discovery

Authors:

Hoffman AF, Garippa RJ.

Journal:

Methods Mol Biol (2007): 19

Optimizing the integration of immunoreagents and fluorescent probes for multiplexed high content screening assays

Authors:

Giuliano KA., undefined

Journal:

Methods Mol Biol (2007): 189

Past, present, and future of high content screening and the field of cellomics

Authors:

Taylor DL., undefined

Journal:

Methods Mol Biol (2007): 3

Novel fluorescent proteins for high-content screening

Authors:

Wolff M, Wiedenmann J, Nienhaus GU, Valler M, Heilker R.

Journal:

Drug Discov Today (2006): 1054

Spectral properties

Excitation (nm)	406
Emission (nm)	445

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
UNSPSC	12352200

Instrument settings

Flow cytometer
Excitation	405 nm laser
Emission	450/40 nm filter
Instrument specification(s)	Pacific Blue channel

Fluorescence microscope
Excitation	410 nm
Emission	450 nm
Recommended plate	Black wall/clear bottom

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on September 29, 2025

Component A: Stain It™ Violet 450	1 vial
Component B: DMSO	1 vial (200 µL)