Live or Dead™ Fixable Dead Cell Staining Kit *NIR Fluorescence*

Live or Dead™ Fixable Dead Cell Staining Kit

NIR Fluorescence

Our Live or Dead™ Fixable Dead Cell Staining Kits are a set of tools for labeling cells for fluorescence microscopic investigations of cellular functions. The effective labeling of cells provides a powerful method for studying cellular events in a spatial and temporal context. This particular kit is designed to uniformly label fixed mammalian cells in infrared fluorescence for flow cytometry applications with red laser excitation. The kit uses a proprietary red fluorescent dye that is more fluorescent upon binding to cellular components. The fluorescent dye used in the kit is well excited with the red (635 nm) to fluorescence at 775 nm (APC/CY7 channel). The kit provides all the essential components with an optimized cell-labeling protocol. It is an excellent tool for preserving of fluorescent images of particular cells, and can also be used for fluorescence flow cytometry applications.

Detection of Jurkat cell viability by Live or Dead™ Fixable Dead Cell Staining Kits (Cat#22605). Jurkat cells were treated and stained with Stain It™ NIR, and then fixed in 3.7% formaldehyde and analyzed by flow cytometry. Dead (Red), heat-treated (Green) and unstained (Blue) cells were distinguished with APC-Cy7 channel.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
22605	200 Tests	Price

Citations

View all 4 citations: Citation Explorer

$\alpha$$\beta$-T-cell receptor transduction gives superior mitochondrial function to $\gamma$$\delta$-T cells with promising persistence

Authors:

Ishihara, Mikiya and Miwa, Hiroshi and Fujiwara, Hiroshi and Akahori, Yasushi and Kato, Takuma and Tanaka, Yoshimasa and Tawara, Isao and Shiku, Hiroshi

Journal:

iScience (2023)

Autophagy proteins are not universally required for phagosome maturation

Authors:

Cemma, Marija and Grinstein, Sergio and Brumell, John H

Journal:

Autophagy (2016): 1440--1446

Differential detection of tumor cells using a combination of cell rolling, multivalent binding, and multiple antibodies

Authors:

Myung, Ja Hye and Gajjar, Khyati A and Chen, Jihua and Molokie, Robert E and Hong, Seungpyo

Journal:

Analytical chemistry (2014): 6088--6094

Versatile fabrication of nanoscale sol--gel bioactive glass particles for efficient bone tissue regeneration

Authors:

Lei, Bo and Chen, Xiaofeng and Han, Xue and Zhou, Jiaan

Journal:

Journal of Materials Chemistry (2012): 16906--16913

References

View all 26 references: Citation Explorer

Requirements, features, and performance of high content screening platforms

Authors:

Gough AH, Johnston PA.

Journal:

Methods Mol Biol (2007): 41

A pharmaceutical company user's perspective on the potential of high content screening in drug discovery

Authors:

Hoffman AF, Garippa RJ.

Journal:

Methods Mol Biol (2007): 19

Optimizing the integration of immunoreagents and fluorescent probes for multiplexed high content screening assays

Authors:

Giuliano KA., undefined

Journal:

Methods Mol Biol (2007): 189

Past, present, and future of high content screening and the field of cellomics

Authors:

Taylor DL., undefined

Journal:

Methods Mol Biol (2007): 3

G protein-coupled receptor internalization assays in the high-content screening format

Authors:

Haasen D, Schnapp A, Valler MJ, Heilker R.

Journal:

Methods Enzymol (2006): 121

Spectral properties

Excitation (nm)	757
Emission (nm)	779

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
UNSPSC	12352200

Instrument settings

Flow cytometer
Excitation	640 nm laser
Emission	780/60 nm filter
Instrument specification(s)	APC-Cy7 channel

Fluorescence microscope
Excitation	749 nm
Emission	775 nm
Recommended plate	Black wall/clear bottom

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 1, 2025

Component A: Stain It™ NIR fluorecence	1 vial
Component B: DMSO	1 vial (200 µL)