Name: Live or Dead™ Fixable Dead Cell Staining Kit *Deep Red Fluorescence*
Brand: AAT Bioquest
SKU: 22604
Price: 174 USD
Availability: InStock

Live or Dead™ Fixable Dead Cell Staining Kit

Deep Red Fluorescence

Our Live or Dead™ Fixable Dead Cell Staining Kits are a set of tools for labeling cells for fluorescence microscopic investigations of cell functions. The effective labeling of cells provides a powerful method for studying cellular events in a spatial and temporal context. This particular kit is designed to uniformly label fixed mammalian cells in red fluorescence for long term microscopic examination. The kit uses a proprietary red fluorescent dye that is more fluorescent upon bonding to cellular components. The fluorescent dye used in the kit is quite photostable so that the images can be repeatedly examined. The kit provides all the essential components with an optimized cell-labeling protocol. It is an excellent tool for preserving of fluorescent images of particular cells, and can also be used for fluorescence microscope demonstrations.

Detection of Jurkat cell viability by Live or Dead™ Fixable Dead Cell Staining Kits (Cat#22604). Jurkat cells were treated and stained with Stain It™ Deep Red, and then fixed in 3.7% formaldehyde and analyzed by flow cytometry. Live (Blue), staurosporine treated (Green) and heat-treated (Red) cells were distinguished with APC channel.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
22604	200 Tests	Price

Citations

View all 6 citations: Citation Explorer

Nanoparticle delivery of a pH sensitive prodrug of doxorubicin and a mitochondrial targeting VES H 8 R 8 synergistically kill multidrug resistant breast cancer cells

Authors:

Czupiel, Petro and Delplace, Vianney and Shoichet, Molly

Journal:

Scientific Reports (2020): 1--12

Nanoparticle delivery of a pH-sensitive prodrug of doxorubicin and a mitochondrial targeting VES-H 8 R 8 synergistically kill multi-drug resistant breast cancer cells

Authors:

Czupiel, Petro and Delplace, Vianney and Shoichet, Molly

Journal:

Scientific Reports (2020): 1--12

Synergistic Nanoparticle Formulations against Multi-drug Resistant Breast Cancers

Authors:

Czupiel, Petro Pawlo

Autophagy proteins are not universally required for phagosome maturation

Authors:

Cemma, Marija and Grinstein, Sergio and Brumell, John H

Journal:

Autophagy (2016): 1440--1446

Differential detection of tumor cells using a combination of cell rolling, multivalent binding, and multiple antibodies

Authors:

Myung, Ja Hye and Gajjar, Khyati A and Chen, Jihua and Molokie, Robert E and Hong, Seungpyo

Journal:

Analytical chemistry (2014): 6088--6094

References

View all 26 references: Citation Explorer

Requirements, features, and performance of high content screening platforms

Authors:

Gough AH, Johnston PA.

Journal:

Methods Mol Biol (2007): 41

A pharmaceutical company user's perspective on the potential of high content screening in drug discovery

Authors:

Hoffman AF, Garippa RJ.

Journal:

Methods Mol Biol (2007): 19

Optimizing the integration of immunoreagents and fluorescent probes for multiplexed high content screening assays

Authors:

Giuliano KA., undefined

Journal:

Methods Mol Biol (2007): 189

Past, present, and future of high content screening and the field of cellomics

Authors:

Taylor DL., undefined

Journal:

Methods Mol Biol (2007): 3

High concordance of drug-induced human hepatotoxicity with in vitro cytotoxicity measured in a novel cell-based model using high content screening

Authors:

O'Brien P J, Irwin W, Diaz D, Howard-Cofield E, Krejsa CM, Slaughter MR, Gao B, Kaludercic N, Angeline A, Bernardi P, Brain P, Hougham C.

Journal:

Arch Toxicol (2006): 580

Spectral properties

Excitation (nm)	649
Emission (nm)	664

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
UNSPSC	12352200

Instrument settings

Flow cytometer
Excitation	640 nm laser
Emission	660/20 nm filter
Instrument specification(s)	APC channel

Fluorescence microscope
Excitation	649 nm
Emission	660 nm
Recommended plate	Black wall/clear bottom

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 13, 2025

Component A: Stain It™ Deep Red	1 vial
Component B: DMSO	1 vial (200 µL)