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mFluor™ UV 375 goat anti-mouse IgG (H+L) *Cross-Absorbed*

mFluor™ UV375 goat anti-mouse conjugates are secondary antibodies designed for optimal performance in immunoassay applications, including flow cytometry, immunofluorescence, and confocal microscopy. These conjugates consist of goat-derived polyclonal antibodies with high affinity and specificity towards mouse IgG, conjugated to the bright and stable mFluor™ UV375 fluorochrome. This conjugation is optimized to ensure minimal non-specific binding and enhanced signal clarity, with rigorous purification steps to remove unconjugated components. Provided in a ready-to-use format with a recommended dilution range, the conjugate undergoes stringent quality control tests for performance and specificity. Its compatibility with a wide range of mouse primary antibodies and the exceptional contrast provided by mFluor™ UV375 fluorescence makes it a reliable tool for detecting diverse target antigens in multicolor staining protocols. mFluor™ UV375 is optimally excited by the UV laser and emits maximally at 387 nm. These affinity-purified goat anti-mouse secondary antibodies are valuable for their versatility and sensitivity, enabling efficient detection, sorting, or purification of specific targets through effective signal amplification in research applications. To minimize cross-reactivity, these goat anti-mouse IgG whole antibodies have been cross-adsorbed against human, horse, mouse, and bovine IgG.

Spectrum

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)
mFluor™ UV 375 goat anti-rabbit IgG (H+L)3513873000010.9410.0990.138
mFluor™ UV 375 goat anti-rabbit IgG (H+L) *Cross-Absorbed*3513873000010.9410.0990.138

References

View all 50 references: Citation Explorer
In-situ and amplification-free imaging of hERG ion channels at single-cell level using a unique core-molecule-shell-secondary antibody SERS nanoprobe.
Authors: Zhang, Wen-Shu and Liu, Xiao-Peng and Yue, Shuai and Wang, Ya-Ning and Wang, Yue and Xu, Zhang-Run
Journal: Talanta (2023): 123900
Use of immunoglobulin replacement therapy in patients with secondary antibody deficiency in daily practice: a European expert Q&A-based review.
Authors: Cinetto, Francesco and Francisco, Isabel Esteves and Fenchel, Klaus and Scarpa, Riccardo and Montefusco, Vittorio and Pluta, Andrzej and Wolf, Hermann M
Journal: Expert review of hematology (2023): 237-243
Recommendations for Management of Secondary Antibody Deficiency in Multiple Myeloma.
Authors: Giralt, Sergio and Jolles, Stephen and Kerre, Tessa and Lazarus, Hillard M and Mustafa, S Shahzad and Papanicolaou, Genovefa A and Ria, Roberto and Vinh, Donald C and Wingard, John R
Journal: Clinical lymphoma, myeloma & leukemia (2023): 719-732
Clinical outcomes of immunoglobulin treatment for patients with secondary antibody deficiency: Data from the Ontario immunoglobulin treatment case registry.
Authors: Abadeh, Armin and Shehadeh, Sarah and Betschel, Stephen and Waserman, Susan and Cameron, Donald William and Cowan, Juthaporn
Journal: PloS one (2023): e0294408
Development of recombinant secondary antibody mimics (rSAMs) for immunoassays through genetic fusion of monomeric alkaline phosphatase with antibody binders.
Authors: Park, Jiyeon and Bae, Yoonji and Eom, Soomin and Choi, Yuha and Lee, Giwook and Kang, Sebyung
Journal: International journal of biological macromolecules (2023): 126299
Page updated on September 19, 2024

Ordering information

Price
Unit size
200 ug
1 mg
Catalog Number
Quantity
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Additional ordering information

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Physical properties

Molecular weight

~150 kDa

Solvent

Water

Spectral properties

Absorbance (nm)

342

Correction Factor (260 nm)

0.099

Correction Factor (280 nm)

0.138

Extinction coefficient (cm -1 M -1)

300001

Excitation (nm)

351

Emission (nm)

387

Quantum yield

0.941

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501