logo
AAT Bioquest

mFluor™ UV 375 goat anti-rabbit IgG (H+L) *Cross-Absorbed*

Ordering information
Price
Catalog Number
Unit Size
Quantity
Add to cart
Additional ordering information
Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
InternationalSee distributors
Bulk requestInquire
Custom sizeInquire
ShippingStandard overnight for United States, inquire for international
Request quotation
Physical properties
Molecular weight~150 kDa
SolventWater
Spectral properties
Absorbance (nm)342
Correction Factor (260 nm)0.099
Correction Factor (280 nm)0.138
Extinction coefficient (cm -1 M -1)300001
Excitation (nm)351
Emission (nm)387
Quantum yield0.941
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
Related products
mFluor™ Violet 450 SE
mFluor™ Violet 510 SE
mFluor™ Violet 540 SE
mFluor™ Blue 570 SE
mFluor™ Green 620 SE
mFluor™ Yellow 630 SE
mFluor™ Red 700 SE
mFluor™ Red 780 SE
mFluor™ Red 780 amine
mFluor™ Violet 450-VAD-FMK
mFluor™ 510-VAD-FMK
mFluor™ Violet 450-streptavidin conjugate
mFluor™ Violet 510-streptavidin conjugate
mFluor™ Violet 540-streptavidin conjugate
mFluor™ Blue 570-streptavidin conjugate
mFluor™ Green 620-streptavidin conjugate
mFluor™ Yellow 630-streptavidin conjugate
mFluor™ Red 700-streptavidin conjugate
mFluor™ Red 780-streptavidin conjugate
mFluor™ Violet 450-dUTP *1 mM in Tris Buffer (pH 7.5)*
mFluor™ Violet 450 acid
mFluor™ Violet 510 acid
mFluor™ Violet 540 acid
mFluor™ Blue 570 acid
mFluor™ Green 620 acid
mFluor™ Yellow 630 acid
mFluor™ Red 700 acid
mFluor™ Violet 500 SE
mFluor™ UV375 SE
mFluor™ Red 780 acid
mFluor™ UV460 SE
mFluor™ Blue 630 SE
mFluor™ Blue 660 SE
mFluor™ Violet 610 SE
mFluor™ Green 630 SE
mFluor™ Blue 580 SE
mFluor™ Blue 590 SE
mFluor™ Blue 620 SE
mFluor™ Red 780 Maleimide
mFluor™ Violet 550 SE
mFluor™ Violet 505 SE
mFluor™ Violet 590 SE
mFluor™ Violet 545 SE
mFluor™ UV420 SE
mFluor™ UV455 SE
mFluor™ UV520 SE
mFluor™ UV540 SE
mFluor™ UV610 SE
mFluor™ Violet 450 Azide
mFluor™ Violet 450 maleimide
mFluor™ UV 375 Biotin Conjugate
mFluor™ UV 460 Biotin Conjugate
mFluor™ Violet 500 Biotin Conjugate
mFluor™ Violet 540 Biotin Conjugate
mFluor™ Red 780 Biotin Conjugate
mFluor™ Violet 530 SE
mFluor™ Violet 530 maleimide
mFluor™ Violet 480 SE
mFluor™ Blue 660 tyramide
mFluor™ Violet 450-PEG4-Biotin Conjugate
mFluor™ Violet 450-Wheat Germ Agglutinin (WGA) Conjugate
mFluor™ Violet 500-Wheat Germ Agglutinin (WGA) Conjugate
mFluor™ Violet 540-Wheat Germ Agglutinin (WGA) Conjugate
mFluor™ Blue 585 SE
mFluor™ Blue 583 SE
mFluor™ Blue 580 Styramide
mFluor™ Blue 630 Styramide
mFluor™ Blue 660 Styramide
mFluor™ Green 620 Styramide
mFluor™ Red 780 Styramide
mFluor™ Violet 540 Styramide
mFluor™ Violet 545 Styramide
mFluor™ Violet 610 Styramide
mFluor™ UV455-streptavidin conjugate
mFluor™ Violet 545-streptavidin conjugate
mFluor™ Violet 550-streptavidin conjugate
mFluor™ Violet 590-streptavidin conjugate
mFluor™ Violet 610-streptavidin conjugate
mFluor™ Violet 510 goat anti-mouse IgG (H+L)
mFluor™ Violet 510 goat anti-mouse IgG (H+L) *Cross-Absorbed*
mFluor™ Violet 510 goat anti-rabbit IgG (H+L)
mFluor™ Violet 510 goat anti-rabbit IgG (H+L) *Cross-Absorbed*
mFluor™ Red 780 goat anti-rabbit IgG (H+L) *Cross-Absorbed*
mFluor™ Red 780 goat anti-rabbit IgG (H+L)
mFluor™ Red 780 goat anti-mouse IgG (H+L) *Cross-Absorbed*
mFluor™ Red 780 goat anti-mouse IgG (H+L)
mFluor™ Blue 585 Anti-human CD4 Antibody *SK3*
mFluor™ UV420-streptavidin conjugate
mFluor™ Blue 615 SE
mFluor™ Green 615 SE
Show More (80)

OverviewpdfSDSpdfProtocol


Molecular weight
~150 kDa
Absorbance (nm)
342
Correction Factor (260 nm)
0.099
Correction Factor (280 nm)
0.138
Extinction coefficient (cm -1 M -1)
300001
Excitation (nm)
351
Emission (nm)
387
Quantum yield
0.941
mFluor™ UV375 goat anti-rabbit conjugates are secondary antibodies designed for optimal performance in immunoassay applications, including flow cytometry, immunofluorescence, and confocal microscopy. These conjugates consist of goat-derived polyclonal antibodies with high affinity and specificity towards rabbit IgG, conjugated to the bright and stable mFluor™ UV375 fluorochrome. This conjugation is optimized to ensure minimal non-specific binding and enhanced signal clarity, with rigorous purification steps to remove unconjugated components. Provided in a ready-to-use format with a recommended dilution range, the conjugate undergoes stringent quality control tests for performance and specificity. Its compatibility with a wide range of rabbit primary antibodies and the exceptional contrast provided by mFluor™ UV375 fluorescence makes it a reliable tool for detecting diverse target antigens in multicolor staining protocols. mFluor™ UV375 is optimally excited by the UV laser and emits maximally at 387 nm. These affinity-purified goat anti-rabbit secondary antibodies are valuable for their versatility and sensitivity, enabling efficient detection, sorting, or purification of specific targets through effective signal amplification in research applications. To minimize cross-reactivity, these goat anti-mouse IgG whole antibodies have been cross-adsorbed against human, horse, mouse, and bovine IgG.

Spectrum


Open in Advanced Spectrum Viewer
spectrum

Spectral properties

Absorbance (nm)342
Correction Factor (260 nm)0.099
Correction Factor (280 nm)0.138
Extinction coefficient (cm -1 M -1)300001
Excitation (nm)351
Emission (nm)387
Quantum yield0.941

Product Family


NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)
mFluor™ UV 375 goat anti-mouse IgG (H+L)3513873000010.9410.0990.138
mFluor™ UV 375 goat anti-mouse IgG (H+L) *Cross-Absorbed*3513873000010.9410.0990.138

References


View all 5 references: Citation Explorer
FRET causing misleading signal from fluorescein excited by the violet laser in flow cytometry.
Authors: Waeckel, Louis and Khenine, Hana and Berger, Anne-Emmanuelle and Lambert, Claude
Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2023)
A ten-color tube with dried antibody reagents for the screening of hematological malignancies.
Authors: Correia, R P and Rajab, A and Bento, L C and Alexandre, A M and Vaz, A C and Schimidell, D and Pedro, E C and Perin, F S and Nozawa, S T and Barroso, R S and Bacal, N S
Journal: International journal of laboratory hematology (2018): 136-143
[THE STANDARD VALUES OF SUB-POPULATIONS OF T-HELPERS OF DIFFERENT LEVEL OF DIFFERENTIATION IN PERIPHERAL BLOOD].
Authors: Kudryavtsev, I V and Serebryakova, M K and Totolyan, A A
Journal: Klinicheskaia laboratornaia diagnostika (2016): 179-84
Quantification of mitochondrial reactive oxygen species in living cells by using multi-laser polychromatic flow cytometry.
Authors: De Biasi, Sara and Gibellini, Lara and Bianchini, Elena and Nasi, Milena and Pinti, Marcello and Salvioli, Stefano and Cossarizza, Andrea
Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2016): 1106-1110
Tyramide signal amplification for analysis of kinase activity by intracellular flow cytometry.
Authors: Clutter, Matthew R and Heffner, Garrett C and Krutzik, Peter O and Sachen, Kacey L and Nolan, Garry P
Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2010): 1020-31