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mFluor™ UV 375 goat anti-rabbit IgG (H+L) *Cross-Absorbed*

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Fax1-800-609-2943
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Physical properties
Molecular weight~150 kDa
SolventWater
Spectral properties
Absorbance (nm)342
Correction Factor (260 nm)0.099
Correction Factor (280 nm)0.138
Extinction coefficient (cm -1 M -1)300001
Excitation (nm)351
Emission (nm)387
Quantum yield0.941
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
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OverviewpdfSDSpdfProtocol


Molecular weight
~150 kDa
Absorbance (nm)
342
Correction Factor (260 nm)
0.099
Correction Factor (280 nm)
0.138
Extinction coefficient (cm -1 M -1)
300001
Excitation (nm)
351
Emission (nm)
387
Quantum yield
0.941
mFluor™ UV375 goat anti-rabbit conjugates are secondary antibodies designed for optimal performance in immunoassay applications, including flow cytometry, immunofluorescence, and confocal microscopy. These conjugates consist of goat-derived polyclonal antibodies with high affinity and specificity towards rabbit IgG, conjugated to the bright and stable mFluor™ UV375 fluorochrome. This conjugation is optimized to ensure minimal non-specific binding and enhanced signal clarity, with rigorous purification steps to remove unconjugated components. Provided in a ready-to-use format with a recommended dilution range, the conjugate undergoes stringent quality control tests for performance and specificity. Its compatibility with a wide range of rabbit primary antibodies and the exceptional contrast provided by mFluor™ UV375 fluorescence makes it a reliable tool for detecting diverse target antigens in multicolor staining protocols. mFluor™ UV375 is optimally excited by the UV laser and emits maximally at 387 nm. These affinity-purified goat anti-rabbit secondary antibodies are valuable for their versatility and sensitivity, enabling efficient detection, sorting, or purification of specific targets through effective signal amplification in research applications. To minimize cross-reactivity, these goat anti-mouse IgG whole antibodies have been cross-adsorbed against human, horse, mouse, and bovine IgG.

Spectrum


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spectrum

Spectral properties

Absorbance (nm)342
Correction Factor (260 nm)0.099
Correction Factor (280 nm)0.138
Extinction coefficient (cm -1 M -1)300001
Excitation (nm)351
Emission (nm)387
Quantum yield0.941

Product Family


NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)
mFluor™ UV 375 goat anti-mouse IgG (H+L)3513873000010.9410.0990.138
mFluor™ UV 375 goat anti-mouse IgG (H+L) *Cross-Absorbed*3513873000010.9410.0990.138

References


View all 5 references: Citation Explorer
FRET causing misleading signal from fluorescein excited by the violet laser in flow cytometry.
Authors: Waeckel, Louis and Khenine, Hana and Berger, Anne-Emmanuelle and Lambert, Claude
Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2023)
A ten-color tube with dried antibody reagents for the screening of hematological malignancies.
Authors: Correia, R P and Rajab, A and Bento, L C and Alexandre, A M and Vaz, A C and Schimidell, D and Pedro, E C and Perin, F S and Nozawa, S T and Barroso, R S and Bacal, N S
Journal: International journal of laboratory hematology (2018): 136-143
Quantification of mitochondrial reactive oxygen species in living cells by using multi-laser polychromatic flow cytometry.
Authors: De Biasi, Sara and Gibellini, Lara and Bianchini, Elena and Nasi, Milena and Pinti, Marcello and Salvioli, Stefano and Cossarizza, Andrea
Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2016): 1106-1110
[THE STANDARD VALUES OF SUB-POPULATIONS OF T-HELPERS OF DIFFERENT LEVEL OF DIFFERENTIATION IN PERIPHERAL BLOOD].
Authors: Kudryavtsev, I V and Serebryakova, M K and Totolyan, A A
Journal: Klinicheskaia laboratornaia diagnostika (2016): 179-84
Tyramide signal amplification for analysis of kinase activity by intracellular flow cytometry.
Authors: Clutter, Matthew R and Heffner, Garrett C and Krutzik, Peter O and Sachen, Kacey L and Nolan, Garry P
Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2010): 1020-31