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mFluor™ UV 375 goat anti-mouse IgG (H+L)

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Physical properties
Molecular weight~150 kDa
SolventWater
Spectral properties
Absorbance (nm)342
Correction Factor (260 nm)0.099
Correction Factor (280 nm)0.138
Extinction coefficient (cm -1 M -1)300001
Excitation (nm)351
Emission (nm)387
Quantum yield0.941
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
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OverviewpdfSDSpdfProtocol


Molecular weight
~150 kDa
Absorbance (nm)
342
Correction Factor (260 nm)
0.099
Correction Factor (280 nm)
0.138
Extinction coefficient (cm -1 M -1)
300001
Excitation (nm)
351
Emission (nm)
387
Quantum yield
0.941
mFluor™ UV375 goat anti-mouse conjugates are secondary antibodies designed for optimal performance in immunoassay applications, including flow cytometry, immunofluorescence, and confocal microscopy. These conjugates consist of goat-derived polyclonal antibodies with high affinity and specificity towards mouse IgG, conjugated to the bright and stable mFluor™ UV375 fluorochrome. This conjugation is optimized to ensure minimal non-specific binding and enhanced signal clarity, with rigorous purification steps to remove unconjugated components. Provided in a ready-to-use format with a recommended dilution range, the conjugate undergoes stringent quality control tests for performance and specificity. Its compatibility with a wide range of mouse primary antibodies and the exceptional contrast provided by mFluor™ UV375 fluorescence makes it a reliable tool for detecting diverse target antigens in multicolor staining protocols. mFluor™ UV375 is optimally excited by the UV laser and emits maximally at 387 nm. These affinity-purified goat anti-mouse secondary antibodies are valuable for their versatility and sensitivity, enabling efficient detection, sorting, or purification of specific targets through effective signal amplification in research applications.

Spectrum


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spectrum

Spectral properties

Absorbance (nm)342
Correction Factor (260 nm)0.099
Correction Factor (280 nm)0.138
Extinction coefficient (cm -1 M -1)300001
Excitation (nm)351
Emission (nm)387
Quantum yield0.941

Product Family


NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)
mFluor™ UV 375 goat anti-rabbit IgG (H+L)3513873000010.9410.0990.138
mFluor™ UV 375 goat anti-rabbit IgG (H+L) *Cross-Absorbed*3513873000010.9410.0990.138

References


View all 49 references: Citation Explorer
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Journal: Food chemistry (2024): 137704
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Journal: Romanian journal of morphology and embryology = Revue roumaine de morphologie et embryologie (2023): 189-197
A very rare cause of blue finger: A case-based review.
Authors: Hassan, Fadi and Khoury, Amir and Awad, Jamal and Jeries, Helana and Naffaa, Mohammad E
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Journal: Bioelectrochemistry (Amsterdam, Netherlands) (2023): 108431
Construction of a competitive electrochemical immunosensor based on sacrifice of Prussian blue and its ultrasensitive detection of alpha-fetoprotein.
Authors: Wang, Min and Jiang, Mingzhe and Li, Pengli and Yuan, Mengting and Zhao, Chulei and Lai, Wenjing and Li, Jiajia and Hong, Chenglin and Qi, Yu
Journal: Analytica chimica acta (2023): 341143
Novel Bimolecular Fluorescence Complementation (BiFC) Assay for Visualization of the Protein-Protein Interactions and Cellular Protein Complex Localizations.
Authors: Shi, Zhonggang and Gao, Xing and Zhang, Wenrui and Chen, Binghong and Wang, Mengying and Liao, Keman and Wang, Zhihan and Ren, Li and Zhai, Yujia and Qiu, Yongming and Wang, Xuhui and Lin, Yingying
Journal: Molecular biotechnology (2023)
Carbon quantum dot fluorescent probe for labeling and imaging of stellate cell on liver frozen section below freezing point.
Authors: He, Yan-Fei and Cheng, Kai and Zhong, Zi-Tao and Hou, Xiao-Lin and An, Chang-Zhi and Zhang, Jing and Chen, Wei and Liu, Bo and Yuan, Jing and Zhao, Yuan-Di
Journal: Analytica chimica acta (2023): 341210
A sandwich electrochemical immunosensor based on MXene@dual MOFs for detection of tumor marker CA125.
Authors: Qu, Lingli and Wu, Mengdie and Zhao, Lu and Li, Jiang and Pan, Hongzhi
Journal: Mikrochimica acta (2023): 147
LONG-TERM FOLLOW-UP OF ASYMMETRIC BILATERAL DIFFUSE UVEAL MELANOCYTIC PROLIFERATION IN A PATIENT WITH METASTASIZED UROTHELIAL CARCINOMA.
Authors: Lentzsch, Anna M and Siggel, Robert and Schnorr, Christel and Holtick, Udo and Liakopoulos, Sandra
Journal: Retinal cases & brief reports (2023): 105-110
A Novel Enzyme-Free Ratiometric Fluorescence Immunoassay Based on Silver Nanoparticles for the Detection of Dibutyl Phthalate from Environmental Waters.
Authors: Meng, Hui and Yao, Nannan and Zeng, Kun and Zhu, Nuanfei and Wang, Yue and Zhao, Biying and Zhang, Zhen
Journal: Biosensors (2022)