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mFluor™ UV 375 goat anti-mouse IgG (H+L)

mFluor™ UV375 goat anti-mouse conjugates are secondary antibodies designed for optimal performance in immunoassay applications, including flow cytometry, immunofluorescence, and confocal microscopy. These conjugates consist of goat-derived polyclonal antibodies with high affinity and specificity towards mouse IgG, conjugated to the bright and stable mFluor™ UV375 fluorochrome. This conjugation is optimized to ensure minimal non-specific binding and enhanced signal clarity, with rigorous purification steps to remove unconjugated components. Provided in a ready-to-use format with a recommended dilution range, the conjugate undergoes stringent quality control tests for performance and specificity. Its compatibility with a wide range of mouse primary antibodies and the exceptional contrast provided by mFluor™ UV375 fluorescence makes it a reliable tool for detecting diverse target antigens in multicolor staining protocols. mFluor™ UV375 is optimally excited by the UV laser and emits maximally at 387 nm. These affinity-purified goat anti-mouse secondary antibodies are valuable for their versatility and sensitivity, enabling efficient detection, sorting, or purification of specific targets through effective signal amplification in research applications.

Spectrum

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)
mFluor™ UV 375 goat anti-rabbit IgG (H+L)3513873000010.9410.0990.138
mFluor™ UV 375 goat anti-rabbit IgG (H+L) *Cross-Absorbed*3513873000010.9410.0990.138

References

View all 49 references: Citation Explorer
A novel sandwich-type electrochemical immunosensor for sensitive detection of zearalenone using NG/PDDA/HNTs and Ti-MOF-KB composites for signal amplification.
Authors: Jiang, Xiaodan and Mu, Zhaode and Wang, Jie and Zhou, Jing and Bai, Lijuan
Journal: Food chemistry (2024): 137704
A sandwich electrochemical immunosensor based on MXene@dual MOFs for detection of tumor marker CA125.
Authors: Qu, Lingli and Wu, Mengdie and Zhao, Lu and Li, Jiang and Pan, Hongzhi
Journal: Mikrochimica acta (2023): 147
LONG-TERM FOLLOW-UP OF ASYMMETRIC BILATERAL DIFFUSE UVEAL MELANOCYTIC PROLIFERATION IN A PATIENT WITH METASTASIZED UROTHELIAL CARCINOMA.
Authors: Lentzsch, Anna M and Siggel, Robert and Schnorr, Christel and Holtick, Udo and Liakopoulos, Sandra
Journal: Retinal cases & brief reports (2023): 105-110
Carbon quantum dot fluorescent probe for labeling and imaging of stellate cell on liver frozen section below freezing point.
Authors: He, Yan-Fei and Cheng, Kai and Zhong, Zi-Tao and Hou, Xiao-Lin and An, Chang-Zhi and Zhang, Jing and Chen, Wei and Liu, Bo and Yuan, Jing and Zhao, Yuan-Di
Journal: Analytica chimica acta (2023): 341210
Novel Bimolecular Fluorescence Complementation (BiFC) Assay for Visualization of the Protein-Protein Interactions and Cellular Protein Complex Localizations.
Authors: Shi, Zhonggang and Gao, Xing and Zhang, Wenrui and Chen, Binghong and Wang, Mengying and Liao, Keman and Wang, Zhihan and Ren, Li and Zhai, Yujia and Qiu, Yongming and Wang, Xuhui and Lin, Yingying
Journal: Molecular biotechnology (2023)
Page updated on October 9, 2024

Ordering information

Price
Unit size
200 ug
1 mg
Catalog Number
Quantity
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Additional ordering information

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Physical properties

Molecular weight

~150 kDa

Solvent

Water

Spectral properties

Absorbance (nm)

342

Correction Factor (260 nm)

0.099

Correction Factor (280 nm)

0.138

Extinction coefficient (cm -1 M -1)

300001

Excitation (nm)

351

Emission (nm)

387

Quantum yield

0.941

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501