mFluor™ UV460 SE

AAT Bioquest's mFluor™ dyes are developed for multicolor flow cytometry-focused applications. These dyes have large Stokes Shifts, and can be well excited by the laser lines of flow cytometers (e.g., 355 nm, 405 nm, 488 nm and 633 nm). mFluor™ UV460 (MFUV460) dyes are well excited by UV excitation with emission at ~460 nm. MFUV460 has the spectral properties almost identical to those of Marina Blue® dye. These spectral characteristics make them an excellent alternative to Marina Blue® dye. Marina Blue® is the trademark of ThermoFisher.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
1136	1 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of mFluor™ UV460 SE to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	272.287 µL	1.361 mL	2.723 mL	13.614 mL	27.229 mL
5 mM	54.457 µL	272.287 µL	544.573 µL	2.723 mL	5.446 mL
10 mM	27.229 µL	136.143 µL	272.287 µL	1.361 mL	2.723 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	367.26 g/mol	=	mL	x	mM	=	mmol

Citations

View all 11 citations: Citation Explorer

Homo- and hetero-oligomerization of hydrophobic pulmonary surfactant proteins SP-B and SP-C in surfactant phospholipid membranes

Authors:

Cabre, E. J., Martinez-Calle, M., Prieto, M., Fedorov, A., Olmeda, B., Loura, L. M. S., Perez-Gil, J.

Journal:

J Biol Chem (2018): 9399-9411

Molecular characterization of DXCF cyanobacteriochromes from the cyanobacterium Acaryochloris marina identifies a blue-light power sensor

Authors:

Hasegawa, M., Fushimi, K., Miyake, K., Nakajima, T., Oikawa, Y., Enomoto, G., Sato, M., Ikeuchi, M., Narikawa, R.

Journal:

J Biol Chem (2018): 1713-1727

Low blue carbon storage in eelgrass (Zostera marina) meadows on the Pacific Coast of Canada

Authors:

Postlethwaite, V. R., McGowan, A. E., Kohfeld, K. E., Robinson, C. L. K., Pellatt, M. G.

Journal:

PLoS One (2018): e0198348

Simultaneous lipid and content mixing assays for in vitro reconstitution studies of synaptic vesicle fusion

Authors:

Liu, X., Seven, A. B., Xu, J., Esser, V., Su, L., Ma, C., Rizo, J.

Journal:

Nat Protoc (2017): 2014-2028

A biliverdin-binding cyanobacteriochrome from the chlorophyll d-bearing cyanobacterium Acaryochloris marina

Authors:

Narikawa, R., Nakajima, T., Aono, Y., Fushimi, K., Enomoto, G., Ni Ni, Win, Itoh, S., Sato, M., Ikeuchi, M.

Journal:

Sci Rep (2015): 7950

Physical properties

Molecular weight	367.26
Solvent	DMSO

Spectral properties

Absorbance (nm)	364
Correction factor (260 nm)	0.35
Correction factor (280 nm)	0.134
Extinction coefficient (cm ^-1 M ^-1)	15000 ¹
Excitation (nm)	358
Emission (nm)	456
Quantum yield	0.86 ¹

Storage, safety and handling

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

Documents

Protocol
Safety Data Sheet
Certificate of Analysis

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Page updated on October 24, 2025