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mFluor™ UV520 SE
mFluor™ dyes are developed for multicolor flow cytometry-focused applications. These dyes have large Stokes Shifts, and can be well excited by the laser lines of flow cytometers (e.g., 350 nm, 405 nm, 488 nm and 633 nm). mFluor™ UV dyes are optimized to be excited with a UV laser at 350 nm. AAT Bioquest offers the largest collection of fluorescent dyes that are excited by UV laser at 350 nm. mFluor™ UV 520 dyes have fluorescence excitation and emission maxima of ~350 nm and ~520 nm respectively. These spectral characteristics make them a unique color for flow cytometry application. mFluor™ UV 520 SE is reasonably stable and shows good reactivity and selectivity with protein amino groups. mFluor™ UV 520 SE provides a convenient tool to label monoclonal, polyclonal antibodies or other proteins (>10 kDa) for flow cytometric applications with the UV laser excitation.
Fluorescent dye NHS esters (or succinimidyl esters) are the most popular tool for conjugating dyes to a peptide, protein, antibody, amino-modified oligonucleotide, or nucleic acid. NHS esters react readily with the primary amines (R-NH<sub>2</sub>) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. The resulting dye conjugates are quite stable.
Fluorescent dye NHS esters (or succinimidyl esters) are the most popular tool for conjugating dyes to a peptide, protein, antibody, amino-modified oligonucleotide, or nucleic acid. NHS esters react readily with the primary amines (R-NH<sub>2</sub>) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. The resulting dye conjugates are quite stable.
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
16431 mg
Price
 
Physical properties
Molecular weight1531.85
SolventDMSO
Spectral properties
Absorbance (nm)502
Correction factor (260 nm)0.495
Correction factor (280 nm)0.518
Extinction coefficient (cm -1 M -1)
80000
1
Excitation (nm)367
Emission (nm)524
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
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Page updated on October 15, 2025