mFluor™ Violet 450 Azide
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
| Quantity |
Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Molecular weight | 610.59 |
| Solvent | DMSO |
Spectral properties
| Absorbance (nm) | 406 |
| Correction Factor (260 nm) | 0.338 |
| Correction Factor (280 nm) | 0.078 |
| Extinction coefficient (cm -1 M -1) | 350001 |
| Excitation (nm) | 406 |
| Emission (nm) | 445 |
| Quantum yield | 0.811 |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
| UNSPSC | 12171501 |
Alternative formats
| mFluor™ Violet 450 maleimide |
| mFluor™ Violet 450 acid |
| Overview |  SDSProtocol |
See also: mFluor™ Dyes and Kits
Molecular weight 610.59 | Absorbance (nm) 406 | Correction Factor (260 nm) 0.338 | Correction Factor (280 nm) 0.078 | Extinction coefficient (cm -1 M -1) 350001 | Excitation (nm) 406 | Emission (nm) 445 | Quantum yield 0.811 |
AAT Bioquest's mFluor™ dyes are developed for multicolor flow cytometry-focused applications. These dyes have large Stokes Shifts, and can be well excited by the laser lines of flow cytometers (e.g., 405 nm, 488 nm and 633 nm). mFluor™ Violet 450 dyes have fluorescence excitation and emission maxima of ~405 nm and ~450 nm respectively. These spectral characteristics make them an excellent replacement for Pacific Blue™ labeling dye. mFluor™ Violet 450 azide is quite stable and shows good reactivity and selectivity with an alkyne group. mFluor™ Violet 450 azide provides a convenient tool to label an alkyne-modified biomolecule.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of mFluor™ Violet 450 Azide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 163.776 µL | 818.88 µL | 1.638 mL | 8.189 mL | 16.378 mL |
| 5 mM | 32.755 µL | 163.776 µL | 327.552 µL | 1.638 mL | 3.276 mL |
| 10 mM | 16.378 µL | 81.888 µL | 163.776 µL | 818.88 µL | 1.638 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
| / | = | x | = |
Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
| Absorbance (nm) | 406 |
| Correction Factor (260 nm) | 0.338 |
| Correction Factor (280 nm) | 0.078 |
| Extinction coefficient (cm -1 M -1) | 350001 |
| Excitation (nm) | 406 |
| Emission (nm) | 445 |
| Quantum yield | 0.811 |
Product Family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
| mFluor™ Violet 450 SE | 406 | 445 | 350001 | 0.811 | 0.338 | 0.078 |
| mFluor™ Violet 450-dUTP *1 mM in Tris Buffer (pH 7.5)* | 406 | 445 | 350001 | 0.811 | 0.338 | 0.078 |
Images
Figure 1. Chemical structure for mFluor™ Violet 450 Azide.
References
View all 2 references: Citation Explorer
Development of dual-color total internal reflection fluorescence biosensor for simultaneous quantitation of two small molecules and their affinity constants with antibodies.
Authors: Song, Dan and Yang, Rong and Wang, Hongliang and Fang, Sunyan and Liu, Yanping and Long, Feng and Zhu, Anna
Journal: Biosensors & bioelectronics (2019): 824-830
Authors: Song, Dan and Yang, Rong and Wang, Hongliang and Fang, Sunyan and Liu, Yanping and Long, Feng and Zhu, Anna
Journal: Biosensors & bioelectronics (2019): 824-830
A novel dual-color total internal reflection fluorescence detecting platform using compact optical structure and silicon-based photodetector.
Authors: Song, Dan and Yang, Rong and Fang, Shunyan and Liu, Yanping and Liu, Jiayao and Xu, Wenjuan and Long, Feng and Zhu, Anna
Journal: Talanta (2019): 78-84
Authors: Song, Dan and Yang, Rong and Fang, Shunyan and Liu, Yanping and Liu, Jiayao and Xu, Wenjuan and Long, Feng and Zhu, Anna
Journal: Talanta (2019): 78-84