mFluor™ Violet 450 Azide

AAT Bioquest's mFluor™ dyes are developed for multicolor flow cytometry-focused applications. These dyes have large Stokes Shifts, and can be well excited by the laser lines of flow cytometers (e.g., 405 nm, 488 nm and 633 nm). mFluor™ Violet 450 dyes have fluorescence excitation and emission maxima of ~405 nm and ~450 nm respectively. These spectral characteristics make them an excellent replacement for Pacific Blue™ labeling dye. mFluor™ Violet 450 azide is quite stable and shows good reactivity and selectivity with an alkyne group. mFluor™ Violet 450 azide provides a convenient tool to label an alkyne-modified biomolecule.

Spectrum

Open in Advanced Spectrum Viewer

Product family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Quantum yield	Correction Factor (260 nm)	Correction Factor (280 nm)
mFluor™ Violet 450-dUTP 1 mM in TE Buffer (pH 7.5)	406	445	35000¹	0.81¹	0.338	0.078
mFluor™ Violet 450 SE	406	445	35000¹	0.81¹	0.338	0.078

References

View all 2 references: Citation Explorer

A novel dual-color total internal reflection fluorescence detecting platform using compact optical structure and silicon-based photodetector.
Authors: Song, Dan and Yang, Rong and Fang, Shunyan and Liu, Yanping and Liu, Jiayao and Xu, Wenjuan and Long, Feng and Zhu, Anna
Journal: Talanta (2019): 78-84

Development of dual-color total internal reflection fluorescence biosensor for simultaneous quantitation of two small molecules and their affinity constants with antibodies.
Authors: Song, Dan and Yang, Rong and Wang, Hongliang and Fang, Sunyan and Liu, Yanping and Long, Feng and Zhu, Anna
Journal: Biosensors & bioelectronics (2019): 824-830

Page updated on May 13, 2025

Ordering information

Price
Unit size
Catalog Number	1690
Quantity

Add to cart

Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Request quotation

Physical properties

Molecular weight	610.59
Solvent	DMSO

Spectral properties

Absorbance (nm)	406
Correction Factor (260 nm)	0.338
Correction Factor (280 nm)	0.078
Extinction coefficient (cm ^-1 M ^-1)	35000¹
Excitation (nm)	406
Emission (nm)	445
Quantum yield	0.81¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501