mFluor™ Violet 450 Azide

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Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
Quotation	Request
International	See distributors
Shipping	Standard overnight for United States, inquire for international

Physical properties

Molecular weight	610.59
Solvent	DMSO

Spectral properties

Absorbance (nm)	406
Correction Factor (260 nm)	0.338
Correction Factor (280 nm)	0.078
Extinction coefficient (cm ^-1 M ^-1)	35000¹
Excitation (nm)	406
Emission (nm)	445
Quantum yield	0.81¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Alternative formats

mFluor™ Violet 450 maleimide

mFluor™ Violet 450 acid

Overview SDS Protocol

Molecular weight

610.59

Absorbance (nm)

406

Correction Factor (260 nm)

0.338

Correction Factor (280 nm)

0.078

Extinction coefficient (cm ^-1 M ^-1)

35000¹

Excitation (nm)

406

Emission (nm)

445

Quantum yield

0.81¹

AAT Bioquest's mFluor™ dyes are developed for multicolor flow cytometry-focused applications. These dyes have large Stokes Shifts, and can be well excited by the laser lines of flow cytometers (e.g., 405 nm, 488 nm and 633 nm). mFluor™ Violet 450 dyes have fluorescence excitation and emission maxima of ~405 nm and ~450 nm respectively. These spectral characteristics make them an excellent replacement for Pacific Blue™ labeling dye. mFluor™ Violet 450 azide is quite stable and shows good reactivity and selectivity with an alkyne group. mFluor™ Violet 450 azide provides a convenient tool to label an alkyne-modified biomolecule.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of mFluor™ Violet 450 Azide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	163.776 µL	818.88 µL	1.638 mL	8.189 mL	16.378 mL
5 mM	32.755 µL	163.776 µL	327.552 µL	1.638 mL	3.276 mL
10 mM	16.378 µL	81.888 µL	163.776 µL	818.88 µL	1.638 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
	/		=		x		=

Spectrum

Open in Advanced Spectrum Viewer

Spectral properties

Absorbance (nm)	406
Correction Factor (260 nm)	0.338
Correction Factor (280 nm)	0.078
Extinction coefficient (cm ^-1 M ^-1)	35000¹
Excitation (nm)	406
Emission (nm)	445
Quantum yield	0.81¹

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Quantum yield	Correction Factor (260 nm)	Correction Factor (280 nm)
mFluor™ Violet 450 SE	406	445	35000¹	0.81¹	0.338	0.078
mFluor™ Violet 450-dUTP 1 mM in Tris Buffer (pH 7.5)	406	445	35000¹	0.81¹	0.338	0.078

Images

Figure 1. Chemical structure for mFluor™ Violet 450 Azide.

References

View all 2 references: Citation Explorer

Development of dual-color total internal reflection fluorescence biosensor for simultaneous quantitation of two small molecules and their affinity constants with antibodies.
Authors: Song, Dan and Yang, Rong and Wang, Hongliang and Fang, Sunyan and Liu, Yanping and Long, Feng and Zhu, Anna
Journal: Biosensors & bioelectronics (2019): 824-830

A novel dual-color total internal reflection fluorescence detecting platform using compact optical structure and silicon-based photodetector.
Authors: Song, Dan and Yang, Rong and Fang, Shunyan and Liu, Yanping and Liu, Jiayao and Xu, Wenjuan and Long, Feng and Zhu, Anna
Journal: Talanta (2019): 78-84