Name: mFluor™ Violet 450-dUTP *1 mM in TE Buffer (pH 7.5)*
Brand: AAT Bioquest
SKU: 17011
Price: 348 USD
Availability: InStock

mFluor™ Violet 450-dUTP

1 mM in TE Buffer (pH 7.5)

The dye-modified deoxyuridine 5'-triphosphates (such as aminoallyl-dUTP) can be used to produce dye-containing DNA by conventional enzymatic incorporation methods such as reverse transcription, nick translation, random primed labeling, or PCR. This enzymatic fluorescence labeling method is widely used for both FISH probes and microarray-based experiments. This mFluor™ 450-dUTP conjugate can be used as a blue fluorescence color with Pacific Blue® filter set. (Pacific Blue® is the trademark of Invitrogen).

Chemical structure for mFluor™ Violet 450-dUTP *1 mM in Tris Buffer (pH 7.5)*

Protocol

SDS

COA

Catalog	Size	Price	Quantity
17011	25 nmoles	Price

References

View all 9 references: Citation Explorer

A visual DNA chip for simultaneous detection of hepatitis B virus, hepatitis C virus and human immunodeficiency virus type-1

Authors:

Wen JK, Zhang XE, Cheng Z, Liu H, Zhou YF, Zhang ZP, Yang JH, Deng JY.

Journal:

Biosens Bioelectron (2004): 685

Fluorescent DNA hybridization probe preparation using amine modification and reactive dye coupling

Authors:

Cox WG, Singer VL.

Journal:

Biotechniques (2004): 114

Aminomodified nucleobases: functionalized nucleoside triphosphates applicable for SELEX

Authors:

Schoetzau T, Langner J, Moyroud E, Roehl I, Vonhoff S, Klussmann S.

Journal:

Bioconjug Chem (2003): 919

Topology of yeast RNA polymerase II subunits in transcription elongation complexes studied by photoaffinity cross-linking

Authors:

Wooddell CI, Burgess RR.

Journal:

Biochemistry (2000): 13405

Simple method for preparation of fluor/hapten-labeled dUTP

Authors:

Nimmakayalu M, Henegariu O, Ward DC, Bray-Ward P.

Journal:

Biotechniques (2000): 518

Physical properties

Molecular weight	~1000
Solvent	Water

Spectral properties

Absorbance (nm)	406
Correction factor (260 nm)	0.338
Correction factor (280 nm)	0.078
Extinction coefficient (cm ^-1 M ^-1)	35000 ¹
Excitation (nm)	406
Emission (nm)	445
Quantum yield	0.81 ¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure

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