logo
AAT Bioquest

mFluor™ Violet 540 acid

With EDAC or other equivalent activating coupling agents, fluorescent dyes can react readily with the primary amines (R-NH2) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. The resulting dye conjugates are quite stable.
With EDAC or other equivalent activating coupling agents, fluorescent dyes can react readily with the primary amines (R-NH2) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. The resulting dye conjugates are quite stable.
With EDAC or other equivalent activating coupling agents, fluorescent dyes can react readily with the primary amines (R-NH2) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. The resulting dye conjugates are quite stable.
Ordering information
Price
Catalog Number
Unit Size
Quantity
Add to cart
Additional ordering information
Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
InternationalSee distributors
Bulk requestInquire
Custom sizeInquire
ShippingStandard overnight for United States, inquire for international
Request quotation
Physical properties
Molecular weight~1600
SolventDMSO
Spectral properties
Absorbance (nm)401
Correction Factor (260 nm)1.326
Correction Factor (280 nm)0.543
Extinction coefficient (cm -1 M -1)180001
Excitation (nm)402
Emission (nm)535
Quantum yield0.211
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
Related products
mFluor™ Violet 450 SE
mFluor™ Violet 510 SE
mFluor™ Blue 570 SE
mFluor™ Green 620 SE
mFluor™ Yellow 630 SE
mFluor™ Red 700 SE
mFluor™ Red 780 SE
mFluor™ Red 780 amine
mFluor™ Violet 450-VAD-FMK
mFluor™ 510-VAD-FMK
mFluor™ Violet 450-streptavidin conjugate
mFluor™ Violet 510-streptavidin conjugate
mFluor™ Violet 540-streptavidin conjugate
mFluor™ Blue 570-streptavidin conjugate
mFluor™ Green 620-streptavidin conjugate
mFluor™ Yellow 630-streptavidin conjugate
mFluor™ Red 700-streptavidin conjugate
mFluor™ Red 780-streptavidin conjugate
mFluor™ Violet 450-dUTP *1 mM in Tris Buffer (pH 7.5)*
mFluor™ Blue 570 acid
mFluor™ Green 620 acid
mFluor™ Yellow 630 acid
mFluor™ Red 700 acid
mFluor™ Violet 500 SE
mFluor™ UV375 SE
mFluor™ Red 780 acid
mFluor™ UV460 SE
mFluor™ Blue 630 SE
mFluor™ Blue 660 SE
mFluor™ Violet 610 SE
mFluor™ Green 630 SE
mFluor™ Blue 580 SE
mFluor™ Blue 590 SE
mFluor™ Blue 620 SE
mFluor™ Red 780 Maleimide
mFluor™ Violet 550 SE
mFluor™ Violet 505 SE
mFluor™ Violet 590 SE
mFluor™ Violet 545 SE
mFluor™ UV420 SE
mFluor™ UV455 SE
mFluor™ UV520 SE
mFluor™ UV540 SE
mFluor™ UV610 SE
mFluor™ Violet 450 Azide
mFluor™ Violet 450 maleimide
mFluor™ UV 375 Biotin Conjugate
mFluor™ UV 460 Biotin Conjugate
mFluor™ Violet 500 Biotin Conjugate
mFluor™ Violet 540 Biotin Conjugate
mFluor™ Red 780 Biotin Conjugate
mFluor™ Violet 530 SE
mFluor™ Violet 530 maleimide
mFluor™ Violet 480 SE
mFluor™ Blue 660 tyramide
mFluor™ Violet 450-PEG4-Biotin Conjugate
mFluor™ Violet 450-Wheat Germ Agglutinin (WGA) Conjugate
mFluor™ Violet 500-Wheat Germ Agglutinin (WGA) Conjugate
mFluor™ Violet 540-Wheat Germ Agglutinin (WGA) Conjugate
mFluor™ Blue 585 SE
mFluor™ Blue 583 SE
mFluor™ Blue 580 Styramide
mFluor™ Blue 630 Styramide
mFluor™ Blue 660 Styramide
mFluor™ Green 620 Styramide
mFluor™ Red 780 Styramide
mFluor™ Violet 545 Styramide
mFluor™ Violet 610 Styramide
mFluor™ UV455-streptavidin conjugate
mFluor™ Violet 545-streptavidin conjugate
mFluor™ Violet 550-streptavidin conjugate
mFluor™ Violet 590-streptavidin conjugate
mFluor™ Violet 610-streptavidin conjugate
mFluor™ UV 375 goat anti-mouse IgG (H+L)
mFluor™ UV 375 goat anti-rabbit IgG (H+L)
mFluor™ UV 375 goat anti-rabbit IgG (H+L) *Cross-Absorbed*
mFluor™ UV 375 goat anti-mouse IgG (H+L) *Cross-Absorbed*
mFluor™ Violet 510 goat anti-mouse IgG (H+L)
mFluor™ Violet 510 goat anti-mouse IgG (H+L) *Cross-Absorbed*
mFluor™ Violet 510 goat anti-rabbit IgG (H+L)
mFluor™ Violet 510 goat anti-rabbit IgG (H+L) *Cross-Absorbed*
mFluor™ Red 780 goat anti-rabbit IgG (H+L) *Cross-Absorbed*
mFluor™ Red 780 goat anti-rabbit IgG (H+L)
mFluor™ Red 780 goat anti-mouse IgG (H+L) *Cross-Absorbed*
mFluor™ Red 780 goat anti-mouse IgG (H+L)
mFluor™ Blue 585 Anti-human CD4 Antibody *SK3*
mFluor™ UV420-streptavidin conjugate
Show More (77)

OverviewpdfSDSpdfProtocol


Molecular weight
~1600
Absorbance (nm)
401
Correction Factor (260 nm)
1.326
Correction Factor (280 nm)
0.543
Extinction coefficient (cm -1 M -1)
180001
Excitation (nm)
402
Emission (nm)
535
Quantum yield
0.211
AAT Bioquest's mFluor™ dyes are developed for multicolor flow cytometry-focused applications. These dyes have large Stokes Shifts, and can be well excited by the laser liness of flow cytometers (e.g., 405 nm, 488 nm and 633 nm). mFluor™ Violet 540 dyes have fluorescence excitation and emission maxima of ~405 nm and ~540 nm respectively. These spectral characteristics make them an excellent replacement for Pacific Orange™ labeling dye. mFluor™ Violet 540 acid is stable, and it is a common precursor for making a variety of mFluor™ Violet 540 derivatives.

Spectrum


Open in Advanced Spectrum Viewer
spectrum

Spectral properties

Absorbance (nm)401
Correction Factor (260 nm)1.326
Correction Factor (280 nm)0.543
Extinction coefficient (cm -1 M -1)180001
Excitation (nm)402
Emission (nm)535
Quantum yield0.211

Product Family


NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)
mFluor™ Violet 540 SE4025351800010.2111.3260.543
mFluor™ Violet 450 acid4064453500010.8110.3380.078
mFluor™ Violet 510 acid4125052500010.8610.4640.366
mFluor™ Violet 540 Styramide4025351800010.2111.3260.543

Images


Citations


View all 3 citations: Citation Explorer
Deep Sequencing Analysis of the Eha-Regulated Transcriptome of Edwardsiella tarda Following Acidification
Authors: Gao, D and Liu, N and Li, Y and Zhang, Y and Liu, G and others, undefined
Journal: Metabolomics (Los Angel) (2017): 2153--0769
Suramin inhibits cullin-RING E3 ubiquitin ligases
Authors: Wu, Kenneth and Chong, Robert A and Yu, Qing and Bai, Jin and Spratt, Donald E and Ching, Kevin and Lee, Chan and Miao, Haibin and Tappin, Inger and Hurwitz, Jerard and others, undefined
Journal: Proceedings of the National Academy of Sciences (2016): E2011--E2018
Glycosaminoglycan mimicry by COAM reduces melanoma growth through chemokine induction and function
Authors: Piccard, Helene and Berghmans, Nele and Korpos, Eva and Dillen, Chris and Aelst, Ilse Van and Li, S and ra , undefined and Martens, Erik and Liekens, S and ra , undefined and Noppen, Sam and Damme, Jo Van and others, undefined
Journal: International Journal of Cancer (2012): E425--E436

References


View all 49 references: Citation Explorer
Sequential ordering among multicolor fluorophores for protein labeling facility via aggregation-elimination based beta-lactam probes
Authors: Sadhu KK, Mizukami S, Watanabe S, Kikuchi K.
Journal: Mol Biosyst (2011): 1766
Visualizing dengue virus through Alexa Fluor labeling
Authors: Zhang S, Tan HC, Ooi EE.
Journal: J Vis Exp. (2011)
Fluorescent "Turn-on" system utilizing a quencher-conjugated peptide for specific protein labeling of living cells
Authors: Arai S, Yoon SI, Murata A, Takabayashi M, Wu X, Lu Y, Takeoka S, Ozaki M.
Journal: Biochem Biophys Res Commun (2011): 211
Neuroanatomical basis of clinical joint application of "Jinggu" (BL 64, a source-acupoint) and "Dazhong" (KI 4, a Luo-acupoint) in the rat: a double-labeling study of cholera toxin subunit B conjugated with Alexa Fluor 488 and 594
Authors: Cui JJ, Zhu XL, Ji CF, Jing XH, Bai WZ.
Journal: Zhen Ci Yan Jiu (2011): 262
Simultaneous detection of virulence factors from a colony in diarrheagenic Escherichia coli by a multiplex PCR assay with Alexa Fluor-labeled primers
Authors: Kuwayama M, Shigemoto N, Oohara S, Tanizawa Y, Yamada H, Takeda Y, Matsuo T, Fukuda S.
Journal: J Microbiol Methods (2011): 119
Alexa Fluor 546-ArIB[V11L;V16A] is a potent ligand for selectively labeling alpha 7 nicotinic acetylcholine receptors
Authors: Hone AJ, Whiteaker P, Mohn JL, Jacob MH, McIntosh JM.
Journal: J Neurochem (2010): 994
Asymmetric trimethine 3H-indocyanine dyes: efficient synthesis and protein labeling
Authors: Song F, Wang L, Qiao X, Wang B, Sun S, Fan J, Zhang L, Peng X.
Journal: Org Biomol Chem (2010): 4249
Neuroanatomical characteristics of acupoint "Chengshan" (BL 57) in the rat: a cholera toxin subunit B conjugated with Alexa Fluor 488 method study
Authors: Zhu XL, Bai WZ, Wu FD, Jiang J, Jing XH.
Journal: Zhen Ci Yan Jiu (2010): 433
Photoactivatable and photoconvertible fluorescent probes for protein labeling
Authors: Maurel D, Banala S, Laroche T, Johnsson K.
Journal: ACS Chem Biol (2010): 507
Novel Alexa Fluor-488 labeled antagonist of the A(2A) adenosine receptor: Application to a fluorescence polarization-based receptor binding assay
Authors: Kecskes M, Kumar TS, Yoo L, Gao ZG, Jacobson KA.
Journal: Biochem Pharmacol (2010): 506