MycoLight™ Green JJ98 5 mM in DMSO

<em>E.Coli</em> were stained with 5 uM of MycoLight™ Green JJ98 for 30 minutes and imaged with FITC channel.

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Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
Quotation	Request
International	See distributors
Shipping	Standard overnight for United States, inquire for international

Physical properties

Molecular weight	534
Solvent	DMSO

Spectral properties

Excitation (nm)	482
Emission (nm)	512

Storage, safety and handling

Certificate of Origin	Download PDF
H-phrase	H301, H311, H331
Hazard symbol	T
Intended use	Research Use Only (RUO)
R-phrase	R23, R24, R25
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

Alternative formats

MycoLight™ Green JJ98

Overview SDS Protocol

Molecular weight

534

Excitation (nm)

482

Emission (nm)

512

MycoLight™ Green JJ98 is a green-fluorescent nuclear and chromosome stain that is permeant to both prokaryotic and eukaryotic cell membranes. MycoLight™ Green JJ98 has a high affinity for DNA, and exhibits enhanced fluorescence upon binding with an excitation maximum close to the 488 nm argon laser line and fluorescence emission maximum at ~500 nm. MycoLight™ Green JJ98 is particularly useful as a nuclear counterstain for bacterial assays since it stains both live and dead gram-positive and gram-negative bacteria. It is an excellent replacement for SYTO® 9 (SYTO® is the trademark of Invitrogen).

Platform

Flow cytometer

Excitation	488 nm laser
Emission	530/30 nm filter
Instrument specification(s)	FITC channel

Fluorescence microscope

Excitation	FITC filter set
Emission	FITC filter set
Recommended plate	Black wall/clear bottom

Example protocol

SAMPLE EXPERIMENTAL PROTOCOL

The following protocol can be adapted for most cell types. These conditions require adjustment for each cell type and experimental system. Growth medium, cell density, the presence of other cell types and factors may influence staining. Residual detergent on glassware may also affect staining of many organisms, and cause brightly stained material to appear in solutions with or without cells present.
Use plastic tubes when diluting MycoLight™ Green JJ98, because the diluted stain adheres to glass. In general, the best results are obtained in buffers that do not contain phosphate.
Table 1.Suggested conditions for staining cells with MycoLight™ Green JJ98

Application	Concentration	Staining Conditions
Bacterial cells	50 nM – 20 μM	Vortex to mix, then incubate for 1–30 minutes.
Eukaryotic cells	10 nM – 5 μM	Incubate for 10–120 minutes.
Microarrays	50 nM in TE buffer	Incubate for 5 minutes, rinse and then dry.

Adherent cells in culture may be stained in situ on coverslips. Pellet cells in suspension by centrifugation and resuspend in buffered salt solution or water.
Dilute the MycoLight™ Green JJ98 with non-phosphate buffer such as Hepes buffer or buffer of your choice. Add MycoLight™ Green JJ98 using the concentrations listed in Table 1 as a guideline.
Note In initial experiments, it may be best to try several dye concentrations over the entire suggested range to determine the concentration that yields optimal staining.
Stained eukaryotic cells generally show diffuse cytoplasmic staining as well as nuclear staining. Particularly MycoLight™ Green JJ98 show intense staining of intranuclear bodies frequently.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of MycoLight™ Green JJ98 *5 mM in DMSO* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	187.266 µL	936.33 µL	1.873 mL	9.363 mL	18.727 mL
5 mM	37.453 µL	187.266 µL	374.532 µL	1.873 mL	3.745 mL
10 mM	18.727 µL	93.633 µL	187.266 µL	936.33 µL	1.873 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
	/		=		x		=

Spectrum

Open in Advanced Spectrum Viewer

Spectral properties

Excitation (nm)	482
Emission (nm)	512

Product Family

Name	Excitation (nm)	Emission (nm)
MycoLight™ Green JJ99 5 mM in DMSO	482	512

Images

Figure 1. E.Coli were stained with 5 uM of MycoLight™ Green JJ98 for 30 minutes and imaged with FITC channel.

Figure 2. E.Coli were stained with 5 uM of MycoLight™ Green JJ98 for 30 minutes and imaged with FITC channel.

References

View all 25 references: Citation Explorer

A loop-mediated isothermal amplification (LAMP) assay for Strongyloides stercoralis in stool that uses a visual detection method with SYTO-82 fluorescent dye
Authors: Watts MR, James G, Sultana Y, Ginn AN, Outhred AC, Kong F, Verweij JJ, Iredell JR, Chen SC, Lee R.
Journal: Am J Trop Med Hyg (2014): 306

A new triplex real time PCR which distinguishes between MRSA, MSSA, and mecA coagulase negative strains by means of melting point analysis using SYTO 9
Authors: Weidner J, Cassens U, Gohde W, Wullenweber J, Greve B.
Journal: Clin Lab (2013): 795

Compatibility of SYTO 13 and Hoechst 33342 for longitudinal imaging of neuron viability and cell death
Authors: Hubbard KS, Gut IM, Scheeler SM, Lyman ME, McNutt PM.
Journal: BMC Res Notes (2012): 437

Evaluation of Pseudomonas aeruginosa (PAO1) adhesion to human alveolar epithelial cells A549 using SYTO 9 dye
Authors: Larrosa M, Truchado P, Espin JC, Tomas-Barberan FA, Allende A, Garcia-Conesa MT.
Journal: Mol Cell Probes (2012): 121

Rapid quantification of cell viability and apoptosis in B-cell lymphoma cultures using cyanine SYTO probes
Authors: Wlodkowic D, Skommer J, Darzynkiewicz Z.
Journal: Methods Mol Biol (2011): 81

SYTO dyes and EvaGreen outperform SYBR Green in real-time PCR
Authors: Eischeid AC., undefined
Journal: BMC Res Notes (2011): 263

Use of SYTO 13, a fluorescent dye binding nucleic acids, for the detection of microparticles in in vitro systems
Authors: Ullal AJ, Pisetsky DS, Reich CF, 3rd.
Journal: Cytometry A (2010): 294

Detection of methicillin-resistant Staphylococcus aureus using double duplex real-time PCR and dye Syto 9
Authors: Seputiene V, Vilkoicaite A, Armalyte J, Pavilonis A, Suziedeliene E.
Journal: Folia Microbiol (Praha) (2010): 502

Validation of SYTO 9/propidium iodide uptake for rapid detection of viable but noncultivable Legionella pneumophila
Authors: Giao MS, Wilks SA, Azevedo NF, Vieira MJ, Keevil CW.
Journal: Microb Ecol (2009): 56

Quantitative measurement of Plasmodium-infected erythrocytes in murine models of malaria by flow cytometry using bidimensional assessment of SYTO-16 fluorescence
Authors: Jimenez-Diaz MB, Mulet T, Gomez V, Viera S, Alvarez A, Garuti H, Vazquez Y, Fern and ez A, Ibanez J, Jimenez M, Gargallo-Viola D, Angulo-Barturen I.
Journal: Cytometry A (2009): 225