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MycoLight™ Green JJ98 *5 mM in DMSO*

MycoLight™ Green JJ98 is a green-fluorescent nuclear and chromosome stain that is permeant to both prokaryotic and eukaryotic cell membranes. MycoLight™ Green JJ98 has a high affinity for DNA, and exhibits enhanced fluorescence upon binding with an excitation maximum close to the 488 nm argon laser line and fluorescence emission maximum at ~500 nm. MycoLight™ Green JJ98 is particularly useful as a nuclear counterstain for bacterial assays since it stains both live and dead gram-positive and gram-negative bacteria. It is an excellent replacement for SYTO® 9 (SYTO® is the trademark of Invitrogen).

Example protocol

SAMPLE EXPERIMENTAL PROTOCOL

The following protocol can be adapted for most cell types. These conditions require adjustment for each cell type and experimental system. Growth medium, cell density, the presence of other cell types and factors may influence staining. Residual detergent on glassware may also affect staining of many organisms, and cause brightly stained material to appear in solutions with or without cells present.
Use plastic tubes when diluting MycoLight™ Green JJ98, because the diluted stain adheres to glass. In general, the best results are obtained in buffers that do not contain phosphate.
Table 1.Suggested conditions for staining cells with MycoLight™ Green JJ98
Application Concentration Staining Conditions
Bacterial cells50 nM – 20 μMVortex to mix, then incubate for 1–30 minutes.
Eukaryotic cells10 nM – 5 μMIncubate for 10–120 minutes.
Microarrays50 nM in TE bufferIncubate for 5 minutes, rinse and then dry.
  1. Adherent cells in culture may be stained in situ on coverslips. Pellet cells in suspension by centrifugation and resuspend in buffered salt solution or water.
  2. Dilute the MycoLight™ Green JJ98 with non-phosphate buffer such as Hepes buffer or buffer of your choice. Add MycoLight™ Green JJ98 using the concentrations listed in Table 1 as a guideline.
    Note     In initial experiments, it may be best to try several dye concentrations over the entire suggested range to determine the concentration that yields optimal staining.
  3. Stained eukaryotic cells generally show diffuse cytoplasmic staining as well as nuclear staining. Particularly MycoLight™ Green JJ98 show intense staining of intranuclear bodies frequently. 

Spectrum

Product family

NameExcitation (nm)Emission (nm)
MycoLight™ Green JJ99 *5 mM in DMSO*482512

Citations

View all 1 citations: Citation Explorer
Synthesis and synergistic antibacterial efficiency of chitosan-copper oxide nanocomposites
Authors: Laanoja, J{\"u}ri and Sihtm{\"a}e, Mariliis and Vihodceva, Svetlana and Iesalnieks, Mairis and Otsus, Maarja and Kurvet, Imbi and Kahru, Anne and Kasemets, Kaja
Journal: Heliyon (2024)

References

View all 25 references: Citation Explorer
A loop-mediated isothermal amplification (LAMP) assay for Strongyloides stercoralis in stool that uses a visual detection method with SYTO-82 fluorescent dye
Authors: Watts MR, James G, Sultana Y, Ginn AN, Outhred AC, Kong F, Verweij JJ, Iredell JR, Chen SC, Lee R.
Journal: Am J Trop Med Hyg (2014): 306
A new triplex real time PCR which distinguishes between MRSA, MSSA, and mecA coagulase negative strains by means of melting point analysis using SYTO 9
Authors: Weidner J, Cassens U, Gohde W, Wullenweber J, Greve B.
Journal: Clin Lab (2013): 795
Compatibility of SYTO 13 and Hoechst 33342 for longitudinal imaging of neuron viability and cell death
Authors: Hubbard KS, Gut IM, Scheeler SM, Lyman ME, McNutt PM.
Journal: BMC Res Notes (2012): 437
Evaluation of Pseudomonas aeruginosa (PAO1) adhesion to human alveolar epithelial cells A549 using SYTO 9 dye
Authors: Larrosa M, Truchado P, Espin JC, Tomas-Barberan FA, Allende A, Garcia-Conesa MT.
Journal: Mol Cell Probes (2012): 121
Rapid quantification of cell viability and apoptosis in B-cell lymphoma cultures using cyanine SYTO probes
Authors: Wlodkowic D, Skommer J, Darzynkiewicz Z.
Journal: Methods Mol Biol (2011): 81
Page updated on October 12, 2024

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Catalog Number24000
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Physical properties

Molecular weight

534

Solvent

DMSO

Spectral properties

Excitation (nm)

482

Emission (nm)

512

Storage, safety and handling

Certificate of OriginDownload PDF
H-phraseH301, H311, H331
Hazard symbolT
Intended useResearch Use Only (RUO)
R-phraseR23, R24, R25

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12352200

Platform

Flow cytometer

Excitation488 nm laser
Emission530, 30 nm filter
Instrument specification(s)FITC channel

Fluorescence microscope

ExcitationFITC filter set
EmissionFITC filter set
Recommended plateBlack wall, clear bottom