MycoLight™ Green JJ98 *5 mM in DMSO*
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
Quotation | Request |
International | See distributors |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 534 |
Solvent | DMSO |
Spectral properties
Excitation (nm) | 482 |
Emission (nm) | 512 |
Storage, safety and handling
Certificate of Origin | Download PDF |
H-phrase | H301, H311, H331 |
Hazard symbol | T |
Intended use | Research Use Only (RUO) |
R-phrase | R23, R24, R25 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12352200 |
Alternative formats
MycoLight™ Green JJ98 |
Related products
Overview | ![]() ![]() |
See also: Gram Staining, MycoLight™ Dyes and Kits
Molecular weight 534 | Excitation (nm) 482 | Emission (nm) 512 |
MycoLight™ Green JJ98 is a green-fluorescent nuclear and chromosome stain that is permeant to both prokaryotic and eukaryotic cell membranes. MycoLight™ Green JJ98 has a high affinity for DNA, and exhibits enhanced fluorescence upon binding with an excitation maximum close to the 488 nm argon laser line and fluorescence emission maximum at ~500 nm. MycoLight™ Green JJ98 is particularly useful as a nuclear counterstain for bacterial assays since it stains both live and dead gram-positive and gram-negative bacteria. It is an excellent replacement for SYTO® 9 (SYTO® is the trademark of Invitrogen).
Platform
Flow cytometer
Excitation | 488 nm laser |
Emission | 530/30 nm filter |
Instrument specification(s) | FITC channel |
Fluorescence microscope
Excitation | FITC filter set |
Emission | FITC filter set |
Recommended plate | Black wall/clear bottom |
Example protocol
SAMPLE EXPERIMENTAL PROTOCOL
The following protocol can be adapted for most cell types. These conditions require adjustment for each cell type and experimental system. Growth medium, cell density, the presence of other cell types and factors may influence staining. Residual detergent on glassware may also affect staining of many organisms, and cause brightly stained material to appear in solutions with or without cells present.
Use plastic tubes when diluting MycoLight™ Green JJ98, because the diluted stain adheres to glass. In general, the best results are obtained in buffers that do not contain phosphate.
Table 1.Suggested conditions for staining cells with MycoLight™ Green JJ98
Use plastic tubes when diluting MycoLight™ Green JJ98, because the diluted stain adheres to glass. In general, the best results are obtained in buffers that do not contain phosphate.
Table 1.Suggested conditions for staining cells with MycoLight™ Green JJ98
Application | Concentration | Staining Conditions |
Bacterial cells | 50 nM – 20 μM | Vortex to mix, then incubate for 1–30 minutes. |
Eukaryotic cells | 10 nM – 5 μM | Incubate for 10–120 minutes. |
Microarrays | 50 nM in TE buffer | Incubate for 5 minutes, rinse and then dry. |
- Adherent cells in culture may be stained in situ on coverslips. Pellet cells in suspension by centrifugation and resuspend in buffered salt solution or water.
- Dilute the MycoLight™ Green JJ98 with non-phosphate buffer such as Hepes buffer or buffer of your choice. Add MycoLight™ Green JJ98 using the concentrations listed in Table 1 as a guideline.
Note In initial experiments, it may be best to try several dye concentrations over the entire suggested range to determine the concentration that yields optimal staining. - Stained eukaryotic cells generally show diffuse cytoplasmic staining as well as nuclear staining. Particularly MycoLight™ Green JJ98 show intense staining of intranuclear bodies frequently.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of MycoLight™ Green JJ98 *5 mM in DMSO* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 187.266 µL | 936.33 µL | 1.873 mL | 9.363 mL | 18.727 mL |
5 mM | 37.453 µL | 187.266 µL | 374.532 µL | 1.873 mL | 3.745 mL |
10 mM | 18.727 µL | 93.633 µL | 187.266 µL | 936.33 µL | 1.873 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
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Images
References
View all 25 references: Citation Explorer
A loop-mediated isothermal amplification (LAMP) assay for Strongyloides stercoralis in stool that uses a visual detection method with SYTO-82 fluorescent dye
Authors: Watts MR, James G, Sultana Y, Ginn AN, Outhred AC, Kong F, Verweij JJ, Iredell JR, Chen SC, Lee R.
Journal: Am J Trop Med Hyg (2014): 306
Authors: Watts MR, James G, Sultana Y, Ginn AN, Outhred AC, Kong F, Verweij JJ, Iredell JR, Chen SC, Lee R.
Journal: Am J Trop Med Hyg (2014): 306
A new triplex real time PCR which distinguishes between MRSA, MSSA, and mecA coagulase negative strains by means of melting point analysis using SYTO 9
Authors: Weidner J, Cassens U, Gohde W, Wullenweber J, Greve B.
Journal: Clin Lab (2013): 795
Authors: Weidner J, Cassens U, Gohde W, Wullenweber J, Greve B.
Journal: Clin Lab (2013): 795
Compatibility of SYTO 13 and Hoechst 33342 for longitudinal imaging of neuron viability and cell death
Authors: Hubbard KS, Gut IM, Scheeler SM, Lyman ME, McNutt PM.
Journal: BMC Res Notes (2012): 437
Authors: Hubbard KS, Gut IM, Scheeler SM, Lyman ME, McNutt PM.
Journal: BMC Res Notes (2012): 437
Evaluation of Pseudomonas aeruginosa (PAO1) adhesion to human alveolar epithelial cells A549 using SYTO 9 dye
Authors: Larrosa M, Truchado P, Espin JC, Tomas-Barberan FA, Allende A, Garcia-Conesa MT.
Journal: Mol Cell Probes (2012): 121
Authors: Larrosa M, Truchado P, Espin JC, Tomas-Barberan FA, Allende A, Garcia-Conesa MT.
Journal: Mol Cell Probes (2012): 121
Rapid quantification of cell viability and apoptosis in B-cell lymphoma cultures using cyanine SYTO probes
Authors: Wlodkowic D, Skommer J, Darzynkiewicz Z.
Journal: Methods Mol Biol (2011): 81
Authors: Wlodkowic D, Skommer J, Darzynkiewicz Z.
Journal: Methods Mol Biol (2011): 81
SYTO dyes and EvaGreen outperform SYBR Green in real-time PCR
Authors: Eischeid AC., undefined
Journal: BMC Res Notes (2011): 263
Authors: Eischeid AC., undefined
Journal: BMC Res Notes (2011): 263
Use of SYTO 13, a fluorescent dye binding nucleic acids, for the detection of microparticles in in vitro systems
Authors: Ullal AJ, Pisetsky DS, Reich CF, 3rd.
Journal: Cytometry A (2010): 294
Authors: Ullal AJ, Pisetsky DS, Reich CF, 3rd.
Journal: Cytometry A (2010): 294
Detection of methicillin-resistant Staphylococcus aureus using double duplex real-time PCR and dye Syto 9
Authors: Seputiene V, Vilkoicaite A, Armalyte J, Pavilonis A, Suziedeliene E.
Journal: Folia Microbiol (Praha) (2010): 502
Authors: Seputiene V, Vilkoicaite A, Armalyte J, Pavilonis A, Suziedeliene E.
Journal: Folia Microbiol (Praha) (2010): 502
Validation of SYTO 9/propidium iodide uptake for rapid detection of viable but noncultivable Legionella pneumophila
Authors: Giao MS, Wilks SA, Azevedo NF, Vieira MJ, Keevil CW.
Journal: Microb Ecol (2009): 56
Authors: Giao MS, Wilks SA, Azevedo NF, Vieira MJ, Keevil CW.
Journal: Microb Ecol (2009): 56
Quantitative measurement of Plasmodium-infected erythrocytes in murine models of malaria by flow cytometry using bidimensional assessment of SYTO-16 fluorescence
Authors: Jimenez-Diaz MB, Mulet T, Gomez V, Viera S, Alvarez A, Garuti H, Vazquez Y, Fern and ez A, Ibanez J, Jimenez M, Gargallo-Viola D, Angulo-Barturen I.
Journal: Cytometry A (2009): 225
Authors: Jimenez-Diaz MB, Mulet T, Gomez V, Viera S, Alvarez A, Garuti H, Vazquez Y, Fern and ez A, Ibanez J, Jimenez M, Gargallo-Viola D, Angulo-Barturen I.
Journal: Cytometry A (2009): 225