Name: PhosphoWorks™ Fluorimetric Pyrophosphate Assay Kit *Blue Fluorescence*
Brand: AAT Bioquest
SKU: 21611
Price: 237 USD
Availability: InStock

PhosphoWorks™ Fluorimetric Pyrophosphate Assay Kit

Blue Fluorescence

PhosphoWorks™ Fluorimetric Pyrophosphate Assay Kit *Blue Fluorescence* is a fluorimetric assay kit for quantifying pyrophosphate (PPi) using a microplate assay.

Detection Principle: Fluorescence increases proportionally with PPi concentration.
Sensitivity: Detects PPi down to 1 µM (100 pmol/well) after 10 minutes.
Detection Chemistry: Uses a proprietary fluorogenic PPi sensor.
Workflow: Direct assay that avoids multi-enzyme coupling methods.
Instrument Compatibility: Fluorescence detection at Ex/Em 316/456 nm using solid black plates.

Pyrophosphate, ATP and phosphate dose responses were measured with PhosphoWorks™ Fluoremetric Pyrophosphate Assay Kit in a solid black 96-well plate using a fluorescence microplate reader.

Protocol

SDS

Catalog	Size	Price	Quantity
21611	200 Tests	Price

Overview

Pyrophosphate (PPi) is produced by a number of biochemical reactions, such as ATP hydrolysis, DNA and RNA polymerizations, cyclic AMP formation by the enzyme adenylate cyclase and the enzymatic activation of fatty acids to form their coenzyme A esters. Our PhosphoWroks™ Pyrophosphate Assay Kit provides the most robust spectrophotometric method for measuring pyrophosphate. This kit uses our proprietary fluorogenic pyrophosphate sensor that has its fluorescence intensity proportionally dependent upon the concentration of pyrophosphate. Our assay is much easier and more robust than the enzyme-coupling pyrophosphate methods that require at least two enzymes for their pyrophosphate detections. The kit provides all the essential components for assaying pyrophosphate. This kit has been successfully used in high throughput screening (HTS). Please inquire special HTS bulk package discount for the screening of >10,000 assays.

Citations

View all 13 citations: Citation Explorer

Modulation of host ATP levels by secreted bacterial effectors

Authors:

He, Chunlin and Li, Chuang and Liu, Yao and Chen, Tao-Tao and Li, Chunxiuli and Chu, Xiao and Liu, Shuxin and Wang, Lidong and Zhang, Yong and Ouyang, Songying and others,

Journal:

Nature Communications (2025): 1--15

Inorganic Pyrophosphate at Serum Concentration May Not Be Able to Inhibit Mineralization: A Study in Aqueous Solutions and Serum

Authors:

Cheng, Yuxuan and Ru, Jing and Feng, Chaobo and Liu, Xiaohao and Zeng, Hua and Tan, Shuo and Chen, Xi and Chen, Feng and Lu, Bing-Qiang

Journal:

ACS Omega (2024)

AaTAS1 and AaMFS1 Genes for Biosynthesis or Efflux Transport of Tenuazonic Acid and Pathogenicity of Alternaria alternata

Authors:

Sun, Fan and Cao, Xueqiang and Yu, Dianzhen and Hu, Dongqiang and Yan, Zheng and Fan, Yingying and Wang, Cheng and Wu, Aibo

Journal:

Molecular Plant-Microbe Interactions (2022): 416--427

Augmented fibroblast growth factor-23 secretion in bone locally contributes to impaired bone mineralization in chronic kidney disease in mice

Authors:

Andrukhova, Olena and Sch{\"u}ler, Christiane and Bergow, Claudia and Petric, Alexandra and Erben, Reinhold G

Journal:

Frontiers in endocrinology (2018): 311

RANKL Expression Is Increased in Circulating Mononuclear Cells of Patients with Calcific Aortic Stenosis

Authors:

Rattazzi, Marcello and Faggin, Elisabetta and Bertacco, Elisa and Buso, Roberta and Puato, Massimo and Plebani, Mario and Zaninotto, Martina and Condotta, Davide and Zoppellaro, Giacomo and Pagliani, Leopoldo and others, undefined

Journal:

Journal of Cardiovascular Translational Research (2018): 1--10

References

View all 136 references: Citation Explorer

Cell-surface-localized ATP detection with immobilized firefly luciferase

Authors:

Nakamura M, Mie M, Funabashi H, Yamamoto K, Ando J, Kobatake E.

Journal:

Anal Biochem (2006): 61

Ca2+ oscillations stimulate an ATP increase during fertilization of mouse eggs

Authors:

Campbell K, Swann K.

Journal:

Dev Biol (2006): 225

An efficient method for quantitative determination of cellular ATP synthetic activity

Authors:

Hara KY, Mori H.

Journal:

J Biomol Screen (2006): 310

Basic evaluation for new antimicrobial susceptibility testing of Mycobacterium leprae by bioluminescence assay (ATP method)

Authors:

Yamazaki T, Gidoh M, Matsuoka M.

Journal:

Nihon Hansenbyo Gakkai Zasshi (2006): 227

ATP bioluminescence assay for estimation of microbial populations of fresh-cut melon

Authors:

Ukuku DO, Sapers GM, Fett WF.

Journal:

J Food Prot (2005): 2427

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Fluorescence microplate reader
Excitation	316 nm
Emission	456 nm
Cutoff	420 nm
Recommended plate	Solid black

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 15, 2026

Component A: Assay Buffer	1 bottle (25 mL)
Component B: PPi Sensor	1 vial (lyophilized powder)
Component C: Pyrophosphate Standard	1 vial (1 mL, 50 mM)
Component D: DMSO	1 vial (200 µL)