Portelite™ Fluorimetric Protein Quantitation Kit *Optimized for CytoCite™ and Qubit™ Fluorometers*

Portelite™ Fluorimetric Protein Quantitation Kit

Optimized for CytoCite™ and Qubit™ Fluorometers

Protein quantification is an essential task in protein purification, electrophoresis, cell biology, molecular biology and other research applications. Biuret, Lowry, BCA and Bradford assays are routinely used for estimating protein concentration. However, these colorimetric assays are less sensitive, and require large sample volume to ensure accuracy. Our Portelite™ Fluorimetric Protein Quantitation Kit is significantly more sensitive than existing colorimetric protein measurements, e.g., Bradford and Bicinchoninic acid (BCA) assays. Prolite™ Orange used in the kit is non-fluorescent in aqueous solution, but reacts rapidly with proteins and generates bright fluorescence. The Portelite™ Fluorimetric Protein Quantitation Kit provides a simple method for quantifying protein concentration in solutions. The assay has dynamic range from 12.5 ug/mL to 5 mg/mL of BSA. The kit is optimized for Cytocite™ and Qubit™ fluorometers. It can be used for (1) studying protein/protein interactions; (2) measuring column fractions after affinity chromatography; (3) estimating recovery of membrane proteins from cell extract; and (4) high-throughput screening of fusion proteins.

<p>Serial dilutions of BSA, chicken-egg ovalbumin, porcine thyroglobulin were measured at Ex/Em 485/590 nm using Portelite™ Fluorimetric Protein Quantitation Kit *Optimized for CytoCite™ and Qubit™ Fluorometers* with Qubit® Fluorometer. As low as 50 ng/mL of protein can be detected.</p>

Protocol

COA

Catalog	Size	Price
11109	100 Tests	Price
11110	200 Tests	Price
11111	500 Tests	Price

Components

Catalog 11109 (100 Tests)

Component A: Prolite™ Orange (200X)	1 vial (300 µL)
Component B: BSA Standard 1 (0 ng/uL)	1 vial (1 mL)
Component C: BSA Standard 2 (200 ng/uL)	1 vial (1 mL)
Component D: BSA Standard 3 (400 ng/uL)	1 vial (1 mL)
Component E: Sample Dilution Buffer	1 bottle (60 mL)

Catalog 11110 (200 Tests)

Component A: Prolite™ Orange (200X)	1 vial (300 µL)
Component B: BSA Standard 1 (0 ng/uL)	1 vial (1.5 mL)
Component C: BSA Standard 2 (200 ng/uL)	1 vial (1.5 mL)
Component D: BSA Standard 3 (400 ng/uL)	1 vial (1.5 mL)
Component E: Sample Dilution Buffer	1 bottle (100 mL)

Catalog 11111 (500 Tests)

Component A: Prolite™ Orange (200X)	1 vial (1.5 mL)
Component B: BSA Standard 1 (0 ng/uL)	1 bottle (5 mL)
Component C: BSA Standard 2 (200 ng/uL)	1 bottle (5 mL)
Component D: BSA Standard 3 (400 ng/uL)	1 bottle (5 mL)
Component E: Sample Dilution Buffer	300 mL (3 bottles-100 mL each)

References

View all 27 references: Citation Explorer

Use of anchor protein modules in fluorescence polarisation aptamer assay for ochratoxin A determination

Authors:

Samokhvalov, A. V.; Safenkova, I. V.; Eremin, S. A.; Zherdev, A. V.; Dzantiev, B. B.

Journal:

Anal Chim Acta (2017): 80-87

Quantification of Membrane Protein Self-Association with a High-Throughput Compatible Fluorescence Assay

Authors:

Li, J.; Qiu, X. J.

Journal:

Biochemistry (2017): 1951-1954

Dual Amplification Fluorescence Assay for Alpha Fetal Protein Utilizing Immunohybridization Chain Reaction and Metal-Enhanced Fluorescence of Carbon Nanodots

Authors:

Xu, D. D.; Liu, C.; Li, C. Y.; Song, C. Y.; Kang, Y. F.; Qi, C. B.; Lin, Y.; Pang, D. W.; Tang, H. W.

Journal:

ACS Appl Mater Interfaces (2017): 37606-37614

Tryptophan fluorescence quenching as a binding assay to monitor protein conformation changes in the membrane of intact mitochondria

Authors:

Akbar, S. M.; Sreeramulu, K.; Sharma, H. C.

Journal:

J Bioenerg Biomembr (2016): 241-7

Label-free fluorescence assay for protein kinase based on peptide biomineralized gold nanoclusters as signal sensing probe

Authors:

Song, W.; Wang, Y.; Liang, R. P.; Zhang, L.; Qiu, J. D.

Journal:

Biosens Bioelectron (2015): 234-40

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
UNSPSC	12171501

Instrument settings

Qubit Fluorometer
Excitation	480 nm
Emission	510-580 nm
Instrument specification(s)	0.2 mL, thin-wall PCR tube

CytoCite Fluorometer
Excitation	480 nm
Emission	510-580 nm
Instrument specification(s)	0.2 mL, thin-wall PCR tube

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Page updated on October 7, 2025