Protonex™ Red 670 acid

Protonex™ Red 670 works by changing its fluorescence intensity depending on the pH of the environment. Protonex™ Red 670 is minimally fluorescent at a basic pH and maximally fluorescent at an acidic pH. When Protonex™ Red 670 is bound to a receptor or an antibody on the cell surface, it is essentially non-fluorescent because the extracellular pH is neutral. However, when the receptor or antibody is internalized into the cell in response to a stimulus, it enters the endosomal pathway, where the pH is acidic. This causes Protonex™ Red 670 to become highly fluorescent and emit red light when excited by a red laser such as a 632 nm He-Ne or 647 nm red laser. By measuring the fluorescence intensity of Protonex™ Red 670, one can monitor the activation and trafficking of receptors or antibodies in live cells. Protonex™ Red 670 is especially useful in studying the activation and trafficking of G protein-coupled receptors (GPCRs), one of the most popular therapeutic drug targets. Protonex™ Red 670 can be used to label any receptor or epitope tag antibody and monitor its movement from the cell surface into acidic endosomes upon agonist stimulation. Protonex™ Red 670 can also be used to measure high-potency agonist and antagonist responses of different GPCRs in live cells, such as the activation of TRHR-1 and beta-adrenoceptor, two GPCRs that are involved in hormone regulation and cardiovascular function.

Fluorescence response of Protonex™ Red 670 in various pH solutions.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
21181	1 mg	Price

References

View all 50 references: Citation Explorer

Mechanisms of 15-Epi-LXA4-Mediated HO-1 in Cytoprotection Following Inflammatory Injury.

Authors:

Wang, Meng and Tong, Kun and Chen, Zhe and Wen, Zhengde

Journal:

The Journal of surgical research (2023): 245-255

Generalization of the Ratiometric Method to Extend pH Range Measurements of the BCECF Probe.

Authors:

Tafech, Alaa and Beaujean, Céline and Usson, Yves and Stéphanou, Angélique

Journal:

Biomolecules (2023)

The phosphoinositide PI(3,5)P2 inhibits the activity of plant NHX proton/potassium antiporters: Advantages of a novel electrophysiological approach.

Authors:

Gradogna, Antonella and Pardo, José M and Carpaneto, Armando

Journal:

Biomolecular concepts (2022): 119-125

FLIM for Evaluation of Difference in Metabolic Status between Native and Differentiated from iPSCs Dermal Papilla Cells.

Authors:

Kashirina, Alena and Gavrina, Alena and Mozherov, Artem and Kozlov, Dmitriy and Kuznetsova, Daria and Vorotelyak, Ekaterina and Zagaynova, Elena and Kalabusheva, Ekaterina and Kashina, Aleksandra

Journal:

Cells (2022)

mOrange2, a Genetically Encoded, pH Sensitive Fluorescent Protein, is an Alternative to BCECF-AM to Measure Intracellular pH to Determine NHE3 and DRA Activity.

Authors:

Sarker, Rafiquel and Tse, Chung Ming and Lin, Ruxian and McNamara, George and Singh, Varsha and Donowitz, Mark

Journal:

Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology (2022): 39-49

Physical properties

Molecular weight	629.76
Solvent	DMSO

Spectral properties

Excitation (nm)	643
Emission (nm)	660

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

Documents

Protocol
Safety Data Sheet
Certificate of Analysis

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Page updated on October 28, 2025